| 2010-02-13 | TamworthvMansfieldNational League · England | 2–4 | National League · England |
| 2010-02-13 | MorecambevDarlingtonLeague Two · England | 2–0 | League Two · England |
| 2010-02-13 | KilmarnockvSt JohnstonePremiership · Scotland | 3–2 | Premiership · Scotland |
| 2010-02-13 | BrentfordvWycombeLeague One · England | 1–1 | League One · England |
| 2010-02-13 | VallecanovGironaLa Liga 2 · Spain | 0–3 | La Liga 2 · Spain |
| 2010-02-13 | NumanciavLas PalmasLa Liga 2 · Spain | 3–1 | La Liga 2 · Spain |
| 2010-02-13 | MillwallvExeterLeague One · England | 1–0 | League One · England |
| 2010-02-13 | HannovervWerder BremenBundesliga · Germany | 1–5 | Bundesliga · Germany |
| 2010-02-13 | St MirrenvDundee UnitedPremiership · Scotland | 1–2 | Premiership · Scotland |
| 2010-02-13 | ShrewsburyvHerefordLeague Two · England | 3–1 | League Two · England |
| 2010-02-13 | CartagenavReal UnionLa Liga 2 · Spain | 1–0 | La Liga 2 · Spain |
| 2010-02-13 | Rushden & DvStevenageNational League · England | 1–0 | National League · England |
| 2010-02-13 | AberdeenvCelticPremiership · Scotland | 4–4 | Premiership · Scotland |
| 2010-02-13 | NancyvParis SGLigue 1 · France | 0–0 | Ligue 1 · France |
| 2010-02-13 | BrestvChateaurouxLigue 2 · France | 2–1 | Ligue 2 · France |
| 2010-02-13 | XerezvReal MadridLa Liga · Spain | 0–3 | La Liga · Spain |
| 2010-02-13 | StockportvColchesterLeague One · England | 2–2 | League One · England |
| 2010-02-13 | AlbinoleffevTorinoSerie B · Italy | 0–1 | Serie B · Italy |
| 2010-02-13 | Sheffield UnitedvBristol CityChampionship · England | 2–0 | Championship · England |
| 2010-02-13 | RegginavMantovaSerie B · Italy | 3–1 | Serie B · Italy |
| 2010-02-13 | ChesterfieldvBuryLeague Two · England | 1–0 | League Two · England |
| 2010-02-13 | GaziantepsporvBesiktasSüper Lig · Turkey | 2–0 | Süper Lig · Turkey |
| 2010-02-13 | CesenavCrotoneSerie B · Italy | 0–2 | Serie B · Italy |
| 2010-02-13 | LillevBoulogneLigue 1 · France | 3–1 | Ligue 1 · France |
| 2010-02-13 | LeicestervScunthorpeChampionship · England | 5–1 | Championship · England |
| 2010-02-13 | Hayes & YeadingvGatesheadNational League · England | 3–2 | National League · England |
| 2010-02-13 | Dag and RedvBournemouthLeague Two · England | 1–0 | League Two · England |
| 2010-02-13 | BarnsleyvPlymouthChampionship · England | 1–3 | Championship · England |
| 2010-02-13 | Crawley TownvAltrinchamNational League · England | 1–0 | National League · England |
| 2010-02-13 | LyonvLensLigue 1 · France | 1–0 | Ligue 1 · France |
| 2010-02-13 | AscolivVicenzaSerie B · Italy | 1–1 | Serie B · Italy |
| 2010-02-13 | RoeselarevKortrijkPro League · Belgium | 0–2 | Pro League · Belgium |
| 2010-02-13 | AlloavEast FifeLeague One · Scotland | 2–0 | League One · Scotland |
| 2010-02-13 | BerwickvForfarLeague Two · Scotland | 0–4 | League Two · Scotland |
| 2010-02-13 | KidderminstervForest GreenNational League · England | 2–1 | National League · England |
| 2010-02-13 | DiyarbakirsporvSivassporSüper Lig · Turkey | 1–1 | Süper Lig · Turkey |
| 2010-02-13 | BresciavPiacenzaSerie B · Italy | 1–0 | Serie B · Italy |
| 2010-02-13 | Raith RvsvInverness CChampionship · Scotland | 0–4 | Championship · Scotland |
| 2010-02-13 | Le MansvSochauxLigue 1 · France | 0–0 | Ligue 1 · France |
| 2010-02-13 | Den HaagvWillem IIEredivisie · Netherlands | 3–0 | Eredivisie · Netherlands |
| 2010-02-13 | PadovavFrosinoneSerie B · Italy | 0–0 | Serie B · Italy |
| 2010-02-13 | LeccevModenaSerie B · Italy | 0–0 | Serie B · Italy |
| 2010-02-13 | CarlislevMilton Keynes DonsLeague One · England | 5–0 | League One · England |
| 2010-02-13 | HerthavMainzBundesliga · Germany | 1–1 | Bundesliga · Germany |
| 2010-02-13 | LivingstonvAnnan AthleticLeague Two · Scotland | 3–2 | League Two · Scotland |
| 2010-02-13 | RecreativovBetisLa Liga 2 · Spain | 1–1 | La Liga 2 · Spain |
| 2010-02-13 | LincolnvRotherhamLeague Two · England | 1–2 | League Two · England |
| 2010-02-13 | MontpelliervGrenobleLigue 1 · France | 1–0 | Ligue 1 · France |
| 2010-02-13 | OxfordvHistonNational League · England | 2–0 | National League · England |
| 2010-02-13 | EbbsfleetvYorkNational League · England | 1–0 | National League · England |