| 2021-01-23 | CambridgevBradfordLeague Two · England | 0–0 | League Two · England |
| 2021-01-23 | ChateaurouxvValenciennesLigue 2 · France | 3–3 | Ligue 2 · France |
| 2021-01-23 | FiorentinavCrotoneSerie A · Italy | 2–1 | Serie A · Italy |
| 2021-01-23 | WeymouthvHartlepoolNational League · England | 1–0 | National League · England |
| 2021-01-23 | Leyton OrientvForest GreenLeague Two · England | 0–1 | League Two · England |
| 2021-01-23 | DunkerquevAjaccioLigue 2 · France | 3–1 | Ligue 2 · France |
| 2021-01-23 | TranmerevBoltonLeague Two · England | 2–1 | League Two · England |
| 2021-01-23 | MorecambevColchesterLeague Two · England | 3–0 | League Two · England |
| 2021-01-23 | PortsmouthvHullLeague One · England | 0–4 | League One · England |
| 2021-01-23 | ChesterfieldvWrexhamNational League · England | 2–1 | National League · England |
| 2021-01-23 | FreiburgvStuttgartBundesliga · Germany | 2–1 | Bundesliga · Germany |
| 2021-01-23 | PSV EindhovenvWaalwijkEredivisie · Netherlands | 2–0 | Eredivisie · Netherlands |
| 2021-01-23 | LensvNiceLigue 1 · France | 0–1 | Ligue 1 · France |
| 2021-01-23 | ApollonvArisSuper League Greece · Greece | 0–1 | Super League Greece · Greece |
| 2021-01-23 | UdinesevInterSerie A · Italy | 0–0 | Serie A · Italy |
| 2021-01-23 | Sao PaulovCoritibaBrasileirão · Brazil | 1–1 | Brasileirão · Brazil |
| 2021-01-23 | AlavesvReal MadridLaLiga · Spain | 1–4 | LaLiga · Spain |
| 2021-01-23 | NancyvNiortLigue 2 · France | 2–2 | Ligue 2 · France |
| 2021-01-23 | SociedadvBetisLaLiga · Spain | 2–2 | LaLiga · Spain |
| 2021-01-23 | LincolnvNorthamptonLeague One · England | 2–1 | League One · England |
| 2021-01-23 | BromleyvHalifaxNational League · England | 1–2 | National League · England |
| 2021-01-23 | HerthavWerder BremenBundesliga · Germany | 1–4 | Bundesliga · Germany |
| 2021-01-23 | WealdstonevAldershotNational League · England | 3–4 | National League · England |
| 2021-01-23 | GironavEspanolLaLiga 2 · Spain | 1–0 | LaLiga 2 · Spain |
| 2021-01-23 | PanathinaikosvOFI CreteSuper League Greece · Greece | 2–0 | Super League Greece · Greece |
| 2021-01-23 | DunfermlinevAyrChampionship · Scotland | 0–0 | Championship · Scotland |
| 2021-01-23 | RiedvAustria ViennaAustrian Bundesliga · Austria | 0–1 | Austrian Bundesliga · Austria |
| 2021-01-23 | ExetervStevenageLeague Two · England | 3–1 | League Two · England |
| 2021-01-23 | VitessevGroningenEredivisie · Netherlands | 1–0 | Eredivisie · Netherlands |
| 2021-01-23 | Sepsi Sf. GheorghevAstraLiga I · Romania | 4–1 | Liga I · Romania |
| 2021-01-23 | LeverkusenvWolfsburgBundesliga · Germany | 0–1 | Bundesliga · Germany |
| 2021-01-23 | Queen of SthvMortonChampionship · Scotland | 2–1 | Championship · Scotland |
| 2021-01-23 | Port ValevWalsallLeague Two · England | 1–3 | League Two · England |
| 2021-01-23 | St. PoltenvAdmiraAustrian Bundesliga · Austria | 2–2 | Austrian Bundesliga · Austria |
| 2021-01-23 | FrosinonevRegginaSerie B · Italy | 1–1 | Serie B · Italy |
| 2021-01-23 | Wolfsberger ACvHartbergAustrian Bundesliga · Austria | 0–0 | Austrian Bundesliga · Austria |
| 2021-01-23 | Le HavrevGuingampLigue 2 · France | 1–1 | Ligue 2 · France |
| 2021-01-23 | St TruidenvMouscronPro League · Belgium | 0–2 | Pro League · Belgium |
| 2021-01-23 | MilanvAtalantaSerie A · Italy | 0–3 | Serie A · Italy |
| 2021-01-23 | WokingvMaidenheadNational League · England | 0–0 | National League · England |
| 2021-01-23 | MainzvRB LeipzigBundesliga · Germany | 3–2 | Bundesliga · Germany |
| 2021-01-23 | AugsburgvUnion BerlinBundesliga · Germany | 2–1 | Bundesliga · Germany |
| 2021-01-23 | BuyuksehyrvRizesporSüper Lig · Türkiye | 1–1 | Süper Lig · Türkiye |
| 2021-01-23 | CrewevAFC WimbledonLeague One · England | 1–1 | League One · England |
| 2021-01-23 | ReggianavVicenzaSerie B · Italy | 2–1 | Serie B · Italy |
| 2021-01-23 | IpswichvPeterboroLeague One · England | 0–1 | League One · England |
| 2021-01-23 | KarlsruhevHeidenheim2. Bundesliga · Germany | 1–1 | 2. Bundesliga · Germany |
| 2021-01-23 | Den HaagvFC EmmenEredivisie · Netherlands | 0–0 | Eredivisie · Netherlands |
| 2021-01-23 | UTA AradvFC ArgesLiga I · Romania | 1–2 | Liga I · Romania |
| 2021-01-23 | Notts CountyvTorquayNational League · England | 0–0 | National League · England |