| 2009-02-21 | AvellinovVicenzaSerie B · Italy | 1–0 | Serie B · Italy |
| 2009-02-21 | ArsenalvSunderlandPremier League · England | 0–0 | Premier League · England |
| 2009-02-21 | ElginvAlbion RvsLeague Two · Scotland | 1–0 | League Two · Scotland |
| 2009-02-21 | PanathinaikosvPanioniosSuper League Greece · Greece | 2–1 | Super League Greece · Greece |
| 2009-02-21 | BielefeldvBochumBundesliga · Germany | 1–1 | Bundesliga · Germany |
| 2009-02-21 | HeraclesvAZ AlkmaarEredivisie · Netherlands | 0–2 | Eredivisie · Netherlands |
| 2009-02-21 | StenhousemuirvBerwickLeague Two · Scotland | 1–2 | League Two · Scotland |
| 2009-02-21 | LeedsvCheltenhamLeague One · England | 2–0 | League One · England |
| 2009-02-21 | BarnsleyvCharltonChampionship · England | 0–0 | Championship · England |
| 2009-02-21 | Kettering TownvTorquayNational League · England | 2–1 | National League · England |
| 2009-02-21 | EmpolivCittadellaSerie B · Italy | 0–1 | Serie B · Italy |
| 2009-02-21 | Annan AthleticvForfarLeague Two · Scotland | 1–0 | League Two · Scotland |
| 2009-02-21 | M'gladbachvHannoverBundesliga · Germany | 3–2 | Bundesliga · Germany |
| 2009-02-21 | MantovavTriestinaSerie B · Italy | 0–1 | Serie B · Italy |
| 2009-02-21 | NorthamptonvWalsallLeague One · England | 0–2 | League One · England |
| 2009-02-21 | Le HavrevAuxerreLigue 1 · France | 1–2 | Ligue 1 · France |
| 2009-02-21 | AlbacetevCastellonLaLiga 2 · Spain | 2–0 | LaLiga 2 · Spain |
| 2009-02-21 | CrewevHuddersfieldLeague One · England | 3–1 | League One · England |
| 2009-02-21 | DumbartonvCowdenbeathLeague Two · Scotland | 1–1 | League Two · Scotland |
| 2009-02-21 | WeymouthvRushden & DNational League · England | 0–9 | National League · England |
| 2009-02-21 | BoltonvWest HamPremier League · England | 2–1 | Premier League · England |
| 2009-02-21 | ElchevZaragozaLaLiga 2 · Spain | 2–0 | LaLiga 2 · Spain |
| 2009-02-21 | HartlepoolvLeyton OrientLeague One · England | 0–1 | League One · England |
| 2009-02-21 | HeerenveenvWillem IIEredivisie · Netherlands | 3–1 | Eredivisie · Netherlands |
| 2009-02-21 | PlymouthvSheffield UnitedChampionship · England | 2–2 | Championship · England |
| 2009-02-21 | Cercle BruggevLokerenPro League · Belgium | 0–0 | Pro League · Belgium |
| 2009-02-21 | BlackpoolvWatfordChampionship · England | 0–2 | Championship · England |
| 2009-02-21 | CarlislevStockportLeague One · England | 1–2 | League One · England |
| 2009-02-21 | Crawley TownvSalisburyNational League · England | 0–3 | National League · England |
| 2009-02-21 | DarlingtonvGrimsbyLeague Two · England | 1–0 | League Two · England |
| 2009-02-21 | HistonvEastbourne BoroughNational League · England | 3–3 | National League · England |
| 2009-02-21 | NancyvLyonLigue 1 · France | 0–2 | Ligue 1 · France |
| 2009-02-21 | BuryvNotts CountyLeague Two · England | 2–0 | League Two · England |
| 2009-02-21 | LutonvShrewsburyLeague Two · England | 3–1 | League Two · England |
| 2009-02-21 | MiddlesbroughvWiganPremier League · England | 0–0 | Premier League · England |
| 2009-02-21 | GentvGerminalPro League · Belgium | 1–3 | Pro League · Belgium |
| 2009-02-21 | StuttgartvHoffenheimBundesliga · Germany | 3–3 | Bundesliga · Germany |
| 2009-02-21 | TranmerevMilton Keynes DonsLeague One · England | 1–1 | League One · England |
| 2009-02-21 | YeovilvOldhamLeague One · England | 2–2 | League One · England |
| 2009-02-21 | Bayern MunichvFC KolnBundesliga · Germany | 1–2 | Bundesliga · Germany |
| 2009-02-21 | GenclerbirligivFenerbahceSüper Lig · Türkiye | 1–0 | Süper Lig · Türkiye |
| 2009-02-21 | GillinghamvMacclesfieldLeague Two · England | 3–1 | League Two · England |
| 2009-02-21 | MillwallvBrightonLeague One · England | 0–1 | League One · England |
| 2009-02-21 | RomavSienaSerie A · Italy | 1–0 | Serie A · Italy |
| 2009-02-21 | GrossetovLivornoSerie B · Italy | 2–3 | Serie B · Italy |
| 2009-02-21 | KonyasporvAntalyasporSüper Lig · Türkiye | 2–0 | Süper Lig · Türkiye |
| 2009-02-21 | KayserisporvBursasporSüper Lig · Türkiye | 0–0 | Süper Lig · Türkiye |
| 2009-02-21 | DendervMechelenPro League · Belgium | 1–2 | Pro League · Belgium |
| 2009-02-21 | VillarrealvSp GijonLaLiga · Spain | 2–1 | LaLiga · Spain |
| 2009-02-21 | PAOKvIraklisSuper League Greece · Greece | 1–0 | Super League Greece · Greece |