| 2016-02-20 | Mouscron-PeruwelzvOud-Heverlee LeuvenPro League · Belgium | 3–1 | Pro League · Belgium |
| 2016-02-20 | AyrvForfarLeague One · Scotland | 2–1 | League One · Scotland |
| 2016-02-20 | Ross CountyvHamiltonPremiership · Scotland | 2–1 | Premiership · Scotland |
| 2016-02-20 | MontrosevElginLeague Two · Scotland | 3–1 | League Two · Scotland |
| 2016-02-20 | AlcorconvAth Bilbao BLa Liga 2 · Spain | 1–0 | La Liga 2 · Spain |
| 2016-02-20 | SpeziavVirtus LancianoSerie B · Italy | 2–0 | Serie B · Italy |
| 2016-02-20 | BradfordvRochdaleLeague One · England | 2–2 | League One · England |
| 2016-02-20 | BurnleyvRotherhamChampionship · England | 2–0 | Championship · England |
| 2016-02-20 | Young BoysvThunSuper League · Switzerland | 2–1 | Super League · Switzerland |
| 2016-02-20 | AscolivComoSerie B · Italy | 1–0 | Serie B · Italy |
| 2016-02-20 | CambridgevYorkLeague Two · England | 3–1 | League Two · England |
| 2016-02-20 | NorthamptonvWycombeLeague Two · England | 1–0 | League Two · England |
| 2016-02-20 | SalzburgvAltachBundesliga · Austria | 2–0 | Bundesliga · Austria |
| 2016-02-20 | Wolfsberger ACvGrodigBundesliga · Austria | 2–0 | Bundesliga · Austria |
| 2016-02-20 | Forest GreenvEastleighNational League · England | 2–1 | National League · England |
| 2016-02-20 | BursasporvFenerbahceSüper Lig · Turkey | 0–0 | Süper Lig · Turkey |
| 2016-02-20 | ArbroathvQueens ParkLeague Two · Scotland | 0–1 | League Two · Scotland |
| 2016-02-20 | PrestonvSheffield WedsChampionship · England | 1–0 | Championship · England |
| 2016-02-20 | ChesterfieldvCreweLeague One · England | 3–1 | League One · England |
| 2016-02-20 | LivornovCesenaSerie B · Italy | 1–1 | Serie B · Italy |
| 2016-02-20 | TranmerevCheltenhamNational League · England | 0–1 | National League · England |
| 2016-02-20 | KilmarnockvDundeePremiership · Scotland | 0–0 | Premiership · Scotland |
| 2016-02-20 | PodbeskidzievLech PoznanEkstraklasa · Poland | 4–1 | Ekstraklasa · Poland |
| 2016-02-20 | BromleyvWokingNational League · England | 2–1 | National League · England |
| 2016-02-20 | BetisvSp GijonLa Liga · Spain | 1–1 | La Liga · Spain |
| 2016-02-20 | CarlislevStevenageLeague Two · England | 1–0 | League Two · England |
| 2016-02-20 | HerthavWolfsburgBundesliga · Germany | 1–1 | Bundesliga · Germany |
| 2016-02-20 | IraklisvAEKSuper League · Greece | 1–1 | Super League · Greece |
| 2016-02-20 | Boreham WoodvTorquayNational League · England | 0–1 | National League · England |
| 2016-02-20 | SwindonvBlackpoolLeague One · England | 3–2 | League One · England |
| 2016-02-20 | LevadeiakosvKallonisSuper League · Greece | 1–0 | Super League · Greece |
| 2016-02-20 | BrechinvDunfermlineLeague One · Scotland | 1–2 | League One · Scotland |
| 2016-02-20 | MallorcavGimnasticLa Liga 2 · Spain | 2–2 | La Liga 2 · Spain |
| 2016-02-20 | St JohnstonevMotherwellPremiership · Scotland | 2–1 | Premiership · Scotland |
| 2016-02-20 | OldhamvGillinghamLeague One · England | 2–1 | League One · England |
| 2016-02-20 | LutonvHartlepoolLeague Two · England | 2–1 | League Two · England |
| 2016-02-20 | MansfieldvDag and RedLeague Two · England | 3–2 | League Two · England |
| 2016-02-20 | LuganovZurichSuper League · Switzerland | 0–0 | Super League · Switzerland |
| 2016-02-20 | Las PalmasvBarcelonaLa Liga · Spain | 1–2 | La Liga · Spain |
| 2016-02-20 | VicenzavAvellinoSerie B · Italy | 0–0 | Serie B · Italy |
| 2016-02-20 | AltrinchamvGuiseleyNational League · England | 1–1 | National League · England |
| 2016-02-20 | BrentfordvDerbyChampionship · England | 1–3 | Championship · England |
| 2016-02-20 | CelticvInverness CPremiership · Scotland | 3–0 | Premiership · Scotland |
| 2016-02-20 | NovaravTernanaSerie B · Italy | 1–2 | Serie B · Italy |
| 2016-02-20 | MortonvFalkirkChampionship · Scotland | 0–1 | Championship · Scotland |
| 2016-02-20 | MillwallvPeterboroLeague One · England | 3–0 | League One · England |
| 2016-02-20 | CeltavEibarLa Liga · Spain | 3–2 | La Liga · Spain |
| 2016-02-20 | Le HavrevCreteilLigue 2 · France | 1–0 | Ligue 2 · France |
| 2016-02-20 | HalifaxvGrimsbyNational League · England | 4–2 | National League · England |
| 2016-02-20 | LugovLeganesLa Liga 2 · Spain | 1–2 | La Liga 2 · Spain |