| 2010-02-24 | RoeselarevGenkPro League · Belgium | 1–1 | Pro League · Belgium |
| 2010-02-23 | Milton Keynes DonsvMillwallLeague One · England | 1–3 | League One · England |
| 2010-02-23 | Port ValevLincolnLeague Two · England | 4–0 | League Two · England |
| 2010-02-23 | Man UnitedvWest HamPremier League · England | 3–0 | Premier League · England |
| 2010-02-23 | CheltenhamvTorquayLeague Two · England | 1–1 | League Two · England |
| 2010-02-23 | BuryvMorecambeLeague Two · England | 0–0 | League Two · England |
| 2010-02-23 | NorwichvSouthendLeague One · England | 2–1 | League One · England |
| 2010-02-23 | WalsallvYeovilLeague One · England | 0–1 | League One · England |
| 2010-02-23 | TranmerevHuddersfieldLeague One · England | 0–2 | League One · England |
| 2010-02-23 | GrimsbyvMacclesfieldLeague Two · England | 1–1 | League Two · England |
| 2010-02-23 | LeedsvOldhamLeague One · England | 2–0 | League One · England |
| 2010-02-23 | WycombevSouthamptonLeague One · England | 0–0 | League One · England |
| 2010-02-23 | HerefordvNorthamptonLeague Two · England | 0–2 | League Two · England |
| 2010-02-23 | TamworthvKettering TownNational League · England | 1–3 | National League · England |
| 2010-02-23 | HartlepoolvCarlisleLeague One · England | 4–1 | League One · England |
| 2010-02-23 | AldershotvDag and RedLeague Two · England | 2–3 | League Two · England |
| 2010-02-23 | GatesheadvAltrinchamNational League · England | 1–0 | National League · England |
| 2010-02-23 | DoncastervLeicesterChampionship · England | 0–1 | Championship · England |
| 2010-02-23 | CharltonvBrightonLeague One · England | 1–2 | League One · England |
| 2010-02-23 | GillinghamvLeyton OrientLeague One · England | 1–1 | League One · England |
| 2010-02-23 | RochdalevBradfordLeague Two · England | 1–3 | League Two · England |
| 2010-02-23 | ScunthorpevIpswichChampionship · England | 1–1 | Championship · England |
| 2010-02-23 | OxfordvAFC WimbledonNational League · England | 2–0 | National League · England |
| 2010-02-23 | SwindonvStockportLeague One · England | 4–1 | League One · England |
| 2010-02-23 | BournemouthvBarnetLeague Two · England | 3–0 | League Two · England |
| 2010-02-22 | ValenciavGetafeLa Liga · Spain | 2–1 | La Liga · Spain |
| 2010-02-22 | FenerbahcevBursasporSüper Lig · Turkey | 2–3 | Süper Lig · Turkey |
| 2010-02-22 | CrotonevLecceSerie B · Italy | 1–1 | Serie B · Italy |
| 2010-02-22 | KaiserslauternvSt Pauli2. Bundesliga · Germany | 3–0 | 2. Bundesliga · Germany |
| 2010-02-22 | BelenensesvAcademicaPrimeira Liga · Portugal | 1–2 | Primeira Liga · Portugal |
| 2010-02-22 | RotherhamvShrewsburyLeague Two · England | 1–1 | League Two · England |
| 2010-02-21 | AtalantavChievoSerie A · Italy | 0–1 | Serie A · Italy |
| 2010-02-21 | GironavSociedadLa Liga 2 · Spain | 1–0 | La Liga 2 · Spain |
| 2010-02-21 | AnderlechtvMechelenPro League · Belgium | 2–0 | Pro League · Belgium |
| 2010-02-21 | ZaragozavSp GijonLa Liga · Spain | 1–3 | La Liga · Spain |
| 2010-02-21 | Real UnionvVallecanoLa Liga 2 · Spain | 2–1 | La Liga 2 · Spain |
| 2010-02-21 | CottbusvPaderborn2. Bundesliga · Germany | 1–2 | 2. Bundesliga · Germany |
| 2010-02-21 | OberhausenvGreuther Furth2. Bundesliga · Germany | 0–1 | 2. Bundesliga · Germany |
| 2010-02-21 | Werder BremenvLeverkusenBundesliga · Germany | 2–2 | Bundesliga · Germany |
| 2010-02-21 | MurciavNumanciaLa Liga 2 · Spain | 1–0 | La Liga 2 · Spain |
| 2010-02-21 | Aston VillavBurnleyPremier League · England | 5–2 | Premier League · England |
| 2010-02-21 | Club BruggevStandardPro League · Belgium | 2–1 | Pro League · Belgium |
| 2010-02-21 | Rio AvevPacos FerreiraPrimeira Liga · Portugal | 1–2 | Primeira Liga · Portugal |
| 2010-02-21 | CagliarivParmaSerie A · Italy | 2–0 | Serie A · Italy |
| 2010-02-21 | DiyarbakirsporvDenizlisporSüper Lig · Turkey | 0–2 | Süper Lig · Turkey |
| 2010-02-21 | Las PalmasvSalamancaLa Liga 2 · Spain | 2–2 | La Liga 2 · Spain |
| 2010-02-21 | TwentevWillem IIEredivisie · Netherlands | 1–0 | Eredivisie · Netherlands |
| 2010-02-21 | SienavNapoliSerie A · Italy | 0–0 | Serie A · Italy |
| 2010-02-21 | MalagavEspanolLa Liga · Spain | 2–1 | La Liga · Spain |
| 2010-02-21 | FiorentinavLivornoSerie A · Italy | 2–1 | Serie A · Italy |