| 2021-09-25 | Arsenal SarandivLanusLiga Profesional · Argentina | 1–0 | Liga Profesional · Argentina |
| 2021-09-25 | SuttonvCarlisleLeague Two · England | 4–0 | League Two · England |
| 2021-09-25 | Real MadridvVillarrealLa Liga · Spain | 0–0 | La Liga · Spain |
| 2021-09-25 | StandardvSt TruidenPro League · Belgium | 1–2 | Pro League · Belgium |
| 2021-09-25 | EastleighvWokingNational League · England | 3–2 | National League · England |
| 2021-09-25 | Leyton OrientvMansfieldLeague Two · England | 0–0 | League Two · England |
| 2021-09-25 | IpswichvSheffield WedsLeague One · England | 1–1 | League One · England |
| 2021-09-25 | CittadellavLecceSerie B · Italy | 1–2 | Serie B · Italy |
| 2021-09-25 | WatfordvNewcastlePremier League · England | 1–1 | Premier League · England |
| 2021-09-25 | AntalyasporvYeni MalatyasporSüper Lig · Turkey | 1–0 | Süper Lig · Turkey |
| 2021-09-25 | SunderlandvBoltonLeague One · England | 1–0 | League One · England |
| 2021-09-25 | Avispa FukuokavSagan TosuJ1 League · Japan | 3–0 | J1 League · Japan |
| 2021-09-25 | RegginavFrosinoneSerie B · Italy | 0–0 | Serie B · Italy |
| 2021-09-25 | LillestromvTromsoEliteserien · Norway | 1–2 | Eliteserien · Norway |
| 2021-09-25 | SevillavEspanolLa Liga · Spain | 2–0 | La Liga · Spain |
| 2021-09-25 | FC TokyovUrawa RedsJ1 League · Japan | 1–2 | J1 League · Japan |
| 2021-09-25 | StirlingvCowdenbeathLeague Two · Scotland | 4–0 | League Two · Scotland |
| 2021-09-25 | FCSBvAcademica ClinceniSuperliga · Romania | 3–2 | Superliga · Romania |
| 2021-09-25 | BirminghamvPrestonChampionship · England | 0–0 | Championship · England |
| 2021-09-25 | MontrosevFalkirkLeague One · Scotland | 2–2 | League One · Scotland |
| 2021-09-25 | Forest GreenvTranmereLeague Two · England | 0–0 | League Two · England |
| 2021-09-25 | Malmo FFvOrebroAllsvenskan · Sweden | 5–1 | Allsvenskan · Sweden |
| 2021-09-25 | DundeevRangersPremiership · Scotland | 0–1 | Premiership · Scotland |
| 2021-09-25 | OxfordvGillinghamLeague One · England | 1–1 | League One · England |
| 2021-09-25 | AtlasvClub LeonLiga MX · Mexico | 2–0 | Liga MX · Mexico |
| 2021-09-25 | GenoavVeronaSerie A · Italy | 3–3 | Serie A · Italy |
| 2021-09-25 | TirolvRiedBundesliga · Austria | 4–2 | Bundesliga · Austria |
| 2021-09-25 | RB LeipzigvHerthaBundesliga · Germany | 6–0 | Bundesliga · Germany |
| 2021-09-25 | Bristol CityvFulhamChampionship · England | 1–1 | Championship · England |
| 2021-09-25 | Annan AthleticvEdinburgh CityLeague Two · Scotland | 1–3 | League Two · Scotland |
| 2021-09-25 | ScunthorpevPort ValeLeague Two · England | 0–1 | League Two · England |
| 2021-09-25 | Boreham WoodvYeovilNational League · England | 2–1 | National League · England |
| 2021-09-25 | BrentfordvLiverpoolPremier League · England | 3–3 | Premier League · England |
| 2021-09-25 | M'gladbachvDortmundBundesliga · Germany | 1–0 | Bundesliga · Germany |
| 2021-09-25 | MoreirensevAroucaPrimeira Liga · Portugal | 2–1 | Primeira Liga · Portugal |
| 2021-09-25 | Talleres CordobavRosario CentralLiga Profesional · Argentina | 4–1 | Liga Profesional · Argentina |
| 2021-09-25 | CorinthiansvPalmeirasSerie A · Brazil | 2–1 | Serie A · Brazil |
| 2021-09-25 | AjaxvGroningenEredivisie · Netherlands | 3–0 | Eredivisie · Netherlands |
| 2021-09-25 | ForfarvAlbion RvsLeague Two · Scotland | 3–1 | League Two · Scotland |
| 2021-09-25 | Stal MielecvRadomiak RadomEkstraklasa · Poland | 1–0 | Ekstraklasa · Poland |
| 2021-09-25 | AyrvMortonChampionship · Scotland | 0–0 | Championship · Scotland |
| 2021-09-25 | EvertonvNorwichPremier League · England | 2–0 | Premier League · England |
| 2021-09-25 | HoffenheimvWolfsburgBundesliga · Germany | 3–1 | Bundesliga · Germany |
| 2021-09-25 | LeverkusenvMainzBundesliga · Germany | 1–0 | Bundesliga · Germany |
| 2021-09-25 | ShrewsburyvAFC WimbledonLeague One · England | 2–1 | League One · England |
| 2021-09-25 | SivassporvKaragumrukSüper Lig · Turkey | 4–0 | Süper Lig · Turkey |
| 2021-09-25 | Queens ParkvPeterheadLeague One · Scotland | 3–2 | League One · Scotland |
| 2021-09-25 | StokevHullChampionship · England | 2–0 | Championship · England |
| 2021-09-25 | Beerschot VAvEupenPro League · Belgium | 0–3 | Pro League · Belgium |
| 2021-09-25 | LeicestervBurnleyPremier League · England | 2–2 | Premier League · England |