| 2016-09-27 | BrentfordvReadingChampionship · England | 4–1 | Championship · England |
| 2016-09-27 | CharltonvOldhamLeague One · England | 1–1 | League One · England |
| 2016-09-27 | CambridgevYeovilLeague Two · England | 1–0 | League Two · England |
| 2016-09-27 | AscolivCesenaSerie B · Italy | 0–0 | Serie B · Italy |
| 2016-09-27 | San Martin S.J.vColon Santa FELiga Profesional · Argentina | 0–0 | Liga Profesional · Argentina |
| 2016-09-27 | BurtonvQPRChampionship · England | 1–1 | Championship · England |
| 2016-09-27 | SouthendvOxfordLeague One · England | 2–1 | League One · England |
| 2016-09-27 | Sheffield UnitedvBristol RvsLeague One · England | 1–0 | League One · England |
| 2016-09-27 | HartlepoolvLutonLeague Two · England | 1–1 | League Two · England |
| 2016-09-27 | Crawley TownvColchesterLeague Two · England | 1–1 | League Two · England |
| 2016-09-27 | Milton Keynes DonsvBuryLeague One · England | 1–3 | League One · England |
| 2016-09-27 | BirminghamvPrestonChampionship · England | 2–2 | Championship · England |
| 2016-09-27 | RochdalevBoltonLeague One · England | 1–0 | League One · England |
| 2016-09-27 | DoncastervCarlisleLeague Two · England | 2–2 | League Two · England |
| 2016-09-27 | IpswichvBrightonChampionship · England | 0–0 | Championship · England |
| 2016-09-27 | SwindonvNorthamptonLeague One · England | 1–3 | League One · England |
| 2016-09-27 | WalsallvScunthorpeLeague One · England | 1–4 | League One · England |
| 2016-09-27 | Ajaccio GFCOvAmiensLigue 2 · France | 1–1 | Ligue 2 · France |
| 2016-09-27 | Port ValevMillwallLeague One · England | 3–1 | League One · England |
| 2016-09-27 | BlackpoolvPortsmouthLeague Two · England | 3–1 | League Two · England |
| 2016-09-27 | Bristol CityvLeedsChampionship · England | 1–0 | Championship · England |
| 2016-09-27 | WiganvWolvesChampionship · England | 2–1 | Championship · England |
| 2016-09-27 | ChesterfieldvGillinghamLeague One · England | 3–3 | League One · England |
| 2016-09-27 | ShrewsburyvPeterboroLeague One · England | 1–1 | League One · England |
| 2016-09-27 | CardiffvDerbyChampionship · England | 0–2 | Championship · England |
| 2016-09-27 | GrimsbyvNewport CountyLeague Two · England | 1–0 | League Two · England |
| 2016-09-27 | St. PatricksvSligo RoversPremier Division · Ireland | 0–0 | Premier Division · Ireland |
| 2016-09-27 | CheltenhamvStevenageLeague Two · England | 0–0 | League Two · England |
| 2016-09-27 | HuddersfieldvRotherhamChampionship · England | 2–1 | Championship · England |
| 2016-09-26 | Santos LagunavClub TijuanaLiga MX · Mexico | 1–2 | Liga MX · Mexico |
| 2016-09-26 | SundsvallvHackenAllsvenskan · Sweden | 0–0 | Allsvenskan · Sweden |
| 2016-09-26 | BurnleyvWatfordPremier League · England | 2–0 | Premier League · England |
| 2016-09-26 | Arsenal SarandivGimnasia L.P.Liga Profesional · Argentina | 2–2 | Liga Profesional · Argentina |
| 2016-09-26 | SilkeborgvAalborgSuperliga · Denmark | 5–3 | Superliga · Denmark |
| 2016-09-26 | CFR ClujvGaz Metan MediasSuperliga · Romania | 1–1 | Superliga · Romania |
| 2016-09-26 | CagliarivSampdoriaSerie A · Italy | 2–1 | Serie A · Italy |
| 2016-09-26 | Inter TurkuvVPSVeikkausliiga · Finland | 1–1 | Veikkausliiga · Finland |
| 2016-09-26 | Columbus CrewvNew England RevolutionMLS · United States | 2–0 | MLS · United States |
| 2016-09-26 | NorrkopingvDjurgardenAllsvenskan · Sweden | 1–3 | Allsvenskan · Sweden |
| 2016-09-26 | StabaekvBodo/GlimtEliteserien · Norway | 1–0 | Eliteserien · Norway |
| 2016-09-26 | Piast GliwicevSlask WroclawEkstraklasa · Poland | 1–1 | Ekstraklasa · Poland |
| 2016-09-26 | OrebrovGefleAllsvenskan · Sweden | 2–2 | Allsvenskan · Sweden |
| 2016-09-26 | GoteborgvOstersundsAllsvenskan · Sweden | 2–0 | Allsvenskan · Sweden |
| 2016-09-26 | Union BerlinvSt Pauli2. Bundesliga · Germany | 2–0 | 2. Bundesliga · Germany |
| 2016-09-26 | AdanasporvAlanyasporSüper Lig · Turkey | 3–2 | Süper Lig · Turkey |
| 2016-09-26 | BelenensesvAroucaPrimeira Liga · Portugal | 1–1 | Primeira Liga · Portugal |
| 2016-09-26 | CrotonevAtalantaSerie A · Italy | 1–3 | Serie A · Italy |
| 2016-09-26 | AlavesvGranadaLa Liga · Spain | 3–1 | La Liga · Spain |
| 2016-09-26 | AstravPoli IasiSuperliga · Romania | 1–0 | Superliga · Romania |
| 2016-09-26 | BrestvReimsLigue 2 · France | 2–1 | Ligue 2 · France |