| 2016-09-24 | PlymouthvHartlepoolLeague Two · England | 1–1 | League Two · England |
| 2016-09-24 | Olimpo Bahia BlancavGodoy CruzLiga Profesional · Argentina | 3–0 | Liga Profesional · Argentina |
| 2016-09-24 | LutonvDoncasterLeague Two · England | 3–1 | League Two · England |
| 2016-09-24 | YeovilvCheltenhamLeague Two · England | 4–2 | League Two · England |
| 2016-09-24 | Sp GijonvBarcelonaLa Liga · Spain | 0–5 | La Liga · Spain |
| 2016-09-24 | MansfieldvGrimsbyLeague Two · England | 0–1 | League Two · England |
| 2016-09-24 | Ath BilbaovSevillaLa Liga · Spain | 3–1 | La Liga · Spain |
| 2016-09-24 | St. GallenvBaselSuper League · Switzerland | 1–3 | Super League · Switzerland |
| 2016-09-24 | PanetolikosvPanathinaikosSuper League · Greece | 0–0 | Super League · Greece |
| 2016-09-24 | HeartsvRoss CountyPremiership · Scotland | 0–0 | Premiership · Scotland |
| 2016-09-24 | Sp BragavSetubalPrimeira Liga · Portugal | 2–1 | Primeira Liga · Portugal |
| 2016-09-24 | U CraiovavFC BotosaniSuperliga · Romania | 1–0 | Superliga · Romania |
| 2016-09-24 | PortsmouthvBarnetLeague Two · England | 5–1 | League Two · England |
| 2016-09-24 | Atl. RafaelavRosario CentralLiga Profesional · Argentina | 0–0 | Liga Profesional · Argentina |
| 2016-09-24 | Dover AthleticvLincolnNational League · England | 2–0 | National League · England |
| 2016-09-24 | Inverness CvDundeePremiership · Scotland | 3–1 | Premiership · Scotland |
| 2016-09-24 | PalmeirasvCoritibaSerie A · Brazil | 2–1 | Serie A · Brazil |
| 2016-09-24 | Sturm GrazvWolfsberger ACBundesliga · Austria | 3–0 | Bundesliga · Austria |
| 2016-09-24 | DerbyvBlackburnChampionship · England | 1–2 | Championship · England |
| 2016-09-24 | AugsburgvDarmstadtBundesliga · Germany | 1–0 | Bundesliga · Germany |
| 2016-09-24 | GroningenvHeraclesEredivisie · Netherlands | 0–0 | Eredivisie · Netherlands |
| 2016-09-24 | MainzvLeverkusenBundesliga · Germany | 2–3 | Bundesliga · Germany |
| 2016-09-24 | Raith RvsvDumbartonChampionship · Scotland | 3–2 | Championship · Scotland |
| 2016-09-24 | BastiavGuingampLigue 1 · France | 1–0 | Ligue 1 · France |
| 2016-09-24 | StokevWest BromPremier League · England | 1–1 | Premier League · England |
| 2016-09-24 | Queens ParkvStranraerLeague One · Scotland | 0–2 | League One · Scotland |
| 2016-09-24 | AjaxvZwolleEredivisie · Netherlands | 5–1 | Eredivisie · Netherlands |
| 2016-09-24 | LuzernvLausanneSuper League · Switzerland | 1–3 | Super League · Switzerland |
| 2016-09-24 | PartickvMotherwellPremiership · Scotland | 1–1 | Premiership · Scotland |
| 2016-09-24 | EibarvSociedadLa Liga · Spain | 2–0 | La Liga · Spain |
| 2016-09-24 |  MonacovAngersLigue 1 · France | 2–1 | Ligue 1 · France |
| 2016-09-24 | AZ AlkmaarvGo Ahead EaglesEredivisie · Netherlands | 2–2 | Eredivisie · Netherlands |
| 2016-09-24 | ChiapasvNecaxaLiga MX · Mexico | 2–2 | Liga MX · Mexico |
| 2016-09-24 | Dundee UnitedvMortonChampionship · Scotland | 2–1 | Championship · Scotland |
| 2016-09-24 | Sheffield WedsvNott'm ForestChampionship · England | 2–1 | Championship · England |
| 2016-09-24 | Din. BucurestivConcordiaSuperliga · Romania | 0–1 | Superliga · Romania |
| 2016-09-24 | QPRvBirminghamChampionship · England | 1–1 | Championship · England |
| 2016-09-24 | StirlingvElginLeague Two · Scotland | 0–4 | League Two · Scotland |
| 2016-09-24 | St. PoltenvSK RapidBundesliga · Austria | 1–1 | Bundesliga · Austria |
| 2016-09-24 | PanioniosvVeriaSuper League · Greece | 1–2 | Super League · Greece |
| 2016-09-24 | BrightonvBarnsleyChampionship · England | 2–0 | Championship · England |
| 2016-09-24 | LatinavTernanaSerie B · Italy | 1–1 | Serie B · Italy |
| 2016-09-24 | GillinghamvCoventryLeague One · England | 2–1 | League One · England |
| 2016-09-24 | MontpelliervMetzLigue 1 · France | 0–1 | Ligue 1 · France |
| 2016-09-24 | AldershotvGatesheadNational League · England | 3–0 | National League · England |
| 2016-09-24 | CordobavGimnasticLa Liga 2 · Spain | 2–0 | La Liga 2 · Spain |
| 2016-09-24 | Pro VercellivCesenaSerie B · Italy | 1–0 | Serie B · Italy |
| 2016-09-24 | ExcelsiorvPSV EindhovenEredivisie · Netherlands | 1–3 | Eredivisie · Netherlands |
| 2016-09-24 | BournemouthvEvertonPremier League · England | 1–0 | Premier League · England |
| 2016-09-24 | Lechia GdanskvRuchEkstraklasa · Poland | 2–1 | Ekstraklasa · Poland |