| 2009-08-29 | SalisburyvEastbourne BoroughNational League · England | 1–1 | National League · England |
| 2009-08-29 | Leyton OrientvCarlisleLeague One · England | 2–2 | League One · England |
| 2009-08-29 | Queen of SthvAyrChampionship · Scotland | 2–0 | Championship · Scotland |
| 2009-08-29 | BradfordvTorquayLeague Two · England | 2–0 | League Two · England |
| 2009-08-29 | KortrijkvGentPro League · Belgium | 1–0 | Pro League · Belgium |
| 2009-08-29 | MacclesfieldvRotherhamLeague Two · England | 1–3 | League Two · England |
| 2009-08-29 | ModenavAscoliSerie B · Italy | 1–2 | Serie B · Italy |
| 2009-08-29 | GironavCartagenaLa Liga 2 · Spain | 0–1 | La Liga 2 · Spain |
| 2009-08-29 | StockportvSouthamptonLeague One · England | 1–1 | League One · England |
| 2009-08-29 | LyonvNancyLigue 1 · France | 3–1 | Ligue 1 · France |
| 2009-08-29 | TottenhamvBirminghamPremier League · England | 2–1 | Premier League · England |
| 2009-08-29 | HannovervHoffenheimBundesliga · Germany | 0–1 | Bundesliga · Germany |
| 2009-08-29 | BarrowvTamworthNational League · England | 1–0 | National League · England |
| 2009-08-29 | NavalvPortoPrimeira Liga · Portugal | 1–3 | Primeira Liga · Portugal |
| 2009-08-29 | CrotonevBresciaSerie B · Italy | 0–0 | Serie B · Italy |
| 2009-08-29 | RangersvHamiltonPremiership · Scotland | 4–1 | Premiership · Scotland |
| 2009-08-29 | MilanvInterSerie A · Italy | 0–4 | Serie A · Italy |
| 2009-08-29 | KavalavAsteras TripolisSuper League · Greece | 0–1 | Super League · Greece |
| 2009-08-29 | AnconavSalernitanaSerie B · Italy | 2–0 | Serie B · Italy |
| 2009-08-29 | ChesterfieldvMorecambeLeague Two · England | 1–1 | League Two · England |
| 2009-08-29 | WycombevBristol RvsLeague One · England | 2–1 | League One · England |
| 2009-08-29 | PlymouthvSheffield WedsChampionship · England | 1–3 | Championship · England |
| 2009-08-29 | BrentfordvOldhamLeague One · England | 1–1 | League One · England |
| 2009-08-29 | StokevSunderlandPremier League · England | 1–0 | Premier League · England |
| 2009-08-29 | HerefordvPort ValeLeague Two · England | 2–2 | League Two · England |
| 2009-08-29 | BetisvCordobaLa Liga 2 · Spain | 3–0 | La Liga 2 · Spain |
| 2009-08-29 | WolvesvHullPremier League · England | 1–1 | Premier League · England |
| 2009-08-29 | NicevMontpellierLigue 1 · France | 0–3 | Ligue 1 · France |
| 2009-08-29 | ScunthorpevQPRChampionship · England | 0–1 | Championship · England |
| 2009-08-29 | AZ AlkmaarvWillem IIEredivisie · Netherlands | 2–1 | Eredivisie · Netherlands |
| 2009-08-29 | EskisehirsporvAntalyasporSüper Lig · Turkey | 2–1 | Süper Lig · Turkey |
| 2009-08-29 | YorkvHistonNational League · England | 3–1 | National League · England |
| 2009-08-29 | StirlingvPeterheadLeague One · Scotland | 2–1 | League One · Scotland |
| 2009-08-29 | ChelseavBurnleyPremier League · England | 3–0 | Premier League · England |
| 2009-08-29 | HuddersfieldvYeovilLeague One · England | 2–1 | League One · England |
| 2009-08-29 | AccringtonvShrewsburyLeague Two · England | 1–3 | League Two · England |
| 2009-08-29 | ForfarvEast StirlingLeague Two · Scotland | 5–1 | League Two · Scotland |
| 2009-08-29 | BurtonvNorthamptonLeague Two · England | 3–2 | League Two · England |
| 2009-08-29 | ElginvAnnan AthleticLeague Two · Scotland | 1–1 | League Two · Scotland |
| 2009-08-29 | Pacos FerreiravGuimaraesPrimeira Liga · Portugal | 0–0 | Primeira Liga · Portugal |
| 2009-08-29 | KidderminstervAltrinchamNational League · England | 3–0 | National League · England |
| 2009-08-29 | IpswichvPrestonChampionship · England | 1–1 | Championship · England |
| 2009-08-29 | RegginavPadovaSerie B · Italy | 1–1 | Serie B · Italy |
| 2009-08-29 | East FifevCowdenbeathLeague One · Scotland | 1–1 | League One · Scotland |
| 2009-08-29 | Kettering TownvLutonNational League · England | 0–0 | National League · England |
| 2009-08-29 | BarivBolognaSerie A · Italy | 0–0 | Serie A · Italy |
| 2009-08-29 | Sheffield UnitedvWest BromChampionship · England | 2–2 | Championship · England |
| 2009-08-29 | RoeselarevCharleroiPro League · Belgium | 1–3 | Pro League · Belgium |
| 2009-08-29 | AngersvAjaccioLigue 2 · France | 2–1 | Ligue 2 · France |
| 2009-08-29 | CambridgevGatesheadNational League · England | 3–0 | National League · England |