| 2025-12-29 | BarnsleyvLincolnLeague One · England | 0–2 | League One · England |
| 2025-12-26 | BarnsleyvMansfieldLeague One · England | 2–3 | League One · England |
| 2025-12-20 | ExetervBarnsleyLeague One · England | 3–0 | League One · England |
| 2025-12-13 | BarnsleyvLeyton OrientLeague One · England | 3–2 | League One · England |
| 2025-12-09 | LincolnvBarnsleyLeague One · England | 3–1 | League One · England |
| 2025-11-29 | StockportvBarnsleyLeague One · England | 1–1 | League One · England |
| 2025-11-22 | BarnsleyvLutonLeague One · England | 5–0 | League One · England |
| 2025-11-08 | DoncastervBarnsleyLeague One · England | 1–2 | League One · England |
| 2025-10-25 | BarnsleyvRotherhamLeague One · England | 0–1 | League One · England |
| 2025-10-18 | BradfordvBarnsleyLeague One · England | 2–2 | League One · England |
| 2025-10-04 | WycombevBarnsleyLeague One · England | 2–2 | League One · England |
| 2025-09-27 | BarnsleyvPort ValeLeague One · England | 0–2 | League One · England |
| 2025-09-20 | BlackpoolvBarnsleyLeague One · England | 1–0 | League One · England |
| 2025-09-13 | BarnsleyvReadingLeague One · England | 3–2 | League One · England |
| 2025-08-30 | BarnsleyvHuddersfieldLeague One · England | 3–1 | League One · England |
| 2025-08-23 | AFC WimbledonvBarnsleyLeague One · England | 2–0 | League One · England |
| 2025-08-19 | PeterborovBarnsleyLeague One · England | 0–1 | League One · England |
| 2025-08-16 | BarnsleyvBoltonLeague One · England | 1–1 | League One · England |
| 2025-08-09 | BarnsleyvBurtonLeague One · England | 3–2 | League One · England |
| 2025-08-02 | PlymouthvBarnsleyLeague One · England | 1–3 | League One · England |
| 2025-05-03 | ReadingvBarnsleyLeague One · England | 2–4 | League One · England |
| 2025-04-26 | BarnsleyvShrewsburyLeague One · England | 1–2 | League One · England |
| 2025-04-21 | BarnsleyvPeterboroLeague One · England | 1–1 | League One · England |
| 2025-04-18 | Leyton OrientvBarnsleyLeague One · England | 4–3 | League One · England |
| 2025-04-12 | BarnsleyvBoltonLeague One · England | 4–1 | League One · England |
| 2025-04-05 | BirminghamvBarnsleyLeague One · England | 6–2 | League One · England |
| 2025-04-01 | BarnsleyvExeterLeague One · England | 1–2 | League One · England |
| 2025-03-29 | WiganvBarnsleyLeague One · England | 1–1 | League One · England |
| 2025-03-22 | BarnsleyvCambridgeLeague One · England | 1–1 | League One · England |
| 2025-03-15 | MansfieldvBarnsleyLeague One · England | 2–1 | League One · England |
| 2025-03-08 | BarnsleyvBlackpoolLeague One · England | 0–3 | League One · England |
| 2025-03-04 | CharltonvBarnsleyLeague One · England | 1–0 | League One · England |
| 2025-03-01 | BarnsleyvLincolnLeague One · England | 4–3 | League One · England |
| 2025-02-25 | NorthamptonvBarnsleyLeague One · England | 1–2 | League One · England |
| 2025-02-22 | RotherhamvBarnsleyLeague One · England | 0–1 | League One · England |
| 2025-02-15 | BarnsleyvHuddersfieldLeague One · England | 1–2 | League One · England |
| 2025-02-08 | StockportvBarnsleyLeague One · England | 2–1 | League One · England |
| 2025-02-01 | BarnsleyvBurtonLeague One · England | 0–0 | League One · England |
| 2025-01-28 | WycombevBarnsleyLeague One · England | 2–1 | League One · England |
| 2025-01-25 | BarnsleyvStevenageLeague One · England | 0–1 | League One · England |
| 2025-01-18 | Bristol RvsvBarnsleyLeague One · England | 3–1 | League One · England |
| 2025-01-04 | BarnsleyvCrawley TownLeague One · England | 3–0 | League One · England |
| 2025-01-01 | BarnsleyvWrexhamLeague One · England | 2–1 | League One · England |