| 2021-09-25 | StockportvWrexhamNational League · England | 2–1 | National League · England |
| 2021-09-25 | RochdalevOldhamLeague Two · England | 0–1 | League Two · England |
| 2021-09-25 | MorecambevAccringtonLeague One · England | 3–3 | League One · England |
| 2021-09-25 | Sheffield UnitedvDerbyChampionship · England | 1–0 | Championship · England |
| 2021-09-25 | SalfordvNorthamptonLeague Two · England | 2–2 | League Two · England |
| 2021-09-25 | AltrinchamvNotts CountyNational League · England | 1–0 | National League · England |
| 2021-09-25 | BanfieldvAtl. TucumanLiga Profesional · Argentina | 2–0 | Liga Profesional · Argentina |
| 2021-09-25 | ZenitvKrylya SovetovPremier League · Russia | 2–1 | Premier League · Russia |
| 2021-09-25 | IbizavBurgosLa Liga 2 · Spain | 2–0 | La Liga 2 · Spain |
| 2021-09-25 | CearavChapecoense-SCSerie A · Brazil | 1–0 | Serie A · Brazil |
| 2021-09-25 | HeartsvLivingstonPremiership · Scotland | 3–0 | Premiership · Scotland |
| 2021-09-25 | AlavesvAth MadridLa Liga · Spain | 1–0 | La Liga · Spain |
| 2021-09-25 | AmorebietavEibarLa Liga 2 · Spain | 1–1 | La Liga 2 · Spain |
| 2021-09-25 | St EtiennevNiceLigue 1 · France | 0–3 | Ligue 1 · France |
| 2021-09-25 | UtrechtvZwolleEredivisie · Netherlands | 5–1 | Eredivisie · Netherlands |
| 2021-09-25 | MaidenheadvGrimsbyNational League · England | 1–1 | National League · England |
| 2021-09-25 | RotherhamvCreweLeague One · England | 1–1 | League One · England |
| 2021-09-25 | FeyenoordvNijmegenEredivisie · Netherlands | 5–3 | Eredivisie · Netherlands |
| 2021-09-25 | AscolivBresciaSerie B · Italy | 2–3 | Serie B · Italy |
| 2021-09-25 | ValenciavAth BilbaoLa Liga · Spain | 1–1 | La Liga · Spain |
| 2021-09-25 | SwindonvColchesterLeague Two · England | 0–0 | League Two · England |
| 2021-09-25 | DunfermlinevHamiltonChampionship · Scotland | 0–0 | Championship · Scotland |
| 2021-09-25 | Cove RangersvAirdrie UtdLeague One · Scotland | 1–0 | League One · Scotland |
| 2021-09-25 | Vegalta SendaivTokushimaJ1 League · Japan | 0–1 | J1 League · Japan |
| 2021-09-25 | Estudiantes L.P.vPlatenseLiga Profesional · Argentina | 1–1 | Liga Profesional · Argentina |
| 2021-09-25 | AldershotvHalifaxNational League · England | 0–1 | National League · England |
| 2021-09-25 | Crawley TownvBradfordLeague Two · England | 2–1 | League Two · England |
| 2021-09-25 | KarlsruhevSt Pauli2. Bundesliga · Germany | 1–3 | 2. Bundesliga · Germany |
| 2021-09-25 | PaderbornvHolstein Kiel2. Bundesliga · Germany | 1–2 | 2. Bundesliga · Germany |
| 2021-09-25 | MonzavPordenoneSerie B · Italy | 3–1 | Serie B · Italy |
| 2021-09-25 | MotherwellvRoss CountyPremiership · Scotland | 2–1 | Premiership · Scotland |
| 2021-09-25 | IngolstadtvFortuna Dusseldorf2. Bundesliga · Germany | 1–2 | 2. Bundesliga · Germany |
| 2021-09-25 | Philadelphia UnionvAtlanta UtdMLS · United States | 1–0 | MLS · United States |
| 2021-09-25 | GuimaraesvBenficaPrimeira Liga · Portugal | 1–3 | Primeira Liga · Portugal |
| 2021-09-25 | Kelty HeartsvStranraerLeague Two · Scotland | 1–0 | League Two · Scotland |
| 2021-09-25 | WalsallvBristol RvsLeague Two · England | 1–2 | League Two · England |
| 2021-09-25 | Willem IIvPSV EindhovenEredivisie · Netherlands | 2–1 | Eredivisie · Netherlands |
| 2021-09-25 | WiganvCheltenhamLeague One · England | 2–0 | League One · England |
| 2021-09-25 | StromsgodsetvSarpsborg 08Eliteserien · Norway | 5–0 | Eliteserien · Norway |
| 2021-09-24 | DijonvValenciennesLigue 2 · France | 0–1 | Ligue 2 · France |
| 2021-09-24 | DundalkvSligo RoversPremier Division · Ireland | 4–1 | Premier Division · Ireland |
| 2021-09-24 | RodezvGrenobleLigue 2 · France | 1–1 | Ligue 2 · France |
| 2021-09-24 | NimesvLe HavreLigue 2 · France | 0–1 | Ligue 2 · France |
| 2021-09-24 | HeidenheimvDarmstadt2. Bundesliga · Germany | 2–1 | 2. Bundesliga · Germany |
| 2021-09-24 | ArbroathvKilmarnockChampionship · Scotland | 0–0 | Championship · Scotland |
| 2021-09-24 | PachucavNecaxaLiga MX · Mexico | 1–0 | Liga MX · Mexico |
| 2021-09-24 | AltayvBesiktasSüper Lig · Turkey | 2–1 | Süper Lig · Turkey |
| 2021-09-24 | BarrowvNewport CountyLeague Two · England | 2–1 | League Two · England |
| 2021-09-24 | Quevilly RouenvGuingampLigue 2 · France | 2–2 | Ligue 2 · France |
| 2021-09-24 | St. PatricksvShamrock RoversPremier Division · Ireland | 0–1 | Premier Division · Ireland |