| 2010-10-16 | AFC WimbledonvGatesheadNational League · England | 1–0 | National League · England |
| 2010-10-16 | Forest GreenvHistonNational League · England | 0–1 | National League · England |
| 2010-10-16 | FrosinonevModenaSerie B · Italy | 1–1 | Serie B · Italy |
| 2010-10-16 | Werder BremenvFreiburgBundesliga · Germany | 2–1 | Bundesliga · Germany |
| 2010-10-16 | VVV VenlovAZ AlkmaarEredivisie · Netherlands | 0–1 | Eredivisie · Netherlands |
| 2010-10-16 | BarnsleyvNott'm ForestChampionship · England | 3–1 | Championship · England |
| 2010-10-16 | Eastbourne BoroughvLutonNational League · England | 2–4 | National League · England |
| 2010-10-16 | MacclesfieldvOxfordLeague Two · England | 3–2 | League Two · England |
| 2010-10-16 | GerminalvLiersePro League · Belgium | 1–1 | Pro League · Belgium |
| 2010-10-16 | Crystal PalacevMillwallChampionship · England | 0–1 | Championship · England |
| 2010-10-16 | StirlingvDundeeChampionship · Scotland | 1–1 | Championship · Scotland |
| 2010-10-16 | PanathinaikosvPAOKSuper League · Greece | 1–0 | Super League · Greece |
| 2010-10-16 | Port ValevGillinghamLeague Two · England | 0–0 | League Two · England |
| 2010-10-16 | StockportvBarnetLeague Two · England | 2–1 | League Two · England |
| 2010-10-16 | East FifevBrechinLeague One · Scotland | 1–3 | League One · Scotland |
| 2010-10-16 | Caenv MonacoLigue 1 · France | 0–0 | Ligue 1 · France |
| 2010-10-16 | Dag and RedvWalsallLeague One · England | 1–1 | League One · England |
| 2010-10-16 | BradfordvCheltenhamLeague Two · England | 3–1 | League Two · England |
| 2010-10-16 | ToulousevParis SGLigue 1 · France | 0–2 | Ligue 1 · France |
| 2010-10-16 | LivornovTorinoSerie B · Italy | 2–1 | Serie B · Italy |
| 2010-10-16 | EskisehirsporvBuyuksehyrSüper Lig · Turkey | 1–0 | Süper Lig · Turkey |
| 2010-10-16 | Notts CountyvPlymouthLeague One · England | 2–0 | League One · England |
| 2010-10-16 | MainzvHamburgBundesliga · Germany | 0–1 | Bundesliga · Germany |
| 2010-10-16 | BoltonvStokePremier League · England | 2–1 | Premier League · England |
| 2010-10-16 | ArbroathvClydeLeague Two · Scotland | 3–2 | League Two · Scotland |
| 2010-10-16 | Man UnitedvWest BromPremier League · England | 2–2 | Premier League · England |
| 2010-10-16 | CharltonvBrightonLeague One · England | 0–4 | League One · England |
| 2010-10-16 | Airdrie UtdvAlloaLeague One · Scotland | 0–1 | League One · Scotland |
| 2010-10-16 | ShrewsburyvLincolnLeague Two · England | 2–0 | League Two · England |
| 2010-10-16 | Den HaagvExcelsiorEredivisie · Netherlands | 2–1 | Eredivisie · Netherlands |
| 2010-10-16 | QPRvNorwichChampionship · England | 0–0 | Championship · England |
| 2010-10-16 | WolvesvWest HamPremier League · England | 1–1 | Premier League · England |
| 2010-10-16 | AjaxvNAC BredaEredivisie · Netherlands | 3–0 | Eredivisie · Netherlands |
| 2010-10-16 | Inverness CvSt JohnstonePremiership · Scotland | 1–1 | Premiership · Scotland |
| 2010-10-16 | Bristol RvsvRochdaleLeague One · England | 2–1 | League One · England |
| 2010-10-16 | St PaulivNurnbergBundesliga · Germany | 3–2 | Bundesliga · Germany |
| 2010-10-16 | AberdeenvHeartsPremiership · Scotland | 0–1 | Premiership · Scotland |
| 2010-10-16 | ArsenalvBirminghamPremier League · England | 2–1 | Premier League · England |
| 2010-10-16 | PescaravGrossetoSerie B · Italy | 4–2 | Serie B · Italy |
| 2010-10-16 | KarlsruhevBochum2. Bundesliga · Germany | 0–2 | 2. Bundesliga · Germany |
| 2010-10-16 | BetisvGironaLa Liga 2 · Spain | 2–1 | La Liga 2 · Spain |
| 2010-10-16 | Leyton OrientvHartlepoolLeague One · England | 1–0 | League One · England |
| 2010-10-16 | CharleroivLokerenPro League · Belgium | 1–2 | Pro League · Belgium |
| 2010-10-16 | Frankfurt FSVvHertha2. Bundesliga · Germany | 0–1 | 2. Bundesliga · Germany |
| 2010-10-16 | UtrechtvGraafschapEredivisie · Netherlands | 2–2 | Eredivisie · Netherlands |
| 2010-10-16 | Hayes & YeadingvWrexhamNational League · England | 0–3 | National League · England |
| 2010-10-16 | MilanvChievoSerie A · Italy | 3–1 | Serie A · Italy |
| 2010-10-16 | Bayern MunichvHannoverBundesliga · Germany | 3–0 | Bundesliga · Germany |
| 2010-10-16 | AuxerrevBordeauxLigue 1 · France | 0–1 | Ligue 1 · France |
| 2010-10-16 | ElginvQueens ParkLeague Two · Scotland | 4–2 | League Two · Scotland |