| 2009-09-26 | CeltavHerculesLa Liga 2 · Spain | 0–1 | La Liga 2 · Spain |
| 2009-09-26 | PadovavGallipoliSerie B · Italy | 0–0 | Serie B · Italy |
| 2009-09-26 | AntalyasporvFenerbahceSüper Lig · Turkey | 1–2 | Süper Lig · Turkey |
| 2009-09-26 | BuyuksehyrvSivassporSüper Lig · Turkey | 1–0 | Süper Lig · Turkey |
| 2009-09-26 | CarlislevSouthamptonLeague One · England | 1–1 | League One · England |
| 2009-09-26 | RotherhamvBarnetLeague Two · England | 3–0 | League Two · England |
| 2009-09-26 | SouthendvOldhamLeague One · England | 0–1 | League One · England |
| 2009-09-26 | Le HavrevMetzLigue 2 · France | 2–0 | Ligue 2 · France |
| 2009-09-26 | LokerenvCharleroiPro League · Belgium | 4–1 | Pro League · Belgium |
| 2009-09-26 | MontpelliervBoulogneLigue 1 · France | 1–0 | Ligue 1 · France |
| 2009-09-26 | TranmerevColchesterLeague One · England | 1–1 | League One · England |
| 2009-09-26 | PeterheadvDumbartonLeague One · Scotland | 1–2 | League One · Scotland |
| 2009-09-26 | KortrijkvStandardPro League · Belgium | 0–2 | Pro League · Belgium |
| 2009-09-26 | GaziantepsporvAnkaragucuSüper Lig · Turkey | 1–3 | Süper Lig · Turkey |
| 2009-09-26 | WaalwijkvRoda JCEredivisie · Netherlands | 4–1 | Eredivisie · Netherlands |
| 2009-09-26 | CambridgevLutonNational League · England | 3–4 | National League · England |
| 2009-09-26 | DerbyvBristol CityChampionship · England | 1–0 | Championship · England |
| 2009-09-26 | BirminghamvBoltonPremier League · England | 1–2 | Premier League · England |
| 2009-09-26 | NantesvNimesLigue 2 · France | 2–1 | Ligue 2 · France |
| 2009-09-26 | Dag and RedvMorecambeLeague Two · England | 1–1 | League Two · England |
| 2009-09-26 | ScunthorpevDoncasterChampionship · England | 2–2 | Championship · England |
| 2009-09-26 | CadizvRecreativoLa Liga 2 · Spain | 1–0 | La Liga 2 · Spain |
| 2009-09-26 | BrechinvStenhousemuirLeague One · Scotland | 1–0 | League One · Scotland |
| 2009-09-26 | Sheffield WedsvCardiffChampionship · England | 3–1 | Championship · England |
| 2009-09-26 | TottenhamvBurnleyPremier League · England | 5–0 | Premier League · England |
| 2009-09-26 | Eastbourne BoroughvTamworthNational League · England | 1–1 | National League · England |
| 2009-09-26 | St MirrenvCelticPremiership · Scotland | 0–2 | Premiership · Scotland |
| 2009-09-26 | LorientvParis SGLigue 1 · France | 1–1 | Ligue 1 · France |
| 2009-09-26 | Annan AthleticvAlbion RvsLeague Two · Scotland | 0–0 | League Two · Scotland |
| 2009-09-26 | GironavReal UnionLa Liga 2 · Spain | 0–2 | La Liga 2 · Spain |
| 2009-09-26 | GrossetovBresciaSerie B · Italy | 2–1 | Serie B · Italy |
| 2009-09-26 | GatesheadvOxfordNational League · England | 0–1 | National League · England |
| 2009-09-26 | AFC WimbledonvHistonNational League · England | 4–0 | National League · England |
| 2009-09-26 | GenkvRoeselarePro League · Belgium | 1–1 | Pro League · Belgium |
| 2009-09-26 | MurciavSalamancaLa Liga 2 · Spain | 2–2 | La Liga 2 · Spain |
| 2009-09-26 | RangersvAberdeenPremiership · Scotland | 0–0 | Premiership · Scotland |
| 2009-09-26 | PortovSp LisbonPrimeira Liga · Portugal | 1–0 | Primeira Liga · Portugal |
| 2009-09-26 | FrosinonevTorinoSerie B · Italy | 2–2 | Serie B · Italy |
| 2009-09-26 | MaritimovNavalPrimeira Liga · Portugal | 1–2 | Primeira Liga · Portugal |
| 2009-09-26 | West BromvCrystal PalaceChampionship · England | 0–1 | Championship · England |
| 2009-09-26 | Milton Keynes DonsvLeedsLeague One · England | 0–1 | League One · England |
| 2009-09-26 | DundeevRoss CountyChampionship · Scotland | 2–0 | Championship · Scotland |
| 2009-09-26 | MansfieldvHayes & YeadingNational League · England | 3–1 | National League · England |
| 2009-09-26 | BielefeldvSt Pauli2. Bundesliga · Germany | 1–0 | 2. Bundesliga · Germany |
| 2009-09-26 | Forest GreenvGraysNational League · England | 2–1 | National League · England |
| 2009-09-26 | SassuolovCesenaSerie B · Italy | 1–1 | Serie B · Italy |
| 2009-09-26 | East FifevClydeLeague One · Scotland | 1–0 | League One · Scotland |
| 2009-09-26 | StokevMan UnitedPremier League · England | 0–2 | Premier League · England |
| 2009-09-26 | AyrvRaith RvsChampionship · Scotland | 1–0 | Championship · Scotland |
| 2009-09-26 | Villarreal BvAlbaceteLa Liga 2 · Spain | 2–2 | La Liga 2 · Spain |