| 2016-09-25 | Club AmericavUNAM PumasLiga MX · Mexico | 2–1 | Liga MX · Mexico |
| 2016-09-25 | CorinthiansvFluminenseSerie A · Brazil | 0–1 | Serie A · Brazil |
| 2016-09-25 | FiorentinavMilanSerie A · Italy | 0–0 | Serie A · Italy |
| 2016-09-25 | EspanolvCeltaLa Liga · Spain | 0–2 | La Liga · Spain |
| 2016-09-25 | Reus DeportiuvVallecanoLa Liga 2 · Spain | 1–1 | La Liga 2 · Spain |
| 2016-09-25 | Atletico-MGvInternacionalSerie A · Brazil | 3–1 | Serie A · Brazil |
| 2016-09-25 | Los Angeles GalaxyvSeattle SoundersMLS · United States | 2–4 | MLS · United States |
| 2016-09-25 | Athletico-PRvPonte PretaSerie A · Brazil | 3–0 | Serie A · Brazil |
| 2016-09-25 | GremiovChapecoense-SCSerie A · Brazil | 1–0 | Serie A · Brazil |
| 2016-09-25 | Pacos FerreiravRio AvePrimeira Liga · Portugal | 2–1 | Primeira Liga · Portugal |
| 2016-09-25 | UtrechtvSparta RotterdamEredivisie · Netherlands | 2–0 | Eredivisie · Netherlands |
| 2016-09-25 | FC VoluntarivFCSBSuperliga · Romania | 2–3 | Superliga · Romania |
| 2016-09-25 | TwentevVitesseEredivisie · Netherlands | 2–1 | Eredivisie · Netherlands |
| 2016-09-25 | NumanciavZaragozaLa Liga 2 · Spain | 2–1 | La Liga 2 · Spain |
| 2016-09-25 | FK Anzi MakhackalavZenitPremier League · Russia | 2–2 | Premier League · Russia |
| 2016-09-25 | AtromitosvAsteras TripolisSuper League · Greece | 1–0 | Super League · Greece |
| 2016-09-25 | AberdeenvRangersPremiership · Scotland | 2–1 | Premiership · Scotland |
| 2016-09-25 | GenoavPescaraSerie A · Italy | 1–1 | Serie A · Italy |
| 2016-09-25 | MonarcasvPueblaLiga MX · Mexico | 2–3 | Liga MX · Mexico |
| 2016-09-25 | Vancouver WhitecapsvColorado RapidsMLS · United States | 3–3 | MLS · United States |
| 2016-09-25 | LanusvAldosiviLiga Profesional · Argentina | 0–0 | Liga Profesional · Argentina |
| 2016-09-25 | Ath MadridvLa CorunaLa Liga · Spain | 1–0 | La Liga · Spain |
| 2016-09-24 | Newells Old BoysvAtl. TucumanLiga Profesional · Argentina | 3–1 | Liga Profesional · Argentina |
| 2016-09-24 | OxfordvCharltonLeague One · England | 1–1 | League One · England |
| 2016-09-24 | ArbroathvCowdenbeathLeague Two · Scotland | 0–0 | League Two · Scotland |
| 2016-09-24 | MorecambevCrawley TownLeague Two · England | 2–3 | League Two · England |
| 2016-09-24 | ZaglebievCracoviaEkstraklasa · Poland | 1–1 | Ekstraklasa · Poland |
| 2016-09-24 | Bristol RvsvPort ValeLeague One · England | 2–1 | League One · England |
| 2016-09-24 | GentvSt TruidenPro League · Belgium | 2–1 | Pro League · Belgium |
| 2016-09-24 | CittadellavBresciaSerie B · Italy | 0–3 | Serie B · Italy |
| 2016-09-24 | Shanghai SIPGvGuangzhou EvergrandeSuper League · China | 0–0 | Super League · China |
| 2016-09-24 | KarlsruhevErzgebirge Aue2. Bundesliga · Germany | 2–0 | 2. Bundesliga · Germany |
| 2016-09-24 | BarivBeneventoSerie B · Italy | 0–4 | Serie B · Italy |
| 2016-09-24 | Velez SarsfieldvRacing ClubLiga Profesional · Argentina | 0–3 | Liga Profesional · Argentina |
| 2016-09-24 | FK RostovvLokomotiv MoscowPremier League · Russia | 1–0 | Premier League · Russia |
| 2016-09-24 | StevenagevExeterLeague Two · England | 0–2 | League Two · England |
| 2016-09-24 | Queen of SthvHibernianChampionship · Scotland | 0–0 | Championship · Scotland |
| 2016-09-24 | Sevilla BvAlcorconLa Liga 2 · Spain | 1–1 | La Liga 2 · Spain |
| 2016-09-24 | Ein FrankfurtvHerthaBundesliga · Germany | 3–3 | Bundesliga · Germany |
| 2016-09-24 | NapolivChievoSerie A · Italy | 2–0 | Serie A · Italy |
| 2016-09-24 | AFC WimbledonvShrewsburyLeague One · England | 1–1 | League One · England |
| 2016-09-24 | MillwallvRochdaleLeague One · England | 2–3 | League One · England |
| 2016-09-24 | ChavesvBenficaPrimeira Liga · Portugal | 0–2 | Primeira Liga · Portugal |
| 2016-09-24 | ElchevLevanteLa Liga 2 · Spain | 0–1 | La Liga 2 · Spain |
| 2016-09-24 | Austria ViennavAdmiraBundesliga · Austria | 1–2 | Bundesliga · Austria |
| 2016-09-24 | NorwichvBurtonChampionship · England | 3–1 | Championship · England |
| 2016-09-24 | Man UnitedvLeicesterPremier League · England | 4–1 | Premier League · England |
| 2016-09-24 | GianninavPlataniasSuper League · Greece | 0–0 | Super League · Greece |
| 2016-09-24 | LiverpoolvHullPremier League · England | 5–1 | Premier League · England |
| 2016-09-24 | LorientvLyonLigue 1 · France | 1–0 | Ligue 1 · France |