| 2009-03-14 | LokerenvCharleroiPro League · Belgium | 0–0 | Pro League · Belgium |
| 2009-03-14 | BrightonvYeovilLeague One · England | 5–0 | League One · England |
| 2009-03-14 | Annan AthleticvCowdenbeathLeague Two · Scotland | 3–1 | League Two · Scotland |
| 2009-03-14 | WolvesvCharltonChampionship · England | 2–1 | Championship · England |
| 2009-03-14 | CagliarivGenoaSerie A · Italy | 0–1 | Serie A · Italy |
| 2009-03-14 | CambridgevNorthwichNational League · England | 4–1 | National League · England |
| 2009-03-14 | Sheffield UnitedvDerbyChampionship · England | 4–2 | Championship · England |
| 2009-03-14 | ValenciavRecreativoLa Liga · Spain | 1–1 | La Liga · Spain |
| 2009-03-14 | Ein FrankfurtvHoffenheimBundesliga · Germany | 1–1 | Bundesliga · Germany |
| 2009-03-14 | LincolnvBuryLeague Two · England | 1–1 | League Two · England |
| 2009-03-14 | Kettering TownvEastbourne BoroughNational League · England | 0–1 | National League · England |
| 2009-03-14 | JuventusvBolognaSerie A · Italy | 4–1 | Serie A · Italy |
| 2009-03-14 | AnkaragucuvSivassporSüper Lig · Turkey | 0–2 | Süper Lig · Turkey |
| 2009-03-14 | Willem IIvTwenteEredivisie · Netherlands | 0–2 | Eredivisie · Netherlands |
| 2009-03-14 | CastellonvVallecanoLa Liga 2 · Spain | 0–0 | La Liga 2 · Spain |
| 2009-03-14 | TubizevWesterloPro League · Belgium | 1–2 | Pro League · Belgium |
| 2009-03-14 | Inverness CvKilmarnockPremiership · Scotland | 2–1 | Premiership · Scotland |
| 2009-03-14 | Sevilla BvLas PalmasLa Liga 2 · Spain | 2–2 | La Liga 2 · Spain |
| 2009-03-14 | MacclesfieldvBournemouthLeague Two · England | 0–2 | League Two · England |
| 2009-03-14 | PeterheadvEast FifeLeague One · Scotland | 2–0 | League One · Scotland |
| 2009-03-14 | DoncastervBirminghamChampionship · England | 0–2 | Championship · England |
| 2009-03-14 | HerefordvSouthendLeague One · England | 0–1 | League One · England |
| 2009-03-14 | RochdalevRotherhamLeague Two · England | 1–2 | League Two · England |
| 2009-03-14 | CarlislevScunthorpeLeague One · England | 1–1 | League One · England |
| 2009-03-14 | AEKvOFISuper League · Greece | 1–0 | Super League · Greece |
| 2009-03-14 | HeraclesvVolendamEredivisie · Netherlands | 1–1 | Eredivisie · Netherlands |
| 2009-03-14 | MantovavSassuoloSerie B · Italy | 1–1 | Serie B · Italy |
| 2009-03-14 | Man UnitedvLiverpoolPremier League · England | 1–4 | Premier League · England |
| 2009-03-14 | Raith RvsvAyrLeague One · Scotland | 0–1 | League One · Scotland |
| 2009-03-14 | VitessevNAC BredaEredivisie · Netherlands | 0–3 | Eredivisie · Netherlands |
| 2009-03-14 | AntalyasporvBuyuksehyrSüper Lig · Turkey | 1–0 | Süper Lig · Turkey |
| 2009-03-14 | GillinghamvShrewsburyLeague Two · England | 2–2 | League Two · England |
| 2009-03-14 | DundeevRoss CountyChampionship · Scotland | 2–0 | Championship · Scotland |
| 2009-03-14 | GimnasticvCordobaLa Liga 2 · Spain | 2–2 | La Liga 2 · Spain |
| 2009-03-14 | ModenavAnconaSerie B · Italy | 1–0 | Serie B · Italy |
| 2009-03-14 | NantesvLorientLigue 1 · France | 1–1 | Ligue 1 · France |
| 2009-03-14 | MorecambevBarnetLeague Two · England | 2–1 | League Two · England |
| 2009-03-14 | StirlingvBrechinLeague One · Scotland | 2–3 | League One · Scotland |
| 2009-03-14 | BarnsleyvBlackpoolChampionship · England | 0–1 | Championship · England |
| 2009-03-14 | LivornovCittadellaSerie B · Italy | 1–1 | Serie B · Italy |
| 2009-03-14 | Notts CountyvAccringtonLeague Two · England | 1–1 | League Two · England |
| 2009-03-14 | MotherwellvDundee UnitedPremiership · Scotland | 2–1 | Premiership · Scotland |
| 2009-03-14 | HeerenveenvSpartaEredivisie · Netherlands | 5–1 | Eredivisie · Netherlands |
| 2009-03-14 | SouthamptonvQPRChampionship · England | 0–0 | Championship · England |
| 2009-03-14 | ExetervBradfordLeague Two · England | 1–0 | League Two · England |
| 2009-03-14 | CaenvLilleLigue 1 · France | 0–1 | Ligue 1 · France |
| 2009-03-14 | LeedsvSwindonLeague One · England | 1–0 | League One · England |
| 2009-03-14 | ForfarvAlbion RvsLeague Two · Scotland | 4–0 | League Two · Scotland |
| 2009-03-14 | MiddlesbroughvPortsmouthPremier League · England | 1–1 | Premier League · England |
| 2009-03-14 | WaregemvRoeselarePro League · Belgium | 0–0 | Pro League · Belgium |