| 2021-09-21 | AtalantavSassuoloSerie A · Italy | 2–1 | Serie A · Italy |
| 2021-09-21 | ZurichvServetteSuper League · Switzerland | 2–2 | Super League · Switzerland |
| 2021-09-21 | GrenoblevNancyLigue 2 · France | 4–1 | Ligue 2 · France |
| 2021-09-21 | CaenvDijonLigue 2 · France | 0–1 | Ligue 2 · France |
| 2021-09-21 | Paris FCvNimesLigue 2 · France | 2–0 | Ligue 2 · France |
| 2021-09-21 | Dover AthleticvSolihullNational League · England | 0–0 | National League · England |
| 2021-09-21 | AuxerrevRodezLigue 2 · France | 1–0 | Ligue 2 · France |
| 2021-09-21 | For SittardvAjaxEredivisie · Netherlands | 0–5 | Eredivisie · Netherlands |
| 2021-09-21 | PortsmouthvPlymouthLeague One · England | 2–2 | League One · England |
| 2021-09-21 | BolognavGenoaSerie A · Italy | 2–2 | Serie A · Italy |
| 2021-09-21 | AlessandriavAscoliSerie B · Italy | 1–3 | Serie B · Italy |
| 2021-09-21 | WaalwijkvWillem IIEredivisie · Netherlands | 1–2 | Eredivisie · Netherlands |
| 2021-09-21 | Yeni MalatyasporvSivassporSüper Lig · Turkey | 0–1 | Süper Lig · Turkey |
| 2021-09-21 | LevantevCeltaLa Liga · Spain | 0–2 | La Liga · Spain |
| 2021-09-21 | SpalvVicenzaSerie B · Italy | 3–2 | Serie B · Italy |
| 2021-09-21 | CrotonevLecceSerie B · Italy | 0–3 | Serie B · Italy |
| 2021-09-21 | KaragumrukvAntalyasporSüper Lig · Turkey | 0–0 | Süper Lig · Turkey |
| 2021-09-21 | DunkerquevToulouseLigue 2 · France | 0–2 | Ligue 2 · France |
| 2021-09-21 | Ath BilbaovVallecanoLa Liga · Spain | 1–2 | La Liga · Spain |
| 2021-09-21 | BeneventovCittadellaSerie B · Italy | 4–1 | Serie B · Italy |
| 2021-09-21 | CrewevMorecambeLeague One · England | 1–3 | League One · England |
| 2021-09-21 | Defensa y JusticiavBanfieldLiga Profesional · Argentina | 0–0 | Liga Profesional · Argentina |
| 2021-09-21 | ValenciennesvBastiaLigue 2 · France | 2–1 | Ligue 2 · France |
| 2021-09-21 | GetafevAth MadridLa Liga · Spain | 1–2 | La Liga · Spain |
| 2021-09-21 | FiorentinavInterSerie A · Italy | 1–3 | Serie A · Italy |
| 2021-09-21 | CosenzavComoSerie B · Italy | 2–0 | Serie B · Italy |
| 2021-09-21 | RizesporvAltaySüper Lig · Turkey | 1–2 | Süper Lig · Turkey |
| 2021-09-21 | PordenonevRegginaSerie B · Italy | 1–1 | Serie B · Italy |
| 2021-09-21 | AjacciovNiortLigue 2 · France | 0–0 | Ligue 2 · France |
| 2021-09-21 | CuiabavFluminenseSerie A · Brazil | 2–2 | Serie A · Brazil |
| 2021-09-21 | PisavMonzaSerie B · Italy | 2–1 | Serie B · Italy |
| 2021-09-21 | AmiensvPau FCLigue 2 · France | 2–2 | Ligue 2 · France |
| 2021-09-21 | GuingampvSochauxLigue 2 · France | 1–2 | Ligue 2 · France |
| 2021-09-21 | Le HavrevQuevilly RouenLigue 2 · France | 1–0 | Ligue 2 · France |
| 2021-09-20 | SilkeborgvAarhusSuperliga · Denmark | 0–2 | Superliga · Denmark |
| 2021-09-20 | Sp BragavTondelaPrimeira Liga · Portugal | 3–1 | Primeira Liga · Portugal |
| 2021-09-20 | CFR ClujvUniv. CraiovaSuperliga · Romania | 1–0 | Superliga · Romania |
| 2021-09-20 | Portland TimbersvLos Angeles FCMLS · United States | 2–1 | MLS · United States |
| 2021-09-20 | AIKvGoteborgAllsvenskan · Sweden | 3–1 | Allsvenskan · Sweden |
| 2021-09-20 | LanusvNewells Old BoysLiga Profesional · Argentina | 1–2 | Liga Profesional · Argentina |
| 2021-09-20 | HammarbyvVarbergAllsvenskan · Sweden | 1–0 | Allsvenskan · Sweden |
| 2021-09-20 | Flamengo RJvGremioSerie A · Brazil | 0–1 | Serie A · Brazil |
| 2021-09-20 | BarcelonavGranadaLa Liga · Spain | 1–1 | La Liga · Spain |
| 2021-09-20 | CSKA MoscowvSpartak MoscowPremier League · Russia | 1–0 | Premier League · Russia |
| 2021-09-20 | Rubin KazanvZenitPremier League · Russia | 1–3 | Premier League · Russia |
| 2021-09-20 | FrosinonevBresciaSerie B · Italy | 2–2 | Serie B · Italy |
| 2021-09-20 | Gimnasia L.P.vUnion de Santa FeLiga Profesional · Argentina | 1–0 | Liga Profesional · Argentina |
| 2021-09-20 | BenficavBoavistaPrimeira Liga · Portugal | 3–1 | Primeira Liga · Portugal |
| 2021-09-20 | BurgosvLas PalmasLa Liga 2 · Spain | 0–0 | La Liga 2 · Spain |
| 2021-09-20 | Santos LagunavPueblaLiga MX · Mexico | 1–1 | Liga MX · Mexico |