| 2010-05-08 | ValenciennesvLyonLigue 1 · France | 2–2 | Ligue 1 · France |
| 2010-05-08 | BurtonvGrimsbyLeague Two · England | 3–0 | League Two · England |
| 2010-05-08 | ValladolidvSantanderLa Liga · Spain | 2–1 | La Liga · Spain |
| 2010-05-08 | FreiburgvDortmundBundesliga · Germany | 3–1 | Bundesliga · Germany |
| 2010-05-08 | MorecambevAldershotLeague Two · England | 1–0 | League Two · England |
| 2010-05-08 | MantovavModenaSerie B · Italy | 1–1 | Serie B · Italy |
| 2010-05-08 | St MirrenvAberdeenPremiership · Scotland | 0–1 | Premiership · Scotland |
| 2010-05-08 | StockportvTranmereLeague One · England | 0–3 | League One · England |
| 2010-05-08 | Real UnionvSociedadLa Liga 2 · Spain | 0–0 | La Liga 2 · Spain |
| 2010-05-08 | SassuolovTorinoSerie B · Italy | 2–3 | Serie B · Italy |
| 2010-05-08 | LeixoesvSp LisbonPrimeira Liga · Portugal | 1–2 | Primeira Liga · Portugal |
| 2010-05-08 | Sp GijonvAth MadridLa Liga · Spain | 1–1 | La Liga · Spain |
| 2010-05-08 | CartagenavNumanciaLa Liga 2 · Spain | 2–1 | La Liga 2 · Spain |
| 2010-05-08 | Villarreal BvLas PalmasLa Liga 2 · Spain | 2–5 | La Liga 2 · Spain |
| 2010-05-08 | Real MadridvAth BilbaoLa Liga · Spain | 5–1 | La Liga · Spain |
| 2010-05-08 | TenerifevAlmeriaLa Liga · Spain | 2–2 | La Liga · Spain |
| 2010-05-08 | ColchestervLeyton OrientLeague One · England | 1–0 | League One · England |
| 2010-05-08 | WolfsburgvEin FrankfurtBundesliga · Germany | 3–1 | Bundesliga · Germany |
| 2010-05-08 | SevillavBarcelonaLa Liga · Spain | 2–3 | La Liga · Spain |
| 2010-05-08 | LincolnvMacclesfieldLeague Two · England | 0–0 | League Two · England |
| 2010-05-08 | LillevMarseilleLigue 1 · France | 3–2 | Ligue 1 · France |
| 2010-05-08 | VicenzavEmpoliSerie B · Italy | 2–1 | Serie B · Italy |
| 2010-05-08 | XerezvZaragozaLa Liga · Spain | 3–2 | La Liga · Spain |
| 2010-05-08 | HerthavBayern MunichBundesliga · Germany | 1–3 | Bundesliga · Germany |
| 2010-05-08 | St JohnstonevHamiltonPremiership · Scotland | 2–3 | Premiership · Scotland |
| 2010-05-08 | NorwichvCarlisleLeague One · England | 0–2 | League One · England |
| 2010-05-08 | NorthamptonvBuryLeague Two · England | 1–1 | League Two · England |
| 2010-05-08 | DarlingtonvDag and RedLeague Two · England | 0–2 | League Two · England |
| 2010-05-08 | GrossetovPiacenzaSerie B · Italy | 3–3 | Serie B · Italy |
| 2010-05-08 | RecreativovLevanteLa Liga 2 · Spain | 0–1 | La Liga 2 · Spain |
| 2010-05-08 | BrightonvYeovilLeague One · England | 1–0 | League One · England |
| 2010-05-08 | GalatasarayvAntalyasporSüper Lig · Turkey | 1–2 | Süper Lig · Turkey |
| 2010-05-08 | GrenoblevBoulogneLigue 1 · France | 2–0 | Ligue 1 · France |
| 2010-05-08 | ExetervHuddersfieldLeague One · England | 2–1 | League One · England |
| 2010-05-08 | KilmarnockvFalkirkPremiership · Scotland | 0–0 | Premiership · Scotland |
| 2010-05-08 | VallecanovHuescaLa Liga 2 · Spain | 4–1 | La Liga 2 · Spain |
| 2010-05-08 | CesenavPadovaSerie B · Italy | 2–0 | Serie B · Italy |
| 2010-05-08 | JerseyvGuernseyMuratti Vase | 0–1 | Muratti Vase |
| 2010-05-08 | M'gladbachvLeverkusenBundesliga · Germany | 1–1 | Bundesliga · Germany |
| 2010-05-08 | LeiriavPortoPrimeira Liga · Portugal | 1–4 | Primeira Liga · Portugal |
| 2010-05-08 | GetafevMalagaLa Liga · Spain | 2–1 | La Liga · Spain |
| 2010-05-08 | RennesvNiceLigue 1 · France | 2–2 | Ligue 1 · France |
| 2010-05-08 | LeedsvBristol RvsLeague One · England | 2–1 | League One · England |
| 2010-05-08 | HerefordvRotherhamLeague Two · England | 3–0 | League Two · England |
| 2010-05-08 | CeltavGironaLa Liga 2 · Spain | 2–1 | La Liga 2 · Spain |
| 2010-05-08 | St EtiennevToulouseLigue 1 · France | 0–1 | Ligue 1 · France |
| 2010-05-08 | BochumvHannoverBundesliga · Germany | 0–3 | Bundesliga · Germany |
| 2010-05-08 | MontpelliervLorientLigue 1 · France | 2–1 | Ligue 1 · France |
| 2010-05-07 | AngersvNantesLigue 2 · France | 2–0 | Ligue 2 · France |
| 2010-05-07 | BesiktasvManisasporSüper Lig · Turkey | 2–0 | Süper Lig · Turkey |