| 2010-04-24 | ArsenalvMan CityPremier League · England | 0–0 | Premier League · England |
| 2010-04-24 | WalsallvHartlepoolLeague One · England | 3–1 | League One · England |
| 2010-04-24 | St MirrenvKilmarnockPremiership · Scotland | 1–0 | Premiership · Scotland |
| 2010-04-24 | FalkirkvHamiltonPremiership · Scotland | 0–1 | Premiership · Scotland |
| 2010-04-24 | NorwichvGillinghamLeague One · England | 2–0 | League One · England |
| 2010-04-24 | HullvSunderlandPremier League · England | 0–1 | Premier League · England |
| 2010-04-24 | BetisvElcheLa Liga 2 · Spain | 0–3 | La Liga 2 · Spain |
| 2010-04-24 | DoncastervScunthorpeChampionship · England | 4–3 | Championship · England |
| 2010-04-24 | VicenzavPadovaSerie B · Italy | 0–0 | Serie B · Italy |
| 2010-04-24 | KayserisporvManisasporSüper Lig · Turkey | 1–2 | Süper Lig · Turkey |
| 2010-04-24 | BarcelonavXerezLa Liga · Spain | 3–1 | La Liga · Spain |
| 2010-04-24 | AachenvGreuther Furth2. Bundesliga · Germany | 2–2 | 2. Bundesliga · Germany |
| 2010-04-24 | StirlingvClydeLeague One · Scotland | 1–0 | League One · Scotland |
| 2010-04-24 | StranraervForfarLeague Two · Scotland | 2–0 | League Two · Scotland |
| 2010-04-24 | BoltonvPortsmouthPremier League · England | 2–2 | Premier League · England |
| 2010-04-24 | GatesheadvAFC WimbledonNational League · England | 1–0 | National League · England |
| 2010-04-24 | MainzvEin FrankfurtBundesliga · Germany | 3–3 | Bundesliga · Germany |
| 2010-04-24 | MontrosevLivingstonLeague Two · Scotland | 0–5 | League Two · Scotland |
| 2010-04-24 | MiddlesbroughvCoventryChampionship · England | 1–1 | Championship · England |
| 2010-04-24 | GallipolivSalernitanaSerie B · Italy | 3–2 | Serie B · Italy |
| 2010-04-24 | PeterborovBlackpoolChampionship · England | 0–1 | Championship · England |
| 2010-04-24 | LorientvBordeauxLigue 1 · France | 1–0 | Ligue 1 · France |
| 2010-04-24 | ValenciavLa CorunaLa Liga · Spain | 1–0 | La Liga · Spain |
| 2010-04-24 | MansfieldvKidderminsterNational League · England | 3–3 | National League · England |
| 2010-04-24 | MorecambevDag and RedLeague Two · England | 1–0 | League Two · England |
| 2010-04-24 | BenficavOlhanensePrimeira Liga · Portugal | 5–0 | Primeira Liga · Portugal |
| 2010-04-24 | DarlingtonvGrimsbyLeague Two · England | 0–2 | League Two · England |
| 2010-04-24 | HistonvBarrowNational League · England | 2–2 | National League · England |
| 2010-04-24 | GrossetovLecceSerie B · Italy | 0–3 | Serie B · Italy |
| 2010-04-24 | M'gladbachvBayern MunichBundesliga · Germany | 1–1 | Bundesliga · Germany |
| 2010-04-24 | Rushden & DvLutonNational League · England | 1–1 | National League · England |
| 2010-04-24 | BrentfordvYeovilLeague One · England | 1–1 | League One · England |
| 2010-04-24 | WatfordvReadingChampionship · England | 3–0 | Championship · England |
| 2010-04-24 | SassuolovAnconaSerie B · Italy | 1–0 | Serie B · Italy |
| 2010-04-24 | DumbartonvArbroathLeague One · Scotland | 0–2 | League One · Scotland |
| 2010-04-24 | AlbinoleffevFrosinoneSerie B · Italy | 4–1 | Serie B · Italy |
| 2010-04-24 | SochauxvBoulogneLigue 1 · France | 0–3 | Ligue 1 · France |
| 2010-04-24 | CrewevAccringtonLeague Two · England | 5–1 | League Two · England |
| 2010-04-24 | ChesterfieldvBradfordLeague Two · England | 1–1 | League Two · England |
| 2010-04-24 | NicevGrenobleLigue 1 · France | 2–1 | Ligue 1 · France |
| 2010-04-24 | LeedsvMilton Keynes DonsLeague One · England | 4–1 | League One · England |
| 2010-04-24 | East StirlingvAnnan AthleticLeague Two · Scotland | 3–1 | League Two · Scotland |
| 2010-04-24 | CardiffvSheffield WedsChampionship · England | 3–2 | Championship · England |
| 2010-04-24 | BerwickvAlbion RvsLeague Two · Scotland | 1–2 | League Two · Scotland |
| 2010-04-24 | IntervAtalantaSerie A · Italy | 3–1 | Serie A · Italy |
| 2010-04-24 | St JohnstonevAberdeenPremiership · Scotland | 1–1 | Premiership · Scotland |
| 2010-04-24 | DenizlisporvAnkaragucuSüper Lig · Turkey | 0–0 | Süper Lig · Turkey |
| 2010-04-24 | CastellonvLas PalmasLa Liga 2 · Spain | 2–0 | La Liga 2 · Spain |
| 2010-04-24 | Paris SGvRennesLigue 1 · France | 1–1 | Ligue 1 · France |
| 2010-04-24 | NorthamptonvShrewsburyLeague Two · England | 2–0 | League Two · England |