| 2010-03-13 | NancyvBoulogneLigue 1 · France | 1–3 | Ligue 1 · France |
| 2010-03-13 | ClydevCowdenbeathLeague One · Scotland | 1–2 | League One · Scotland |
| 2010-03-13 | NumanciavHuescaLa Liga 2 · Spain | 1–0 | La Liga 2 · Spain |
| 2010-03-13 | Queens ParkvEast StirlingLeague Two · Scotland | 2–0 | League Two · Scotland |
| 2010-03-13 | NorthamptonvDarlingtonLeague Two · England | 2–0 | League Two · England |
| 2010-03-13 | HuddersfieldvNorwichLeague One · England | 1–3 | League One · England |
| 2010-03-13 | ShrewsburyvRochdaleLeague Two · England | 0–1 | League Two · England |
| 2010-03-13 | HullvArsenalPremier League · England | 1–2 | Premier League · England |
| 2010-03-13 | Sp BragavRio AvePrimeira Liga · Portugal | 1–0 | Primeira Liga · Portugal |
| 2010-03-13 | CordobavMurciaLa Liga 2 · Spain | 0–1 | La Liga 2 · Spain |
| 2010-03-13 | VVV VenlovUtrechtEredivisie · Netherlands | 0–1 | Eredivisie · Netherlands |
| 2010-03-13 | East FifevPeterheadLeague One · Scotland | 3–0 | League One · Scotland |
| 2010-03-13 | TottenhamvBlackburnPremier League · England | 3–1 | Premier League · England |
| 2010-03-13 | StockportvGillinghamLeague One · England | 0–0 | League One · England |
| 2010-03-13 | Bayern MunichvFreiburgBundesliga · Germany | 2–1 | Bundesliga · Germany |
| 2010-03-13 | AFC WimbledonvEastbourne BoroughNational League · England | 2–0 | National League · England |
| 2010-03-13 | YeovilvHartlepoolLeague One · England | 4–0 | League One · England |
| 2010-03-13 | NAC BredavGroningenEredivisie · Netherlands | 0–3 | Eredivisie · Netherlands |
| 2010-03-13 | Le MansvNiceLigue 1 · France | 0–1 | Ligue 1 · France |
| 2010-03-13 | BurtonvCheltenhamLeague Two · England | 5–6 | League Two · England |
| 2010-03-13 | M'gladbachvWolfsburgBundesliga · Germany | 0–4 | Bundesliga · Germany |
| 2010-03-13 | SassuolovAscoliSerie B · Italy | 0–1 | Serie B · Italy |
| 2010-03-13 | BoltonvWiganPremier League · England | 4–0 | Premier League · England |
| 2010-03-13 | AtromitosvIraklisSuper League · Greece | 2–1 | Super League · Greece |
| 2010-03-13 | MacclesfieldvBuryLeague Two · England | 2–0 | League Two · England |
| 2010-03-13 | FrosinonevPiacenzaSerie B · Italy | 2–3 | Serie B · Italy |
| 2010-03-13 | CambridgevHistonNational League · England | 2–1 | National League · England |
| 2010-03-13 | BradfordvAldershotLeague Two · England | 2–1 | League Two · England |
| 2010-03-13 | Inverness CvMortonChampionship · Scotland | 1–0 | Championship · Scotland |
| 2010-03-13 | CoventryvPlymouthChampionship · England | 1–1 | Championship · England |
| 2010-03-13 | BochumvDortmundBundesliga · Germany | 1–4 | Bundesliga · Germany |
| 2010-03-13 | AnconavTorinoSerie B · Italy | 2–1 | Serie B · Italy |
| 2010-03-13 | StranraervBerwickLeague Two · Scotland | 3–1 | League Two · Scotland |
| 2010-03-13 | LeicestervCardiffChampionship · England | 1–0 | Championship · England |
| 2010-03-13 | CastellonvGironaLa Liga 2 · Spain | 0–1 | La Liga 2 · Spain |
| 2010-03-13 | ChesterfieldvPort ValeLeague Two · England | 0–5 | League Two · England |
| 2010-03-13 | WatfordvPeterboroChampionship · England | 0–1 | Championship · England |
| 2010-03-13 | GenclerbirligivFenerbahceSüper Lig · Turkey | 0–0 | Süper Lig · Turkey |
| 2010-03-13 | Sheffield UnitedvQPRChampionship · England | 1–1 | Championship · England |
| 2010-03-13 | EmpolivGallipoliSerie B · Italy | 2–2 | Serie B · Italy |
| 2010-03-13 | Albion RvsvMontroseLeague Two · Scotland | 1–0 | League Two · Scotland |
| 2010-03-13 | AZ AlkmaarvWaalwijkEredivisie · Netherlands | 6–2 | Eredivisie · Netherlands |
| 2010-03-13 | PadovavCrotoneSerie B · Italy | 2–0 | Serie B · Italy |
| 2010-03-13 | HamiltonvSt MirrenPremiership · Scotland | 1–0 | Premiership · Scotland |
| 2010-03-13 | BrentfordvMilton Keynes DonsLeague One · England | 3–3 | League One · England |
| 2010-03-13 | Dag and RedvRotherhamLeague Two · England | 0–1 | League Two · England |
| 2010-03-13 | FalkirkvSt JohnstonePremiership · Scotland | 1–2 | Premiership · Scotland |
| 2010-03-13 | HerthavNurnbergBundesliga · Germany | 1–2 | Bundesliga · Germany |
| 2010-03-13 | Frankfurt FSVvFortuna Dusseldorf2. Bundesliga · Germany | 2–0 | 2. Bundesliga · Germany |
| 2010-03-13 | GraysvHayes & YeadingNational League · England | 0–0 | National League · England |