| 2009-09-12 | DortmundvBayern MunichBundesliga · Germany | 1–5 | Bundesliga · Germany |
| 2009-09-12 | Ath MadridvSantanderLa Liga · Spain | 1–1 | La Liga · Spain |
| 2009-09-12 | Forest GreenvEbbsfleetNational League · England | 0–0 | National League · England |
| 2009-09-12 | GillinghamvMillwallLeague One · England | 2–0 | League One · England |
| 2009-09-12 | PSV EindhovenvRoda JCEredivisie · Netherlands | 3–0 | Eredivisie · Netherlands |
| 2009-09-12 | Bristol RvsvOldhamLeague One · England | 1–0 | League One · England |
| 2009-09-12 | LutonvBarrowNational League · England | 1–0 | National League · England |
| 2009-09-12 | SevillavZaragozaLa Liga · Spain | 4–1 | La Liga · Spain |
| 2009-09-12 | BlackburnvWolvesPremier League · England | 3–1 | Premier League · England |
| 2009-09-12 | AlloavStirlingLeague One · Scotland | 1–0 | League One · Scotland |
| 2009-09-12 | Las PalmasvNumanciaLa Liga 2 · Spain | 1–0 | La Liga 2 · Spain |
| 2009-09-12 | West BromvPlymouthChampionship · England | 3–1 | Championship · England |
| 2009-09-12 | CrotonevCesenaSerie B · Italy | 0–0 | Serie B · Italy |
| 2009-09-12 | Munich 1860vGreuther Furth2. Bundesliga · Germany | 3–1 | 2. Bundesliga · Germany |
| 2009-09-12 | GrimsbyvHerefordLeague Two · England | 1–0 | League Two · England |
| 2009-09-12 | Dag and RedvChesterfieldLeague Two · England | 2–1 | League Two · England |
| 2009-09-12 | LaziovJuventusSerie A · Italy | 0–2 | Serie A · Italy |
| 2009-09-12 | MantovavRegginaSerie B · Italy | 2–2 | Serie B · Italy |
| 2009-09-12 | PortsmouthvBoltonPremier League · England | 2–3 | Premier League · England |
| 2009-09-12 | VicenzavAscoliSerie B · Italy | 2–2 | Serie B · Italy |
| 2009-09-12 | ValenciennesvBoulogneLigue 1 · France | 1–1 | Ligue 1 · France |
| 2009-09-12 | LillevSochauxLigue 1 · France | 1–0 | Ligue 1 · France |
| 2009-09-12 | Real UnionvCartagenaLa Liga 2 · Spain | 1–1 | La Liga 2 · Spain |
| 2009-09-12 | Den HaagvAZ AlkmaarEredivisie · Netherlands | 2–1 | Eredivisie · Netherlands |
| 2009-09-12 | GentvRoeselarePro League · Belgium | 5–1 | Pro League · Belgium |
| 2009-09-12 | MainzvHerthaBundesliga · Germany | 2–1 | Bundesliga · Germany |
| 2009-09-12 | ArbroathvBrechinLeague One · Scotland | 1–4 | League One · Scotland |
| 2009-09-12 | MortonvInverness CChampionship · Scotland | 0–3 | Championship · Scotland |
| 2009-09-12 | MiddlesbroughvIpswichChampionship · England | 3–1 | Championship · England |
| 2009-09-12 | Villarreal BvHerculesLa Liga 2 · Spain | 1–0 | La Liga 2 · Spain |
| 2009-09-12 | TranmerevWalsallLeague One · England | 2–3 | League One · England |
| 2009-09-12 | St TruidenvAnderlechtPro League · Belgium | 2–1 | Pro League · Belgium |
| 2009-09-12 | DumbartonvStenhousemuirLeague One · Scotland | 0–0 | League One · Scotland |
| 2009-09-12 | FrosinonevPadovaSerie B · Italy | 2–2 | Serie B · Italy |
| 2009-09-12 | LivornovMilanSerie A · Italy | 0–0 | Serie A · Italy |
| 2009-09-12 | RochdalevTorquayLeague Two · England | 2–1 | League Two · England |
| 2009-09-12 | ManisasporvSivassporSüper Lig · Turkey | 3–1 | Süper Lig · Turkey |
| 2009-09-12 | GimnasticvSociedadLa Liga 2 · Spain | 1–2 | La Liga 2 · Spain |
| 2009-09-12 | GatesheadvSalisburyNational League · England | 2–1 | National League · England |
| 2009-09-12 | MansfieldvStevenageNational League · England | 2–3 | National League · England |
| 2009-09-12 | HistonvKidderminsterNational League · England | 1–1 | National League · England |
| 2009-09-12 | WaregemvKortrijkPro League · Belgium | 0–2 | Pro League · Belgium |
| 2009-09-12 | CeltavLevanteLa Liga 2 · Spain | 1–1 | La Liga 2 · Spain |
| 2009-09-12 | BuryvCheltenhamLeague Two · England | 0–1 | League Two · England |
| 2009-09-12 | Rushden & DvGraysNational League · England | 5–4 | National League · England |
| 2009-09-12 | Hansa RostockvKoblenz2. Bundesliga · Germany | 2–1 | 2. Bundesliga · Germany |
| 2009-09-12 | AltrinchamvCrawley TownNational League · England | 0–0 | National League · England |
| 2009-09-12 | ArisvGianninaSuper League · Greece | 1–0 | Super League · Greece |
| 2009-09-12 | Notts CountyvNorthamptonLeague Two · England | 5–2 | League Two · England |
| 2009-09-12 | MontpelliervLensLigue 1 · France | 1–0 | Ligue 1 · France |