| 2009-10-24 | MacclesfieldvDag and RedLeague Two · England | 2–2 | League Two · England |
| 2009-10-24 | OlympiakosvErgotelisSuper League · Greece | 2–1 | Super League · Greece |
| 2009-10-24 | Boulognev MonacoLigue 1 · France | 1–3 | Ligue 1 · France |
| 2009-10-24 | HannovervStuttgartBundesliga · Germany | 1–0 | Bundesliga · Germany |
| 2009-10-24 | CoventryvWest BromChampionship · England | 0–0 | Championship · England |
| 2009-10-24 | Villarreal BvCastellonLa Liga 2 · Spain | 3–1 | La Liga 2 · Spain |
| 2009-10-24 | DumbartonvArbroathLeague One · Scotland | 1–0 | League One · Scotland |
| 2009-10-24 | ChesterfieldvBurtonLeague Two · England | 5–2 | League Two · England |
| 2009-10-24 | Bayern MunichvEin FrankfurtBundesliga · Germany | 2–1 | Bundesliga · Germany |
| 2009-10-24 | NAC BredavWaalwijkEredivisie · Netherlands | 1–0 | Eredivisie · Netherlands |
| 2009-10-24 | HullvPortsmouthPremier League · England | 0–0 | Premier League · England |
| 2009-10-24 | NewcastlevDoncasterChampionship · England | 2–1 | Championship · England |
| 2009-10-24 | BursasporvBuyuksehyrSüper Lig · Turkey | 6–0 | Süper Lig · Turkey |
| 2009-10-24 | Asteras TripolisvAtromitosSuper League · Greece | 1–2 | Super League · Greece |
| 2009-10-24 | PeterheadvAlloaLeague One · Scotland | 0–0 | League One · Scotland |
| 2009-10-24 | StirlingvClydeLeague One · Scotland | 1–1 | League One · Scotland |
| 2009-10-24 | Frankfurt FSVvMunich 18602. Bundesliga · Germany | 3–2 | 2. Bundesliga · Germany |
| 2009-10-24 | HeartsvFalkirkPremiership · Scotland | 0–0 | Premiership · Scotland |
| 2009-10-24 | MillwallvLeedsLeague One · England | 2–1 | League One · England |
| 2009-10-24 | BresciavAlbinoleffeSerie B · Italy | 4–1 | Serie B · Italy |
| 2009-10-24 | MainzvFreiburgBundesliga · Germany | 3–0 | Bundesliga · Germany |
| 2009-10-24 | SevillavEspanolLa Liga · Spain | 0–0 | La Liga · Spain |
| 2009-10-24 | BarnsleyvBristol CityChampionship · England | 2–3 | Championship · England |
| 2009-10-24 | AberdeenvDundee UnitedPremiership · Scotland | 0–2 | Premiership · Scotland |
| 2009-10-24 | Ross CountyvPartickChampionship · Scotland | 2–2 | Championship · Scotland |
| 2009-10-24 | GrenoblevNancyLigue 1 · France | 1–2 | Ligue 1 · France |
| 2009-10-24 | HartlepoolvTranmereLeague One · England | 1–0 | League One · England |
| 2009-10-24 | PrestonvMiddlesbroughChampionship · England | 2–2 | Championship · England |
| 2009-10-24 | HuddersfieldvLeyton OrientLeague One · England | 4–0 | League One · England |
| 2009-10-24 | SouthamptonvMilton Keynes DonsLeague One · England | 3–1 | League One · England |
| 2009-10-24 | Las PalmasvAlbaceteLa Liga 2 · Spain | 4–0 | La Liga 2 · Spain |
| 2009-10-24 | DundeevRaith RvsChampionship · Scotland | 2–1 | Championship · Scotland |
| 2009-10-24 | SampdoriavBolognaSerie A · Italy | 4–1 | Serie A · Italy |
| 2009-10-24 | SociedadvCordobaLa Liga 2 · Spain | 1–0 | La Liga 2 · Spain |
| 2009-10-24 | NorwichvSwindonLeague One · England | 1–0 | League One · England |
| 2009-10-24 | CesenavGrossetoSerie B · Italy | 2–2 | Serie B · Italy |
| 2009-10-24 | VitessevHeraclesEredivisie · Netherlands | 0–2 | Eredivisie · Netherlands |
| 2009-10-24 | SwanseavBlackpoolChampionship · England | 0–0 | Championship · England |
| 2009-10-24 | GironavBetisLa Liga 2 · Spain | 0–2 | La Liga 2 · Spain |
| 2009-10-24 | WolvesvAston VillaPremier League · England | 1–1 | Premier League · England |
| 2009-10-24 | BradfordvHerefordLeague Two · England | 1–0 | League Two · England |
| 2009-10-24 | Rio AvevSp BragaPrimeira Liga · Portugal | 1–1 | Primeira Liga · Portugal |
| 2009-10-24 | IntervCataniaSerie A · Italy | 2–1 | Serie A · Italy |
| 2009-10-24 | Cercle BruggevKortrijkPro League · Belgium | 1–1 | Pro League · Belgium |
| 2009-10-24 | StandardvWaregemPro League · Belgium | 1–1 | Pro League · Belgium |
| 2009-10-24 | St EtiennevValenciennesLigue 1 · France | 0–2 | Ligue 1 · France |
| 2009-10-24 | AnconavFrosinoneSerie B · Italy | 3–1 | Serie B · Italy |
| 2009-10-24 | BordeauxvLe MansLigue 1 · France | 3–0 | Ligue 1 · France |
| 2009-10-24 | GimnasticvHerculesLa Liga 2 · Spain | 1–1 | La Liga 2 · Spain |
| 2009-10-24 | LincolnvTorquayLeague Two · England | 0–0 | League Two · England |