| 2009-02-28 | PeterborovSouthendLeague One · England | 1–2 | League One · England |
| 2009-02-28 | AscolivAvellinoSerie B · Italy | 2–1 | Serie B · Italy |
| 2009-02-28 | ChestervDag and RedLeague Two · England | 2–2 | League Two · England |
| 2009-02-28 | AuxerrevToulouseLigue 1 · France | 1–1 | Ligue 1 · France |
| 2009-02-28 | TranmerevSwindonLeague One · England | 1–0 | League One · England |
| 2009-02-28 | EvertonvWest BromPremier League · England | 2–0 | Premier League · England |
| 2009-02-28 | BrechinvEast FifeLeague One · Scotland | 2–1 | League One · Scotland |
| 2009-02-28 | PrestonvIpswichChampionship · England | 3–2 | Championship · England |
| 2009-02-28 | WalsallvYeovilLeague One · England | 2–0 | League One · England |
| 2009-02-28 | Inverness CvMotherwellPremiership · Scotland | 1–2 | Premiership · Scotland |
| 2009-02-28 | SwanseavCharltonChampionship · England | 1–1 | Championship · England |
| 2009-02-28 | LincolnvRotherhamLeague Two · England | 0–1 | League Two · England |
| 2009-02-28 | BurnleyvSheffield WedsChampionship · England | 2–4 | Championship · England |
| 2009-02-28 | SalernitanavPiacenzaSerie B · Italy | 0–1 | Serie B · Italy |
| 2009-02-28 | DortmundvHoffenheimBundesliga · Germany | 0–0 | Bundesliga · Germany |
| 2009-02-28 | VicenzavCittadellaSerie B · Italy | 1–1 | Serie B · Italy |
| 2009-02-28 | StranraervAlloaLeague One · Scotland | 1–3 | League One · Scotland |
| 2009-02-28 | Raith RvsvPeterheadLeague One · Scotland | 3–3 | League One · Scotland |
| 2009-02-28 | Ein FrankfurtvSchalke 04Bundesliga · Germany | 1–2 | Bundesliga · Germany |
| 2009-02-28 | CowdenbeathvStenhousemuirLeague Two · Scotland | 1–0 | League Two · Scotland |
| 2009-02-28 | ChesterfieldvBarnetLeague Two · England | 1–1 | League Two · England |
| 2009-02-28 | LarisavErgotelisSuper League · Greece | 3–2 | Super League · Greece |
| 2009-02-28 | FrosinonevBresciaSerie B · Italy | 0–3 | Serie B · Italy |
| 2009-02-28 | Pacos FerreiravRio AvePrimeira Liga · Portugal | 2–0 | Primeira Liga · Portugal |
| 2009-02-28 | Roda JCvGraafschapEredivisie · Netherlands | 3–1 | Eredivisie · Netherlands |
| 2009-02-28 | Bristol CityvBlackpoolChampionship · England | 0–0 | Championship · England |
| 2009-02-28 | AltrinchamvStevenageNational League · England | 1–2 | National League · England |
| 2009-02-28 | LivingstonvPartickChampionship · Scotland | 2–4 | Championship · Scotland |
| 2009-02-28 | WrexhamvSalisburyNational League · England | 1–1 | National League · England |
| 2009-02-28 | Ath BilbaovSevillaLa Liga · Spain | 1–2 | La Liga · Spain |
| 2009-02-28 | Notts CountyvBradfordLeague Two · England | 3–1 | League Two · England |
| 2009-02-28 | East StirlingvDumbartonLeague Two · Scotland | 3–1 | League Two · Scotland |
| 2009-02-28 | MurciavZaragozaLa Liga 2 · Spain | 1–4 | La Liga 2 · Spain |
| 2009-02-28 | CheltenhamvNorthamptonLeague One · England | 0–1 | League One · England |
| 2009-02-28 | RiminivAnconaSerie B · Italy | 2–1 | Serie B · Italy |
| 2009-02-28 | MiddlesbroughvLiverpoolPremier League · England | 2–0 | Premier League · England |
| 2009-02-28 | Port ValevLutonLeague Two · England | 1–3 | League Two · England |
| 2009-02-28 | AlavesvSociedadLa Liga 2 · Spain | 2–1 | La Liga 2 · Spain |
| 2009-02-28 | Le MansvLe HavreLigue 1 · France | 2–0 | Ligue 1 · France |
| 2009-02-28 | ArsenalvFulhamPremier League · England | 0–0 | Premier League · England |
| 2009-02-28 | AberdeenvKilmarnockPremiership · Scotland | 0–0 | Premiership · Scotland |
| 2009-02-28 | EspanolvReal MadridLa Liga · Spain | 0–2 | La Liga · Spain |
| 2009-02-28 | SouthamptonvCardiffChampionship · England | 1–0 | Championship · England |
| 2009-02-28 | XerezvVallecanoLa Liga 2 · Spain | 1–1 | La Liga 2 · Spain |
| 2009-02-28 | YorkvWeymouthNational League · England | 2–0 | National League · England |
| 2009-02-28 | JuventusvNapoliSerie A · Italy | 1–0 | Serie A · Italy |
| 2009-02-28 | LaziovBolognaSerie A · Italy | 2–0 | Serie A · Italy |
| 2009-02-28 | ElginvMontroseLeague Two · Scotland | 1–0 | League Two · Scotland |
| 2009-02-28 | ColchestervHartlepoolLeague One · England | 1–1 | League One · England |
| 2009-02-28 | AnkarasporvGenclerbirligiSüper Lig · Turkey | 0–0 | Süper Lig · Turkey |