| 2016-09-10 | ZwollevUtrechtEredivisie · Netherlands | 1–1 | Eredivisie · Netherlands |
| 2016-09-10 | MorecambevDoncasterLeague Two · England | 1–5 | League Two · England |
| 2016-09-10 | LutonvGrimsbyLeague Two · England | 1–2 | League Two · England |
| 2016-09-10 | MechelenvSt TruidenPro League · Belgium | 2–0 | Pro League · Belgium |
| 2016-09-10 | StevenagevCrawley TownLeague Two · England | 2–1 | League Two · England |
| 2016-09-10 | OxfordvSwindonLeague One · England | 2–0 | League One · England |
| 2016-09-10 | FC TokyovShonan BellmareJ1 League · Japan | 3–0 | J1 League · Japan |
| 2016-09-10 | CrewevExeterLeague Two · England | 2–0 | League Two · England |
| 2016-09-10 | DunfermlinevDundee UnitedChampionship · Scotland | 1–3 | Championship · Scotland |
| 2016-09-10 | BaselvGrasshoppersSuper League · Switzerland | 3–1 | Super League · Switzerland |
| 2016-09-10 | FC VoluntarivPoli TimisoaraSuperliga · Romania | 4–1 | Superliga · Romania |
| 2016-09-10 | St MirrenvQueen of SthChampionship · Scotland | 1–3 | Championship · Scotland |
| 2016-09-10 | BresciavPerugiaSerie B · Italy | 1–1 | Serie B · Italy |
| 2016-09-10 | Kawasaki FrontalevAvispa FukuokaJ1 League · Japan | 3–1 | J1 League · Japan |
| 2016-09-10 | Le HavrevValenciennesLigue 2 · France | 2–2 | Ligue 2 · France |
| 2016-09-10 | Man UnitedvMan CityPremier League · England | 1–2 | Premier League · England |
| 2016-09-10 | StokevTottenhamPremier League · England | 0–4 | Premier League · England |
| 2016-09-10 | Tianjin Jinmen TigervHenan Songshan LongmenSuper League · China | 2–1 | Super League · China |
| 2016-09-10 | LevantevZaragozaLa Liga 2 · Spain | 4–2 | La Liga 2 · Spain |
| 2016-09-10 | LiverpoolvLeicesterPremier League · England | 4–1 | Premier League · England |
| 2016-09-10 | Braintree TownvGatesheadNational League · England | 1–4 | National League · England |
| 2016-09-10 | AltachvMattersburgBundesliga · Austria | 2–1 | Bundesliga · Austria |
| 2016-09-10 | Cruz AzulvClub AmericaLiga MX · Mexico | 3–4 | Liga MX · Mexico |
| 2016-09-10 | KortrijkvWesterloPro League · Belgium | 4–1 | Pro League · Belgium |
| 2016-09-10 | WolvesvBurtonChampionship · England | 1–1 | Championship · England |
| 2016-09-10 | NorwichvCardiffChampionship · England | 3–2 | Championship · England |
| 2016-09-10 | FrosinonevLatinaSerie B · Italy | 2–1 | Serie B · Italy |
| 2016-09-10 | Piast GliwicevLecznaEkstraklasa · Poland | 3–3 | Ekstraklasa · Poland |
| 2016-09-10 | SevillavLas PalmasLa Liga · Spain | 2–1 | La Liga · Spain |
| 2016-09-10 | KerkyravPlataniasSuper League · Greece | 0–1 | Super League · Greece |
| 2016-09-10 | WrexhamvSuttonNational League · England | 1–0 | National League · England |
| 2016-09-10 | LevadeiakosvPanathinaikosSuper League · Greece | 0–3 | Super League · Greece |
| 2016-09-10 | GillinghamvBradfordLeague One · England | 1–1 | League One · England |
| 2016-09-10 | BarcelonavAlavesLa Liga · Spain | 1–2 | La Liga · Spain |
| 2016-09-10 | DerbyvNewcastleChampionship · England | 0–2 | Championship · England |
| 2016-09-10 | CSKA MoscowvAkhmat GroznyPremier League · Russia | 3–0 | Premier League · Russia |
| 2016-09-10 | BarrowvBoreham WoodNational League · England | 1–1 | National League · England |
| 2016-09-10 | BrannvVikingEliteserien · Norway | 0–0 | Eliteserien · Norway |
| 2016-09-10 | IwatavVissel KobeJ1 League · Japan | 3–4 | J1 League · Japan |
| 2016-09-10 | PortovGuimaraesPrimeira Liga · Portugal | 3–0 | Primeira Liga · Portugal |
| 2016-09-10 | St. PoltenvRiedBundesliga · Austria | 2–3 | Bundesliga · Austria |
| 2016-09-10 | FreiburgvM'gladbachBundesliga · Germany | 3–1 | Bundesliga · Germany |
| 2016-09-10 | Ross CountyvMotherwellPremiership · Scotland | 1–1 | Premiership · Scotland |
| 2016-09-10 | OrenburgvFK Anzi MakhackalaPremier League · Russia | 0–0 | Premier League · Russia |
| 2016-09-10 | Annan AthleticvForfarLeague Two · Scotland | 1–2 | League Two · Scotland |
| 2016-09-10 | DundeevKilmarnockPremiership · Scotland | 1–1 | Premiership · Scotland |
| 2016-09-10 | GuiseleyvWokingNational League · England | 1–1 | National League · England |
| 2016-09-10 | CeltavAth MadridLa Liga · Spain | 0–4 | La Liga · Spain |
| 2016-09-10 | StirlingvEdinburgh CityLeague Two · Scotland | 1–1 | League Two · Scotland |
| 2016-09-10 | AdanasporvGaziantepsporSüper Lig · Turkey | 0–0 | Süper Lig · Turkey |