| 2016-09-24 | FC CopenhagenvAarhusSuperliga · Denmark | 2–0 | Superliga · Denmark |
| 2016-09-24 | RotherhamvCardiffChampionship · England | 1–2 | Championship · England |
| 2016-09-24 | MoldevRosenborgEliteserien · Norway | 1–3 | Eliteserien · Norway |
| 2016-09-24 | SwanseavMan CityPremier League · England | 1–3 | Premier League · England |
| 2016-09-24 | OldhamvSwindonLeague One · England | 0–2 | League One · England |
| 2016-09-24 | DijonvRennesLigue 1 · France | 3–0 | Ligue 1 · France |
| 2016-09-24 | SolihullvBoreham WoodNational League · England | 1–1 | National League · England |
| 2016-09-24 | SunderlandvCrystal PalacePremier League · England | 2–3 | Premier League · England |
| 2016-09-24 | North FerribyvSouthportNational League · England | 0–1 | National League · England |
| 2016-09-24 | PisavAscoliSerie B · Italy | 2–1 | Serie B · Italy |
| 2016-09-24 | VeracruzvGuadalajara ChivasLiga MX · Mexico | 0–1 | Liga MX · Mexico |
| 2016-09-24 | ColchestervAccringtonLeague Two · England | 1–2 | League Two · England |
| 2016-09-24 | ArsenalvChelseaPremier League · England | 3–0 | Premier League · England |
| 2016-09-24 | CharleroivOostendePro League · Belgium | 2–1 | Pro League · Belgium |
| 2016-09-24 | MontrosevBerwickLeague Two · Scotland | 0–0 | League Two · Scotland |
| 2016-09-24 | BesiktasvGalatasaraySüper Lig · Turkey | 2–2 | Süper Lig · Turkey |
| 2016-09-24 | VicenzavAvellinoSerie B · Italy | 0–0 | Serie B · Italy |
| 2016-09-24 | ClydevForfarLeague Two · Scotland | 0–1 | League Two · Scotland |
| 2016-09-24 | AlmeriavLugoLa Liga 2 · Spain | 0–0 | La Liga 2 · Spain |
| 2016-09-24 | WolvesvBrentfordChampionship · England | 3–1 | Championship · England |
| 2016-09-24 | WrexhamvChesterNational League · England | 0–0 | National League · England |
| 2016-09-24 | Fleetwood TownvMilton Keynes DonsLeague One · England | 1–4 | League One · England |
| 2016-09-24 | Toronto FCvPhiladelphia UnionMLS · United States | 1–1 | MLS · United States |
| 2016-09-24 | HeidenheimvKaiserslautern2. Bundesliga · Germany | 3–0 | 2. Bundesliga · Germany |
| 2016-09-24 | MechelenvWaregemPro League · Belgium | 2–3 | Pro League · Belgium |
| 2016-09-24 | NorthamptonvSouthendLeague One · England | 4–0 | League One · England |
| 2016-09-24 | HamburgvBayern MunichBundesliga · Germany | 0–1 | Bundesliga · Germany |
| 2016-09-24 | BordeauxvCaenLigue 1 · France | 0–0 | Ligue 1 · France |
| 2016-09-24 | Newport CountyvCambridgeLeague Two · England | 1–2 | League Two · England |
| 2016-09-24 | BuryvChesterfieldLeague One · England | 2–1 | League One · England |
| 2016-09-24 | LiaoningvYanbianSuper League · China | 3–0 | Super League · China |
| 2016-09-24 | M'gladbachvIngolstadtBundesliga · Germany | 2–0 | Bundesliga · Germany |
| 2016-09-24 | SalernitanavTrapaniSerie B · Italy | 2–0 | Serie B · Italy |
| 2016-09-24 | SpeziavNovaraSerie B · Italy | 1–0 | Serie B · Italy |
| 2016-09-24 | ValenciennesvLensLigue 2 · France | 1–2 | Ligue 2 · France |
| 2016-09-24 | Aston VillavNewcastleChampionship · England | 1–1 | Championship · England |
| 2016-09-24 | FalkirkvAyrChampionship · Scotland | 2–0 | Championship · Scotland |
| 2016-09-24 | Airdrie UtdvAlbion RvsLeague One · Scotland | 0–2 | League One · Scotland |
| 2016-09-24 | GuiseleyvMacclesfieldNational League · England | 1–2 | National League · England |
| 2016-09-24 | TrabzonsporvKonyasporSüper Lig · Turkey | 1–0 | Süper Lig · Turkey |
| 2016-09-24 | TorquayvMaidstoneNational League · England | 2–3 | National League · England |
| 2016-09-24 | MattersburgvRiedBundesliga · Austria | 1–1 | Bundesliga · Austria |
| 2016-09-24 | PeterborovWalsallLeague One · England | 1–1 | League One · England |
| 2016-09-24 | MiddlesbroughvTottenhamPremier League · England | 1–2 | Premier League · England |
| 2016-09-24 | Notts CountyvLeyton OrientLeague Two · England | 3–1 | League Two · England |
| 2016-09-24 | CarpivVirtus EntellaSerie B · Italy | 2–1 | Serie B · Italy |
| 2016-09-24 | CelticvKilmarnockPremiership · Scotland | 6–1 | Premiership · Scotland |
| 2016-09-24 | Braintree TownvForest GreenNational League · England | 0–1 | National League · England |
| 2016-09-24 | JonkopingsvKalmarAllsvenskan · Sweden | 0–1 | Allsvenskan · Sweden |
| 2016-09-24 | Annan AthleticvEdinburgh CityLeague Two · Scotland | 1–1 | League Two · Scotland |