| 2021-04-20 | Derry CityvDundalkPremier Division · Ireland | 1–1 | Premier Division · Ireland |
| 2021-04-20 | NorthamptonvIpswichLeague One · England | 3–0 | League One · England |
| 2021-04-20 | GrimsbyvMorecambeLeague Two · England | 0–3 | League Two · England |
| 2021-04-20 | AltachvAdmiraBundesliga · Austria | 0–1 | Bundesliga · Austria |
| 2021-04-20 | ShrewsburyvWiganLeague One · England | 1–2 | League One · England |
| 2021-04-20 | Bristol RvsvMilton Keynes DonsLeague One · England | 0–2 | League One · England |
| 2021-04-20 | ChesterfieldvHalifaxNational League · England | 1–2 | National League · England |
| 2021-04-20 | Ein FrankfurtvAugsburgBundesliga · Germany | 2–0 | Bundesliga · Germany |
| 2021-04-20 | Sheffield WedsvBlackburnChampionship · England | 1–0 | Championship · England |
| 2021-04-20 | MortonvHeartsChampionship · Scotland | 0–0 | Championship · Scotland |
| 2021-04-20 | BrentfordvCardiffChampionship · England | 1–1 | Championship · England |
| 2021-04-20 | Chongqing Liangjiang AthleticvShandong TaishanSuper League · China | 0–2 | Super League · China |
| 2021-04-20 | Boreham WoodvBarnetNational League · England | 0–0 | National League · England |
| 2021-04-20 | DroghedavShamrock RoversPremier Division · Ireland | 0–1 | Premier Division · Ireland |
| 2021-04-20 | BradfordvTranmereLeague Two · England | 0–1 | League Two · England |
| 2021-04-20 | Bayern MunichvLeverkusenBundesliga · Germany | 2–0 | Bundesliga · Germany |
| 2021-04-20 | KayserisporvGenclerbirligiSüper Lig · Turkey | 2–2 | Süper Lig · Turkey |
| 2021-04-20 | BrechinvCowdenbeathLeague Two · Scotland | 0–0 | League Two · Scotland |
| 2021-04-20 | GuingampvChamblyLigue 2 · France | 1–0 | Ligue 2 · France |
| 2021-04-20 | Queens ParkvElginLeague Two · Scotland | 0–0 | League Two · Scotland |
| 2021-04-20 | ChelseavBrightonPremier League · England | 0–0 | Premier League · England |
| 2021-04-20 | HataysporvAntalyasporSüper Lig · Turkey | 3–2 | Süper Lig · Turkey |
| 2021-04-20 | BielefeldvSchalke 04Bundesliga · Germany | 1–0 | Bundesliga · Germany |
| 2021-04-20 | HarrogatevOldhamLeague Two · England | 0–3 | League Two · England |
| 2021-04-20 | NiortvTroyesLigue 2 · France | 0–3 | Ligue 2 · France |
| 2021-04-20 | HullvSunderlandLeague One · England | 2–2 | League One · England |
| 2021-04-20 | WalsallvSalfordLeague Two · England | 0–2 | League Two · England |
| 2021-04-20 | Inverness CvDundeeChampionship · Scotland | 1–1 | Championship · Scotland |
| 2021-04-20 | Paris FCvClermontLigue 2 · France | 0–1 | Ligue 2 · France |
| 2021-04-20 | RochdalevBlackpoolLeague One · England | 1–0 | League One · England |
| 2021-04-20 | Annan AthleticvStenhousemuirLeague Two · Scotland | 5–1 | League Two · Scotland |
| 2021-04-20 | WealdstonevMaidenheadNational League · England | 0–6 | National League · England |
| 2021-04-20 | FC KolnvRB LeipzigBundesliga · Germany | 2–1 | Bundesliga · Germany |
| 2021-04-20 | ChateaurouxvRodezLigue 2 · France | 1–1 | Ligue 2 · France |
| 2021-04-20 | AFC WimbledonvOxfordLeague One · England | 2–1 | League One · England |
| 2021-04-20 | BurtonvLincolnLeague One · England | 0–1 | League One · England |
| 2021-04-20 | AmiensvValenciennesLigue 2 · France | 3–1 | Ligue 2 · France |
| 2021-04-20 | BohemiansvSligo RoversPremier Division · Ireland | 1–3 | Premier Division · Ireland |
| 2021-04-20 | FC HermannstadtvAstraSuperliga · Romania | 1–3 | Superliga · Romania |
| 2021-04-20 | Fleetwood TownvCreweLeague One · England | 0–2 | League One · England |
| 2021-04-20 | StevenagevCheltenhamLeague Two · England | 0–1 | League Two · England |
| 2021-04-20 | ColchestervSouthendLeague Two · England | 2–0 | League Two · England |
| 2021-04-20 | PlymouthvCharltonLeague One · England | 0–6 | League One · England |
| 2021-04-20 | Albion RvsvStranraerLeague Two · Scotland | 0–2 | League Two · Scotland |
| 2021-04-20 | Erzgebirge AuevNurnberg2. Bundesliga · Germany | 0–1 | 2. Bundesliga · Germany |
| 2021-04-20 | MansfieldvScunthorpeLeague Two · England | 3–0 | League Two · England |
| 2021-04-20 | GrenoblevSochauxLigue 2 · France | 2–0 | Ligue 2 · France |
| 2021-04-20 | StirlingvEdinburgh CityLeague Two · Scotland | 0–1 | League Two · Scotland |
| 2021-04-20 | SwindonvPortsmouthLeague One · England | 3–1 | League One · England |
| 2021-04-20 | Viitorul ConstantavGaz Metan MediasSuperliga · Romania | 1–0 | Superliga · Romania |