| 2021-04-24 | MainzvBayern MunichBundesliga · Germany | 2–1 | Bundesliga · Germany |
| 2021-04-24 | PeterheadvClydeLeague One · Scotland | 3–0 | League One · Scotland |
| 2021-04-24 | HalifaxvBromleyNational League · England | 1–2 | National League · England |
| 2021-04-24 | PueblavUNAM PumasLiga MX · Mexico | 0–0 | Liga MX · Mexico |
| 2021-04-24 | TranmerevBarrowLeague Two · England | 1–0 | League Two · England |
| 2021-04-24 | OxfordvPlymouthLeague One · England | 3–1 | League One · England |
| 2021-04-24 | ValenciavAlavesLa Liga · Spain | 1–1 | La Liga · Spain |
| 2021-04-24 | CFR ClujvUniv. CraiovaSuperliga · Romania | 1–2 | Superliga · Romania |
| 2021-04-24 | AuxerrevDunkerqueLigue 2 · France | 2–1 | Ligue 2 · France |
| 2021-04-24 | BarnsleyvRotherhamChampionship · England | 1–0 | Championship · England |
| 2021-04-24 | AltrinchamvBarnetNational League · England | 2–3 | National League · England |
| 2021-04-24 | ValladolidvCadizLa Liga · Spain | 1–1 | La Liga · Spain |
| 2021-04-24 | Boreham WoodvStockportNational League · England | 0–3 | National League · England |
| 2021-04-24 | TroyesvGrenobleLigue 2 · France | 3–1 | Ligue 2 · France |
| 2021-04-24 | PatronatovSarmiento JuninCopa De La Liga Profesional · Argentina | 1–0 | Copa De La Liga Profesional · Argentina |
| 2021-04-24 | EspanolvLas PalmasLa Liga 2 · Spain | 4–0 | La Liga 2 · Spain |
| 2021-04-24 | ZenitvR. VolgogradPremier League · Russia | 6–0 | Premier League · Russia |
| 2021-04-24 | MortonvAlloaChampionship · Scotland | 1–1 | Championship · Scotland |
| 2021-04-24 | CoventryvPrestonChampionship · England | 0–1 | Championship · England |
| 2021-04-24 | FreiburgvHoffenheimBundesliga · Germany | 1–1 | Bundesliga · Germany |
| 2021-04-24 | ElchevLevanteLa Liga · Spain | 1–0 | La Liga · Spain |
| 2021-04-24 | WaterfordvLongfordPremier Division · Ireland | 1–0 | Premier Division · Ireland |
| 2021-04-24 | Kashima AntlersvVissel KobeJ1 League · Japan | 1–1 | J1 League · Japan |
| 2021-04-24 | MiddlesbroughvSheffield WedsChampionship · England | 3–1 | Championship · England |
| 2021-04-24 | Sheffield UnitedvBrightonPremier League · England | 1–0 | Premier League · England |
| 2021-04-24 | Sparta RotterdamvVVV VenloEredivisie · Netherlands | 2–0 | Eredivisie · Netherlands |
| 2021-04-24 | AntalyasporvGalatasaraySüper Lig · Turkey | 0–1 | Süper Lig · Turkey |
| 2021-04-24 | GuingampvParis FCLigue 2 · France | 0–0 | Ligue 2 · France |
| 2021-04-24 | Nashville SCvCF MontrealMLS · United States | 2–2 | MLS · United States |
| 2021-04-24 | WiganvBurtonLeague One · England | 1–1 | League One · England |
| 2021-04-24 | SunderlandvAccringtonLeague One · England | 3–3 | League One · England |
| 2021-04-24 | GianninavPanetolikosSuper League · Greece | 0–1 | Super League · Greece |
| 2021-04-24 | Kashiwa ReysolvTokushimaJ1 League · Japan | 5–1 | J1 League · Japan |
| 2021-04-24 | QPRvNorwichChampionship · England | 1–3 | Championship · England |
| 2021-04-24 | West HamvChelseaPremier League · England | 0–1 | Premier League · England |
| 2021-04-24 | EastleighvSuttonNational League · England | 1–0 | National League · England |
| 2021-04-24 | New York CityvFC CincinnatiMLS · United States | 5–0 | MLS · United States |
| 2021-04-24 | Kings LynnvDag and RedNational League · England | 0–3 | National League · England |
| 2021-04-24 | LincolnvHullLeague One · England | 1–2 | League One · England |
| 2021-04-24 | AyrvQueen of SthChampionship · Scotland | 0–0 | Championship · Scotland |
| 2021-04-24 | Sanfrecce HiroshimavAvispa FukuokaJ1 League · Japan | 1–2 | J1 League · Japan |
| 2021-04-24 | ChamblyvNancyLigue 2 · France | 1–1 | Ligue 2 · France |
| 2021-04-24 | Edinburgh CityvStirlingLeague Two · Scotland | 0–1 | League Two · Scotland |
| 2021-04-24 | OsnabruckvHolstein Kiel2. Bundesliga · Germany | 1–3 | 2. Bundesliga · Germany |
| 2021-04-24 | CambridgevStevenageLeague Two · England | 0–1 | League Two · England |
| 2021-04-24 | NurnbergvHeidenheim2. Bundesliga · Germany | 3–1 | 2. Bundesliga · Germany |
| 2021-04-24 | CardiffvWycombeChampionship · England | 2–1 | Championship · England |
| 2021-04-24 | KonyasporvHataysporSüper Lig · Turkey | 0–0 | Süper Lig · Turkey |
| 2021-04-24 | East FifevDumbartonLeague One · Scotland | 2–1 | League One · Scotland |
| 2021-04-24 | MalagavFuenlabradaLa Liga 2 · Spain | 0–1 | La Liga 2 · Spain |