| 2016-09-17 | ExetervPlymouthLeague Two · England | 0–2 | League Two · England |
| 2016-09-17 | Sagan TosuvSanfrecce HiroshimaJ1 League · Japan | 2–3 | J1 League · Japan |
| 2016-09-17 | Din. BucurestivPoli IasiSuperliga · Romania | 3–1 | Superliga · Romania |
| 2016-09-17 | CorinthiansvPalmeirasSerie A · Brazil | 0–2 | Serie A · Brazil |
| 2016-09-17 | ChestervBraintree TownNational League · England | 1–0 | National League · England |
| 2016-09-17 | DoncastervNewport CountyLeague Two · England | 2–0 | League Two · England |
| 2016-09-17 | SilkeborgvAarhusSuperliga · Denmark | 1–0 | Superliga · Denmark |
| 2016-09-17 | BradfordvBristol RvsLeague One · England | 1–1 | League One · England |
| 2016-09-17 | DijonvMetzLigue 1 · France | 0–0 | Ligue 1 · France |
| 2016-09-17 | Man CityvBournemouthPremier League · England | 4–0 | Premier League · England |
| 2016-09-17 | KaiserslauternvStuttgart2. Bundesliga · Germany | 0–1 | 2. Bundesliga · Germany |
| 2016-09-17 | PisavBresciaSerie B · Italy | 1–0 | Serie B · Italy |
| 2016-09-17 | Akhmat GroznyvAmkarPremier League · Russia | 1–3 | Premier League · Russia |
| 2016-09-17 | Wisla PlockvPogon SzczecinEkstraklasa · Poland | 2–0 | Ekstraklasa · Poland |
| 2016-09-17 | Leyton OrientvYeovilLeague Two · England | 0–1 | League Two · England |
| 2016-09-17 | Milton Keynes DonsvOxfordLeague One · England | 0–0 | League One · England |
| 2016-09-17 | Queens ParkvAlloaLeague One · Scotland | 1–2 | League One · Scotland |
| 2016-09-17 | BordeauxvAngersLigue 1 · France | 0–1 | Ligue 1 · France |
| 2016-09-17 | Vissel KobevKashiwa ReysolJ1 League · Japan | 1–1 | J1 League · Japan |
| 2016-09-17 | YorkvDover AthleticNational League · England | 0–1 | National League · England |
| 2016-09-17 | RodavHeerenveenEredivisie · Netherlands | 0–3 | Eredivisie · Netherlands |
| 2016-09-17 | MotherwellvHamiltonPremiership · Scotland | 4–2 | Premiership · Scotland |
| 2016-09-17 | BraunschweigvSandhausen2. Bundesliga · Germany | 2–1 | 2. Bundesliga · Germany |
| 2016-09-17 | Seattle SoundersvVancouver WhitecapsMLS · United States | 1–0 | MLS · United States |
| 2016-09-17 | Shonan BellmarevAvispa FukuokaJ1 League · Japan | 0–2 | J1 League · Japan |
| 2016-09-17 | VeracruzvMonarcasLiga MX · Mexico | 1–3 | Liga MX · Mexico |
| 2016-09-17 | Wolfsberger ACvSt. PoltenBundesliga · Austria | 1–1 | Bundesliga · Austria |
| 2016-09-17 | BirminghamvSheffield WedsChampionship · England | 2–1 | Championship · England |
| 2016-09-17 | ToulousevGuingampLigue 1 · France | 2–1 | Ligue 1 · France |
| 2016-09-17 | SwindonvBuryLeague One · England | 1–2 | League One · England |
| 2016-09-17 | VikingvMoldeEliteserien · Norway | 1–0 | Eliteserien · Norway |
| 2016-09-17 | SpalvVirtus EntellaSerie B · Italy | 2–2 | Serie B · Italy |
| 2016-09-17 | LeganesvBarcelonaLa Liga · Spain | 1–5 | La Liga · Spain |
| 2016-09-17 | M'gladbachvWerder BremenBundesliga · Germany | 4–1 | Bundesliga · Germany |
| 2016-09-17 | WycombevStevenageLeague Two · England | 1–0 | League Two · England |
| 2016-09-17 | Bristol CityvDerbyChampionship · England | 1–1 | Championship · England |
| 2016-09-17 | NancyvNantesLigue 1 · France | 1–1 | Ligue 1 · France |
| 2016-09-17 | San LorenzovVelez SarsfieldLiga Profesional · Argentina | 2–1 | Liga Profesional · Argentina |
| 2016-09-17 | St TruidenvWaasland-BeverenPro League · Belgium | 4–1 | Pro League · Belgium |
| 2016-09-17 | Portland TimbersvPhiladelphia UnionMLS · United States | 2–1 | MLS · United States |
| 2016-09-17 | Rosario CentralvPatronatoLiga Profesional · Argentina | 2–1 | Liga Profesional · Argentina |
| 2016-09-17 | LecznavKorona KielceEkstraklasa · Poland | 4–0 | Ekstraklasa · Poland |
| 2016-09-17 | KalmarvOstersundsAllsvenskan · Sweden | 2–0 | Allsvenskan · Sweden |
| 2016-09-17 | WesterlovMouscronPro League · Belgium | 1–3 | Pro League · Belgium |
| 2016-09-17 | ChesterfieldvNorthamptonLeague One · England | 3–1 | League One · England |
| 2016-09-17 | BarnetvColchesterLeague Two · England | 1–1 | League Two · England |
| 2016-09-17 | HullvArsenalPremier League · England | 1–4 | Premier League · England |
| 2016-09-17 | WiganvFulhamChampionship · England | 0–0 | Championship · England |
| 2016-09-17 | NapolivBolognaSerie A · Italy | 3–1 | Serie A · Italy |
| 2016-09-17 | MacclesfieldvEastleighNational League · England | 0–1 | National League · England |