| 2021-09-25 | ChesterfieldvTorquayNational League · England | 2–2 | National League · England |
| 2021-09-25 | SwanseavHuddersfieldChampionship · England | 1–0 | Championship · England |
| 2021-09-25 | Inverness CvQueen of SthChampionship · Scotland | 2–1 | Championship · Scotland |
| 2021-09-25 | IntervAtalantaSerie A · Italy | 2–2 | Serie A · Italy |
| 2021-09-25 | Nott'm ForestvMillwallChampionship · England | 1–1 | Championship · England |
| 2021-09-25 | ServettevLausanneSuper League · Switzerland | 1–1 | Super League · Switzerland |
| 2021-09-25 | LeedsvWest HamPremier League · England | 1–2 | Premier League · England |
| 2021-09-25 | Milton Keynes DonsvWycombeLeague One · England | 1–0 | League One · England |
| 2021-09-25 | BlackpoolvBarnsleyChampionship · England | 1–0 | Championship · England |
| 2021-09-25 | StrasbourgvLilleLigue 1 · France | 1–2 | Ligue 1 · France |
| 2021-09-25 | ComovBeneventoSerie B · Italy | 1–1 | Serie B · Italy |
| 2021-09-25 | East FifevClydeLeague One · Scotland | 0–2 | League One · Scotland |
| 2021-09-25 | Dag and RedvSolihullNational League · England | 5–1 | National League · England |
| 2021-09-25 | FC ArgesvChindia TargovisteSuperliga · Romania | 0–0 | Superliga · Romania |
| 2021-09-25 | AltachvA. KlagenfurtBundesliga · Austria | 0–4 | Bundesliga · Austria |
| 2021-09-25 | AldosivivGimnasia L.P.Liga Profesional · Argentina | 1–3 | Liga Profesional · Argentina |
| 2021-09-25 | BurtonvLincolnLeague One · England | 1–2 | League One · England |
| 2021-09-25 | Warta PoznanvZaglebieEkstraklasa · Poland | 0–2 | Ekstraklasa · Poland |
| 2021-09-25 | Hansa RostockvSchalke 042. Bundesliga · Germany | 0–2 | 2. Bundesliga · Germany |
| 2021-09-25 | Kings LynnvWealdstoneNational League · England | 0–1 | National League · England |
| 2021-09-25 | Union BerlinvBielefeldBundesliga · Germany | 1–0 | Bundesliga · Germany |
| 2021-09-25 | BlackburnvCardiffChampionship · England | 5–1 | Championship · England |
| 2021-09-25 | LegiavRakowEkstraklasa · Poland | 2–3 | Ekstraklasa · Poland |
| 2021-09-25 | KhimkivLokomotiv MoscowPremier League · Russia | 0–0 | Premier League · Russia |
| 2021-09-25 | PlymouthvDoncasterLeague One · England | 2–1 | League One · England |
| 2021-09-25 | Wolfsberger ACvSalzburgBundesliga · Austria | 0–2 | Bundesliga · Austria |
| 2021-09-25 | Ein FrankfurtvFC KolnBundesliga · Germany | 1–1 | Bundesliga · Germany |
| 2021-09-25 | DumbartonvAlloaLeague One · Scotland | 1–1 | League One · Scotland |
| 2021-09-25 | Young BoysvSt. GallenSuper League · Switzerland | 2–1 | Super League · Switzerland |
| 2021-09-25 | BarnetvWeymouthNational League · England | 3–1 | National League · England |
| 2021-09-25 | PueblavCruz AzulLiga MX · Mexico | 1–1 | Liga MX · Mexico |
| 2021-09-25 | HarrogatevStevenageLeague Two · England | 0–0 | League Two · England |
| 2021-09-25 | ReadingvMiddlesbroughChampionship · England | 1–0 | Championship · England |
| 2021-09-25 | Yokohama FCvYokohama F. MarinosJ1 League · Japan | 2–2 | J1 League · Japan |
| 2021-09-25 | Dover AthleticvBromleyNational League · England | 0–1 | National League · England |
| 2021-09-25 | Club TijuanavMazatlan FCLiga MX · Mexico | 0–0 | Liga MX · Mexico |
| 2021-09-25 | BournemouthvLutonChampionship · England | 2–1 | Championship · England |
| 2021-09-25 | LyonvLorientLigue 1 · France | 1–1 | Ligue 1 · France |
| 2021-09-25 | Ad. DemirsporvGaziantepSüper Lig · Turkey | 4–0 | Süper Lig · Turkey |
| 2021-09-25 | Spartak MoscowvUfaPremier League · Russia | 2–0 | Premier League · Russia |
| 2021-09-25 | SpeziavMilanSerie A · Italy | 1–2 | Serie A · Italy |
| 2021-09-25 | HartlepoolvExeterLeague Two · England | 1–1 | League Two · England |
| 2021-09-25 | BastiavAjaccioLigue 2 · France | 2–0 | Ligue 2 · France |
| 2021-09-25 | ChelseavMan CityPremier League · England | 0–1 | Premier League · England |
| 2021-09-25 | TondelavFamalicaoPrimeira Liga · Portugal | 3–2 | Primeira Liga · Portugal |
| 2021-09-25 | Man UnitedvAston VillaPremier League · England | 0–1 | Premier League · England |
| 2021-09-25 | CharltonvPortsmouthLeague One · England | 2–2 | League One · England |
| 2021-09-25 | CambridgevFleetwood TownLeague One · England | 2–2 | League One · England |
| 2021-09-25 | VitessevFor SittardEredivisie · Netherlands | 1–1 | Eredivisie · Netherlands |
| 2021-09-25 | Las PalmasvPonferradinaLa Liga 2 · Spain | 2–1 | La Liga 2 · Spain |