| 2016-10-01 | PrestonvAston VillaChampionship · England | 2–0 | Championship · England |
| 2016-10-01 | SpalvSalernitanaSerie B · Italy | 3–2 | Serie B · Italy |
| 2016-10-01 | CharltonvRochdaleLeague One · England | 0–1 | League One · England |
| 2016-10-01 | AFC WimbledonvGillinghamLeague One · England | 2–0 | League One · England |
| 2016-10-01 | KilmarnockvAberdeenPremiership · Scotland | 0–4 | Premiership · Scotland |
| 2016-10-01 | Boreham WoodvWrexhamNational League · England | 0–1 | National League · England |
| 2016-10-01 | Rio AvevEstorilPrimeira Liga · Portugal | 1–2 | Primeira Liga · Portugal |
| 2016-10-01 | WolvesvNorwichChampionship · England | 1–2 | Championship · England |
| 2016-10-01 | ShrewsburyvSwindonLeague One · England | 1–1 | League One · England |
| 2016-10-01 | BeneventovNovaraSerie B · Italy | 1–0 | Serie B · Italy |
| 2016-10-01 | Ross CountyvSt JohnstonePremiership · Scotland | 0–2 | Premiership · Scotland |
| 2016-10-01 | ChesterfieldvBradfordLeague One · England | 0–1 | League One · England |
| 2016-10-01 | CowdenbeathvStirlingLeague Two · Scotland | 0–2 | League Two · Scotland |
| 2016-10-01 | Nagoya GrampusvAvispa FukuokaJ1 League · Japan | 5–0 | J1 League · Japan |
| 2016-10-01 | OldhamvMilton Keynes DonsLeague One · England | 0–2 | League One · England |
| 2016-10-01 | VitessevGroningenEredivisie · Netherlands | 2–1 | Eredivisie · Netherlands |
| 2016-10-01 | DumbartonvQueen of SthChampionship · Scotland | 0–0 | Championship · Scotland |
| 2016-10-01 | BirminghamvBlackburnChampionship · England | 1–0 | Championship · England |
| 2016-10-01 | HackenvMalmo FFAllsvenskan · Sweden | 2–4 | Allsvenskan · Sweden |
| 2016-10-01 | TernanavVeronaSerie B · Italy | 0–3 | Serie B · Italy |
| 2016-10-01 | Sanfrecce HiroshimavFC TokyoJ1 League · Japan | 0–1 | J1 League · Japan |
| 2016-10-01 | Gaz Metan MediasvFC VoluntariSuperliga · Romania | 1–0 | Superliga · Romania |
| 2016-10-01 | PS KemivSJKVeikkausliiga · Finland | 1–3 | Veikkausliiga · Finland |
| 2016-10-01 | WatfordvBournemouthPremier League · England | 2–2 | Premier League · England |
| 2016-10-01 | WycombevExeterLeague Two · England | 1–0 | League Two · England |
| 2016-10-01 | FluminensevSport RecifeSerie A · Brazil | 3–1 | Serie A · Brazil |
| 2016-10-01 | HorsensvEsbjergSuperliga · Denmark | 1–0 | Superliga · Denmark |
| 2016-10-01 | BerwickvClydeLeague Two · Scotland | 1–1 | League Two · Scotland |
| 2016-10-01 | AdmiravMattersburgBundesliga · Austria | 1–0 | Bundesliga · Austria |
| 2016-10-01 | FulhamvQPRChampionship · England | 1–2 | Championship · England |
| 2016-10-01 | LeverkusenvDortmundBundesliga · Germany | 2–0 | Bundesliga · Germany |
| 2016-10-01 | CrewevMansfieldLeague Two · England | 1–1 | League Two · England |
| 2016-10-01 | PortsmouthvDoncasterLeague Two · England | 1–2 | League Two · England |
| 2016-10-01 | Racing ClubvPatronatoLiga Profesional · Argentina | 2–0 | Liga Profesional · Argentina |
| 2016-10-01 | Edinburgh CityvArbroathLeague Two · Scotland | 3–3 | League Two · Scotland |
| 2016-10-01 | MaidstonevSolihullNational League · England | 2–4 | National League · England |
| 2016-10-01 | Crawley TownvBlackpoolLeague Two · England | 1–0 | League Two · England |
| 2016-10-01 | BarnetvLeyton OrientLeague Two · England | 0–0 | League Two · England |
| 2016-10-01 | Paris SGvBordeauxLigue 1 · France | 2–0 | Ligue 1 · France |
| 2016-10-01 | Albion RvsvQueens ParkLeague One · Scotland | 2–0 | League One · Scotland |
| 2016-10-01 | PlymouthvYeovilLeague Two · England | 4–1 | League Two · England |
| 2016-10-01 | IngolstadtvHoffenheimBundesliga · Germany | 1–2 | Bundesliga · Germany |
| 2016-10-01 | SouthendvPeterboroLeague One · England | 1–1 | League One · England |
| 2016-10-01 | ZaragozavCordobaLa Liga 2 · Spain | 1–1 | La Liga 2 · Spain |
| 2016-10-01 | Forest GreenvBarrowNational League · England | 0–0 | National League · England |
| 2016-10-01 | RodavNijmegenEredivisie · Netherlands | 0–1 | Eredivisie · Netherlands |
| 2016-10-01 | GimnasticvSevilla BLa Liga 2 · Spain | 1–1 | La Liga 2 · Spain |
| 2016-10-01 | RangersvPartickPremiership · Scotland | 2–0 | Premiership · Scotland |
| 2016-10-01 | SuttonvGuiseleyNational League · England | 1–0 | National League · England |
| 2016-10-01 | Sheffield WedsvBrightonChampionship · England | 1–2 | Championship · England |