| 2021-02-27 | TolucavAtlasLiga MX · Mexico | 0–0 | Liga MX · Mexico |
| 2021-02-27 | GrenoblevNiortLigue 2 · France | 1–1 | Ligue 2 · France |
| 2021-02-27 | SpeziavParmaSerie A · Italy | 2–2 | Serie A · Italy |
| 2021-02-27 | Raith RvsvAlloaChampionship · Scotland | 3–1 | Championship · Scotland |
| 2021-02-27 | LASKvHartbergBundesliga · Austria | 1–2 | Bundesliga · Austria |
| 2021-02-27 | AdmiravTirolBundesliga · Austria | 1–1 | Bundesliga · Austria |
| 2021-02-27 | RochdalevBurtonLeague One · England | 0–2 | League One · England |
| 2021-02-27 | WolfsburgvHerthaBundesliga · Germany | 2–0 | Bundesliga · Germany |
| 2021-02-27 | Santa ClaravPacos FerreiraPrimeira Liga · Portugal | 3–0 | Primeira Liga · Portugal |
| 2021-02-27 | Bayern MunichvFC KolnBundesliga · Germany | 5–1 | Bundesliga · Germany |
| 2021-02-27 | VallecanovPonferradinaLa Liga 2 · Spain | 1–1 | La Liga 2 · Spain |
| 2021-02-27 | Kashima AntlersvShimizu S-PulseJ1 League · Japan | 1–3 | J1 League · Japan |
| 2021-02-27 | St. GallenvBaselSuper League · Switzerland | 3–1 | Super League · Switzerland |
| 2021-02-27 | RB LeipzigvM'gladbachBundesliga · Germany | 3–2 | Bundesliga · Germany |
| 2021-02-27 | Lokomotiv MoscowvCSKA MoscowPremier League · Russia | 2–0 | Premier League · Russia |
| 2021-02-27 | Fleetwood TownvAccringtonLeague One · England | 1–1 | League One · England |
| 2021-02-27 | Forest GreenvColchesterLeague Two · England | 3–0 | League Two · England |
| 2021-02-27 | MiddlesbroughvCardiffChampionship · England | 1–1 | Championship · England |
| 2021-02-27 | HannovervGreuther Furth2. Bundesliga · Germany | 2–2 | 2. Bundesliga · Germany |
| 2021-02-27 | NewcastlevWolvesPremier League · England | 1–1 | Premier League · England |
| 2021-02-27 | KortrijkvWaregemPro League · Belgium | 1–2 | Pro League · Belgium |
| 2021-02-27 | VitessevVVV VenloEredivisie · Netherlands | 4–1 | Eredivisie · Netherlands |
| 2021-02-27 | St MirrenvRoss CountyPremiership · Scotland | 1–0 | Premiership · Scotland |
| 2021-02-27 | SpalvRegginaSerie B · Italy | 1–4 | Serie B · Italy |
| 2021-02-27 | RotherhamvReadingChampionship · England | 0–1 | Championship · England |
| 2021-02-27 | Mazatlan FCvQueretaroLiga MX · Mexico | 3–0 | Liga MX · Mexico |
| 2021-02-27 | CarlislevOldhamLeague Two · England | 1–3 | League Two · England |
| 2021-02-27 | ServettevZurichSuper League · Switzerland | 3–1 | Super League · Switzerland |
| 2021-02-27 | Cercle BruggevWaasland-BeverenPro League · Belgium | 2–0 | Pro League · Belgium |
| 2021-02-27 | CastellonvLas PalmasLa Liga 2 · Spain | 4–0 | La Liga 2 · Spain |
| 2021-02-27 | BanfieldvColon Santa FECopa De La Liga Profesional · Argentina | 0–3 | Copa De La Liga Profesional · Argentina |
| 2021-02-27 | AntalyasporvBuyuksehyrSüper Lig · Turkey | 0–0 | Süper Lig · Turkey |
| 2021-02-27 | KaragumrukvAlanyasporSüper Lig · Turkey | 2–0 | Süper Lig · Turkey |
| 2021-02-27 | PlymouthvLincolnLeague One · England | 4–3 | League One · England |
| 2021-02-27 | Chindia TargovistevUniv. CraiovaSuperliga · Romania | 1–0 | Superliga · Romania |
| 2021-02-27 | HarrogatevGrimsbyLeague Two · England | 1–0 | League Two · England |
| 2021-02-27 | MansfieldvMorecambeLeague Two · England | 1–0 | League Two · England |
| 2021-02-27 | BarnsleyvMillwallChampionship · England | 2–1 | Championship · England |
| 2021-02-27 | BrentfordvStokeChampionship · England | 2–1 | Championship · England |
| 2021-02-27 | PrestonvHuddersfieldChampionship · England | 3–0 | Championship · England |
| 2021-02-27 | Hokkaido Consadole SapporovYokohama FCJ1 League · Japan | 5–1 | J1 League · Japan |
| 2021-02-27 | AuxerrevRodezLigue 2 · France | 0–1 | Ligue 2 · France |
| 2021-02-27 | HataysporvAnkaragucuSüper Lig · Turkey | 4–1 | Süper Lig · Turkey |
| 2021-02-27 | LeedsvAston VillaPremier League · England | 0–1 | Premier League · England |
| 2021-02-27 | StockportvChesterfieldNational League · England | 2–0 | National League · England |
| 2021-02-27 | LogronesvMallorcaLa Liga 2 · Spain | 0–1 | La Liga 2 · Spain |
| 2021-02-27 | GalatasarayvErzurum BBSüper Lig · Turkey | 2–0 | Süper Lig · Turkey |
| 2021-02-27 | MonzavCittadellaSerie B · Italy | 0–0 | Serie B · Italy |
| 2021-02-27 | Vissel KobevGamba OsakaJ1 League · Japan | 1–0 | J1 League · Japan |
| 2021-02-27 | AyrvArbroathChampionship · Scotland | 0–1 | Championship · Scotland |