| 2009-02-21 | Raith RvsvStirlingLeague One · Scotland | 1–1 | League One · Scotland |
| 2009-02-21 | VallecanovSevilla BLa Liga 2 · Spain | 1–0 | La Liga 2 · Spain |
| 2009-02-21 | SouthamptonvPrestonChampionship · England | 3–1 | Championship · England |
| 2009-02-21 | NantesvCaenLigue 1 · France | 1–1 | Ligue 1 · France |
| 2009-02-21 | Aston VillavChelseaPremier League · England | 0–1 | Premier League · England |
| 2009-02-21 | BarcelonavEspanolLa Liga · Spain | 1–2 | La Liga · Spain |
| 2009-02-21 | Bristol RvsvLeicesterLeague One · England | 0–1 | League One · England |
| 2009-02-21 | KarlsruhevEin FrankfurtBundesliga · Germany | 0–1 | Bundesliga · Germany |
| 2009-02-21 | SwindonvScunthorpeLeague One · England | 4–2 | League One · England |
| 2009-02-21 | ToulousevValenciennesLigue 1 · France | 0–0 | Ligue 1 · France |
| 2009-02-21 | HerefordvPeterboroLeague One · England | 0–1 | League One · England |
| 2009-02-21 | TrevisovModenaSerie B · Italy | 0–1 | Serie B · Italy |
| 2009-02-21 | OxfordvMansfieldNational League · England | 1–0 | National League · England |
| 2009-02-21 | PalermovJuventusSerie A · Italy | 0–2 | Serie A · Italy |
| 2009-02-21 | LewesvCambridgeNational League · England | 0–2 | National League · England |
| 2009-02-21 | ColchestervSouthendLeague One · England | 0–1 | League One · England |
| 2009-02-21 | BarivAscoliSerie B · Italy | 2–2 | Serie B · Italy |
| 2009-02-21 | RoeselarevBergenPro League · Belgium | 3–2 | Pro League · Belgium |
| 2009-02-21 | MontrosevEast StirlingLeague Two · Scotland | 0–2 | League Two · Scotland |
| 2009-02-21 | DunfermlinevRoss CountyChampionship · Scotland | 1–2 | Championship · Scotland |
| 2009-02-21 | NicevRennesLigue 1 · France | 0–1 | Ligue 1 · France |
| 2009-02-21 | BarrowvGraysNational League · England | 1–1 | National League · England |
| 2009-02-21 | DundeevLivingstonChampionship · Scotland | 4–1 | Championship · Scotland |
| 2009-02-21 | NorwichvBurnleyChampionship · England | 1–1 | Championship · England |
| 2009-02-21 | AyrvBrechinLeague One · Scotland | 4–2 | League One · Scotland |
| 2009-02-21 | XanthivOFISuper League · Greece | 0–0 | Super League · Greece |
| 2009-02-21 | Sp LisbonvBenficaPrimeira Liga · Portugal | 3–2 | Primeira Liga · Portugal |
| 2009-02-21 | AldershotvMorecambeLeague Two · England | 0–2 | League Two · England |
| 2009-02-21 | Real MadridvBetisLa Liga · Spain | 6–1 | La Liga · Spain |
| 2009-02-21 | WolfsburgvHerthaBundesliga · Germany | 2–1 | Bundesliga · Germany |
| 2009-02-21 | ReadingvBristol CityChampionship · England | 0–2 | Championship · England |
| 2009-02-21 | FalkirkvHamiltonPremiership · Scotland | 1–2 | Premiership · Scotland |
| 2009-02-21 | ChestervExeterLeague Two · England | 0–0 | League Two · England |
| 2009-02-21 | LorientvSochauxLigue 1 · France | 1–2 | Ligue 1 · France |
| 2009-02-21 | HeartsvSt MirrenPremiership · Scotland | 1–1 | Premiership · Scotland |
| 2009-02-21 | Las PalmasvMurciaLa Liga 2 · Spain | 0–1 | La Liga 2 · Spain |
| 2009-02-21 | BuyuksehyrvAnkaragucuSüper Lig · Turkey | 1–2 | Süper Lig · Turkey |
| 2009-02-21 | AccringtonvDag and RedLeague Two · England | 0–0 | League Two · England |
| 2009-02-20 | AjacciovBrestLigue 2 · France | 1–1 | Ligue 2 · France |
| 2009-02-20 | Schalke 04vDortmundBundesliga · Germany | 1–1 | Bundesliga · Germany |
| 2009-02-20 | TroyesvClermontLigue 2 · France | 0–1 | Ligue 2 · France |
| 2009-02-20 | ChesterfieldvBournemouthLeague Two · England | 1–0 | League Two · England |
| 2009-02-20 | StrasbourgvSedanLigue 2 · France | 1–3 | Ligue 2 · France |
| 2009-02-20 | GaziantepsporvBesiktasSüper Lig · Turkey | 0–3 | Süper Lig · Turkey |
| 2009-02-20 | BoulognevReimsLigue 2 · France | 0–3 | Ligue 2 · France |
| 2009-02-20 | VitessevRoda JCEredivisie · Netherlands | 3–0 | Eredivisie · Netherlands |
| 2009-02-20 | AmiensvBastiaLigue 2 · France | 0–1 | Ligue 2 · France |
| 2009-02-20 | GenkvMouscronPro League · Belgium | 1–1 | Pro League · Belgium |
| 2009-02-20 | Port ValevLincolnLeague Two · England | 0–1 | League Two · England |
| 2009-02-20 | GuingampvChateaurouxLigue 2 · France | 2–1 | Ligue 2 · France |