| 2021-01-16 | Erzgebirge AuevFortuna Dusseldorf2. Bundesliga · Germany | 0–3 | 2. Bundesliga · Germany |
| 2021-01-16 | St JohnstonevSt MirrenPremiership · Scotland | 1–0 | Premiership · Scotland |
| 2021-01-16 | AngersvParis SGLigue 1 · France | 0–1 | Ligue 1 · France |
| 2021-01-16 | ColchestervCambridgeLeague Two · England | 1–1 | League Two · England |
| 2021-01-16 | ZwollevFor SittardEredivisie · Netherlands | 0–2 | Eredivisie · Netherlands |
| 2021-01-16 | SampdoriavUdineseSerie A · Italy | 2–1 | Serie A · Italy |
| 2021-01-16 | SwindonvDoncasterLeague One · England | 1–2 | League One · England |
| 2021-01-16 | MouscronvGenkPro League · Belgium | 2–0 | Pro League · Belgium |
| 2021-01-16 | HibernianvKilmarnockPremiership · Scotland | 2–0 | Premiership · Scotland |
| 2021-01-16 | Guadalajara ChivasvTolucaLiga MX · Mexico | 1–1 | Liga MX · Mexico |
| 2021-01-16 | RochdalevWiganLeague One · England | 3–3 | League One · England |
| 2021-01-16 | Bristol CityvPrestonChampionship · England | 2–0 | Championship · England |
| 2021-01-16 | PordenonevVeneziaSerie B · Italy | 2–0 | Serie B · Italy |
| 2021-01-16 | StuttgartvM'gladbachBundesliga · Germany | 2–2 | Bundesliga · Germany |
| 2021-01-16 | GaziantepvKayserisporSüper Lig · Turkey | 2–1 | Süper Lig · Turkey |
| 2021-01-16 | FluminensevSport RecifeSerie A · Brazil | 1–0 | Serie A · Brazil |
| 2021-01-16 | ClermontvDunkerqueLigue 2 · France | 5–0 | Ligue 2 · France |
| 2021-01-16 | NecaxavAtl. San LuisLiga MX · Mexico | 1–0 | Liga MX · Mexico |
| 2021-01-16 | BoltonvCheltenhamLeague Two · England | 1–1 | League Two · England |
| 2021-01-16 | FC EmmenvVitesseEredivisie · Netherlands | 1–4 | Eredivisie · Netherlands |
| 2021-01-16 | AntalyasporvTrabzonsporSüper Lig · Turkey | 1–1 | Süper Lig · Turkey |
| 2021-01-16 | ToulousevGrenobleLigue 2 · France | 2–0 | Ligue 2 · France |
| 2021-01-16 | LeicestervSouthamptonPremier League · England | 2–0 | Premier League · England |
| 2021-01-16 | TondelavBoavistaPrimeira Liga · Portugal | 3–1 | Primeira Liga · Portugal |
| 2021-01-16 | PisavBresciaSerie B · Italy | 1–0 | Serie B · Italy |
| 2021-01-16 | Asteras TripolisvPanetolikosSuper League · Greece | 2–0 | Super League · Greece |
| 2021-01-16 | CharleroivMechelenPro League · Belgium | 0–1 | Pro League · Belgium |
| 2021-01-16 | FulhamvChelseaPremier League · England | 0–1 | Premier League · England |
| 2021-01-16 | Pacos FerreiravSp BragaPrimeira Liga · Portugal | 2–0 | Primeira Liga · Portugal |
| 2021-01-16 | UtrechtvHeraclesEredivisie · Netherlands | 2–0 | Eredivisie · Netherlands |
| 2021-01-16 | CardiffvNorwichChampionship · England | 1–2 | Championship · England |
| 2021-01-16 | West HamvBurnleyPremier League · England | 1–0 | Premier League · England |
| 2021-01-16 | BlackburnvStokeChampionship · England | 1–1 | Championship · England |
| 2021-01-16 | LeedsvBrightonPremier League · England | 0–1 | Premier League · England |
| 2021-01-16 | AmiensvLe HavreLigue 2 · France | 0–0 | Ligue 2 · France |
| 2021-01-16 | JuarezvClub TijuanaLiga MX · Mexico | 0–0 | Liga MX · Mexico |
| 2021-01-16 | RodezvPau FCLigue 2 · France | 1–0 | Ligue 2 · France |
| 2021-01-16 | CelticvLivingstonPremiership · Scotland | 0–0 | Premiership · Scotland |
| 2021-01-16 | WolfsburgvRB LeipzigBundesliga · Germany | 2–2 | Bundesliga · Germany |
| 2021-01-16 | MonzavCosenzaSerie B · Italy | 2–2 | Serie B · Italy |
| 2021-01-16 | GrimsbyvSouthendLeague Two · England | 0–0 | League Two · England |
| 2021-01-16 | BarrowvScunthorpeLeague Two · England | 1–0 | League Two · England |
| 2021-01-16 | Fleetwood TownvPortsmouthLeague One · England | 0–1 | League One · England |
| 2021-01-16 | AlloavHeartsChampionship · Scotland | 1–3 | Championship · Scotland |
| 2021-01-16 | Leyton OrientvMorecambeLeague Two · England | 2–0 | League Two · England |
| 2021-01-16 | BarnsleyvSwanseaChampionship · England | 0–2 | Championship · England |
| 2021-01-16 | MarseillevNimesLigue 1 · France | 1–2 | Ligue 1 · France |
| 2021-01-16 | PeterborovMilton Keynes DonsLeague One · England | 3–0 | League One · England |
| 2021-01-16 | Nott'm ForestvMillwallChampionship · England | 3–1 | Championship · England |
| 2021-01-16 | AZ AlkmaarvDen HaagEredivisie · Netherlands | 2–1 | Eredivisie · Netherlands |