| 2009-10-17 | Albion RvsvQueens ParkLeague Two · Scotland | 0–1 | League Two · Scotland |
| 2009-10-17 | ModenavFrosinoneSerie B · Italy | 0–3 | Serie B · Italy |
| 2009-10-17 | Man UnitedvBoltonPremier League · England | 2–1 | Premier League · England |
| 2009-10-17 | PSV EindhovenvHeerenveenEredivisie · Netherlands | 1–0 | Eredivisie · Netherlands |
| 2009-10-17 | NAC BredavVitesseEredivisie · Netherlands | 4–0 | Eredivisie · Netherlands |
| 2009-10-17 | Leyton OrientvBrentfordLeague One · England | 2–1 | League One · England |
| 2009-10-17 | LyonvSochauxLigue 1 · France | 0–2 | Ligue 1 · France |
| 2009-10-17 | GrimsbyvRochdaleLeague Two · England | 0–2 | League Two · England |
| 2009-10-17 | Hayes & YeadingvCambridgeNational League · England | 3–0 | National League · England |
| 2009-10-17 | ElginvEast StirlingLeague Two · Scotland | 1–2 | League Two · Scotland |
| 2009-10-17 | StockportvMillwallLeague One · England | 0–4 | League One · England |
| 2009-10-17 | GenoavInterSerie A · Italy | 0–5 | Serie A · Italy |
| 2009-10-17 | BrechinvEast FifeLeague One · Scotland | 3–2 | League One · Scotland |
| 2009-10-17 | OlympiakosvAsteras TripolisSuper League Greece · Greece | 3–0 | Super League Greece · Greece |
| 2009-10-17 | ForfarvMontroseLeague Two · Scotland | 2–2 | League Two · Scotland |
| 2009-10-17 | TriestinavGallipoliSerie B · Italy | 1–0 | Serie B · Italy |
| 2009-10-17 | WrexhamvGraysNational League · England | 2–1 | National League · England |
| 2009-10-17 | Airdrie UtdvRoss CountyChampionship · Scotland | 0–1 | Championship · Scotland |
| 2009-10-17 | CittadellavPiacenzaSerie B · Italy | 1–1 | Serie B · Italy |
| 2009-10-17 | AccringtonvBournemouthLeague Two · England | 0–1 | League Two · England |
| 2009-10-17 | Bristol CityvPeterboroChampionship · England | 1–1 | Championship · England |
| 2009-10-17 | LivingstonvBerwickLeague Two · Scotland | 1–1 | League Two · Scotland |
| 2009-10-17 | VallecanovCadizLaLiga 2 · Spain | 2–2 | LaLiga 2 · Spain |
| 2009-10-17 | DarlingtonvShrewsburyLeague Two · England | 2–1 | League Two · England |
| 2009-10-17 | IpswichvSwanseaChampionship · England | 1–1 | Championship · England |
| 2009-10-17 | HamburgvLeverkusenBundesliga · Germany | 0–0 | Bundesliga · Germany |
| 2009-10-17 | PartickvAyrChampionship · Scotland | 2–0 | Championship · Scotland |
| 2009-10-17 | Sheffield WedsvCoventryChampionship · England | 2–0 | Championship · England |
| 2009-10-17 | CharltonvHuddersfieldLeague One · England | 2–1 | League One · England |
| 2009-10-17 | CesenavBresciaSerie B · Italy | 2–0 | Serie B · Italy |
| 2009-10-17 | Annan AthleticvStranraerLeague Two · Scotland | 1–0 | League Two · Scotland |
| 2009-10-17 | RegginavAnconaSerie B · Italy | 0–3 | Serie B · Italy |
| 2009-10-17 | Inverness CvDunfermlineChampionship · Scotland | 1–1 | Championship · Scotland |
| 2009-10-17 | Rushden & DvTamworthNational League · England | 3–2 | National League · England |
| 2009-10-17 | AjaxvWillem IIEredivisie · Netherlands | 4–0 | Eredivisie · Netherlands |
| 2009-10-17 | MiddlesbroughvWatfordChampionship · England | 0–1 | Championship · England |
| 2009-10-17 | WalsallvExeterLeague One · England | 3–0 | League One · England |
| 2009-10-17 | TranmerevBrightonLeague One · England | 2–1 | League One · England |
| 2009-10-17 | LeicestervDerbyChampionship · England | 0–0 | Championship · England |
| 2009-10-17 | DoncastervBarnsleyChampionship · England | 0–1 | Championship · England |
| 2009-10-17 | CrotonevVicenzaSerie B · Italy | 1–2 | Serie B · Italy |
| 2009-10-17 | Ein FrankfurtvHannoverBundesliga · Germany | 2–1 | Bundesliga · Germany |
| 2009-10-17 | ErgotelisvArisSuper League Greece · Greece | 0–0 | Super League Greece · Greece |
| 2009-10-17 | West BromvReadingChampionship · England | 3–1 | Championship · England |
| 2009-10-17 | CastellonvBetisLaLiga 2 · Spain | 1–0 | LaLiga 2 · Spain |
| 2009-10-17 | MortonvDundeeChampionship · Scotland | 0–1 | Championship · Scotland |
| 2009-10-17 | WesterlovStandardPro League · Belgium | 2–0 | Pro League · Belgium |
| 2009-10-17 | DenizlisporvBursasporSüper Lig · Türkiye | 2–3 | Süper Lig · Türkiye |
| 2009-10-17 | Milton Keynes DonsvGillinghamLeague One · England | 2–0 | League One · England |
| 2009-10-17 | NancyvMarseilleLigue 1 · France | 0–3 | Ligue 1 · France |