| 2010-02-28 | PanthrakikosvKavalaSuper League · Greece | 1–3 | Super League · Greece |
| 2010-02-28 | LivornovSienaSerie A · Italy | 1–2 | Serie A · Italy |
| 2010-02-28 | TottenhamvEvertonPremier League · England | 2–1 | Premier League · England |
| 2010-02-28 | ManisasporvDiyarbakirsporSüper Lig · Turkey | 2–1 | Süper Lig · Turkey |
| 2010-02-28 | ChievovCagliariSerie A · Italy | 2–1 | Serie A · Italy |
| 2010-02-28 | PanioniosvPanathinaikosSuper League · Greece | 0–2 | Super League · Greece |
| 2010-02-27 | Annan AthleticvEast StirlingLeague Two · Scotland | 1–0 | League Two · Scotland |
| 2010-02-27 | LyonvNiceLigue 1 · France | 2–0 | Ligue 1 · France |
| 2010-02-27 | MacclesfieldvRochdaleLeague Two · England | 0–1 | League Two · England |
| 2010-02-27 | SalernitanavPiacenzaSerie B · Italy | 1–0 | Serie B · Italy |
| 2010-02-27 | KayserisporvBesiktasSüper Lig · Turkey | 1–2 | Süper Lig · Turkey |
| 2010-02-27 | NumanciavGimnasticLa Liga 2 · Spain | 1–1 | La Liga 2 · Spain |
| 2010-02-27 | SalamancavMurciaLa Liga 2 · Spain | 0–2 | La Liga 2 · Spain |
| 2010-02-27 | MainzvWerder BremenBundesliga · Germany | 1–2 | Bundesliga · Germany |
| 2010-02-27 | LincolnvCreweLeague Two · England | 1–1 | League Two · England |
| 2010-02-27 | Sp BragavOlhanensePrimeira Liga · Portugal | 3–1 | Primeira Liga · Portugal |
| 2010-02-27 | AEKvErgotelisSuper League · Greece | 1–0 | Super League · Greece |
| 2010-02-27 | Rushden & DvKettering TownNational League · England | 0–0 | National League · England |
| 2010-02-27 | BarnsleyvBlackpoolChampionship · England | 1–0 | Championship · England |
| 2010-02-27 | WatfordvNewcastleChampionship · England | 1–2 | Championship · England |
| 2010-02-27 | VitessevAZ AlkmaarEredivisie · Netherlands | 0–3 | Eredivisie · Netherlands |
| 2010-02-27 | SouthamptonvWalsallLeague One · England | 5–1 | League One · England |
| 2010-02-27 | MiddlesbroughvQPRChampionship · England | 2–0 | Championship · England |
| 2010-02-27 | AlbinoleffevMantovaSerie B · Italy | 1–0 | Serie B · Italy |
| 2010-02-27 |  MonacovBoulogneLigue 1 · France | 1–0 | Ligue 1 · France |
| 2010-02-27 | BradfordvDarlingtonLeague Two · England | 1–0 | League Two · England |
| 2010-02-27 | LeverkusenvFC KolnBundesliga · Germany | 0–0 | Bundesliga · Germany |
| 2010-02-27 | TenerifevReal MadridLa Liga · Spain | 1–5 | La Liga · Spain |
| 2010-02-27 | NavalvMaritimoPrimeira Liga · Portugal | 2–1 | Primeira Liga · Portugal |
| 2010-02-27 | StockportvWycombeLeague One · England | 4–3 | League One · England |
| 2010-02-27 | RecreativovCadizLa Liga 2 · Spain | 2–1 | La Liga 2 · Spain |
| 2010-02-27 | HerthavHoffenheimBundesliga · Germany | 0–2 | Bundesliga · Germany |
| 2010-02-27 | EbbsfleetvBarrowNational League · England | 1–4 | National League · England |
| 2010-02-27 | HibernianvSt JohnstonePremiership · Scotland | 1–1 | Premiership · Scotland |
| 2010-02-27 | ReadingvSheffield WedsChampionship · England | 5–0 | Championship · England |
| 2010-02-27 | KoblenzvAugsburg2. Bundesliga · Germany | 0–1 | 2. Bundesliga · Germany |
| 2010-02-27 | DoncastervCrystal PalaceChampionship · England | 1–1 | Championship · England |
| 2010-02-27 | AlloavPeterheadLeague One · Scotland | 2–1 | League One · Scotland |
| 2010-02-27 | TorquayvAccringtonLeague Two · England | 2–1 | League Two · England |
| 2010-02-27 | AlbacetevVillarreal BLa Liga 2 · Spain | 1–1 | La Liga 2 · Spain |
| 2010-02-27 | Greuther FurthvHansa Rostock2. Bundesliga · Germany | 1–0 | 2. Bundesliga · Germany |
| 2010-02-27 | YeovilvMilton Keynes DonsLeague One · England | 1–0 | League One · England |
| 2010-02-27 | Cercle BruggevGenkPro League · Belgium | 1–0 | Pro League · Belgium |
| 2010-02-27 | LeccevTriestinaSerie B · Italy | 1–1 | Serie B · Italy |
| 2010-02-27 | West BromvDerbyChampionship · England | 3–1 | Championship · England |
| 2010-02-27 | St MirrenvHamiltonPremiership · Scotland | 0–0 | Premiership · Scotland |
| 2010-02-27 | Asteras TripolisvIraklisSuper League · Greece | 0–4 | Super League · Greece |
| 2010-02-27 | StuttgartvEin FrankfurtBundesliga · Germany | 2–1 | Bundesliga · Germany |
| 2010-02-27 | Sheffield UnitedvPlymouthChampionship · England | 4–3 | Championship · England |
| 2010-02-27 | LorientvSochauxLigue 1 · France | 1–0 | Ligue 1 · France |