| 2009-02-21 | AlbacetevCastellonLa Liga 2 · Spain | 2–0 | La Liga 2 · Spain |
| 2009-02-21 | CrewevHuddersfieldLeague One · England | 3–1 | League One · England |
| 2009-02-21 | DumbartonvCowdenbeathLeague Two · Scotland | 1–1 | League Two · Scotland |
| 2009-02-21 | WeymouthvRushden & DNational League · England | 0–9 | National League · England |
| 2009-02-21 | BoltonvWest HamPremier League · England | 2–1 | Premier League · England |
| 2009-02-21 | ElchevZaragozaLa Liga 2 · Spain | 2–0 | La Liga 2 · Spain |
| 2009-02-21 | HartlepoolvLeyton OrientLeague One · England | 0–1 | League One · England |
| 2009-02-21 | HeerenveenvWillem IIEredivisie · Netherlands | 3–1 | Eredivisie · Netherlands |
| 2009-02-21 | PlymouthvSheffield UnitedChampionship · England | 2–2 | Championship · England |
| 2009-02-21 | Cercle BruggevLokerenPro League · Belgium | 0–0 | Pro League · Belgium |
| 2009-02-21 | BlackpoolvWatfordChampionship · England | 0–2 | Championship · England |
| 2009-02-21 | CarlislevStockportLeague One · England | 1–2 | League One · England |
| 2009-02-21 | Crawley TownvSalisburyNational League · England | 0–3 | National League · England |
| 2009-02-21 | DarlingtonvGrimsbyLeague Two · England | 1–0 | League Two · England |
| 2009-02-21 | HistonvEastbourne BoroughNational League · England | 3–3 | National League · England |
| 2009-02-21 | NancyvLyonLigue 1 · France | 0–2 | Ligue 1 · France |
| 2009-02-21 | BuryvNotts CountyLeague Two · England | 2–0 | League Two · England |
| 2009-02-21 | LutonvShrewsburyLeague Two · England | 3–1 | League Two · England |
| 2009-02-21 | MiddlesbroughvWiganPremier League · England | 0–0 | Premier League · England |
| 2009-02-21 | GentvGerminalPro League · Belgium | 1–3 | Pro League · Belgium |
| 2009-02-21 | StuttgartvHoffenheimBundesliga · Germany | 3–3 | Bundesliga · Germany |
| 2009-02-21 | TranmerevMilton Keynes DonsLeague One · England | 1–1 | League One · England |
| 2009-02-21 | YeovilvOldhamLeague One · England | 2–2 | League One · England |
| 2009-02-21 | Bayern MunichvFC KolnBundesliga · Germany | 1–2 | Bundesliga · Germany |
| 2009-02-21 | GenclerbirligivFenerbahceSüper Lig · Turkey | 1–0 | Süper Lig · Turkey |
| 2009-02-21 | GillinghamvMacclesfieldLeague Two · England | 3–1 | League Two · England |
| 2009-02-21 | MillwallvBrightonLeague One · England | 0–1 | League One · England |
| 2009-02-21 | RomavSienaSerie A · Italy | 1–0 | Serie A · Italy |
| 2009-02-21 | GrossetovLivornoSerie B · Italy | 2–3 | Serie B · Italy |
| 2009-02-21 | KonyasporvAntalyasporSüper Lig · Turkey | 2–0 | Süper Lig · Turkey |
| 2009-02-21 | KayserisporvBursasporSüper Lig · Turkey | 0–0 | Süper Lig · Turkey |
| 2009-02-21 | DendervMechelenPro League · Belgium | 1–2 | Pro League · Belgium |
| 2009-02-21 | VillarrealvSp GijonLa Liga · Spain | 2–1 | La Liga · Spain |
| 2009-02-21 | PAOKvIraklisSuper League · Greece | 1–0 | Super League · Greece |
| 2009-02-21 | GroningenvPSV EindhovenEredivisie · Netherlands | 0–1 | Eredivisie · Netherlands |
| 2009-02-21 | TrabzonsporvDenizlisporSüper Lig · Turkey | 0–2 | Süper Lig · Turkey |
| 2009-02-21 | Sheffield WedsvCrystal PalaceChampionship · England | 2–0 | Championship · England |
| 2009-02-21 | SwanseavDoncasterChampionship · England | 3–1 | Championship · England |
| 2009-02-21 | BolognavInterSerie A · Italy | 1–2 | Serie A · Italy |
| 2009-02-21 | KortrijkvCharleroiPro League · Belgium | 1–1 | Pro League · Belgium |
| 2009-02-21 | GrenoblevParis SGLigue 1 · France | 0–0 | Ligue 1 · France |
| 2009-02-21 | QPRvIpswichChampionship · England | 1–3 | Championship · England |
| 2009-02-21 | AlloavEast FifeLeague One · Scotland | 0–1 | League One · Scotland |
| 2009-02-21 | Nott'm ForestvDerbyChampionship · England | 1–3 | Championship · England |
| 2009-02-21 | CottbusvWerder BremenBundesliga · Germany | 2–1 | Bundesliga · Germany |
| 2009-02-21 | Rio AvevSetubalPrimeira Liga · Portugal | 1–0 | Primeira Liga · Portugal |
| 2009-02-21 | CeltavEibarLa Liga 2 · Spain | 1–2 | La Liga 2 · Spain |
| 2009-02-21 | Den HaagvNAC BredaEredivisie · Netherlands | 1–1 | Eredivisie · Netherlands |
| 2009-02-21 | RangersvKilmarnockPremiership · Scotland | 3–1 | Premiership · Scotland |
| 2009-02-21 | AltrinchamvWokingNational League · England | 1–0 | National League · England |