| 2010-03-14 | KortrijkvLokerenPro League · Belgium | 3–1 | Pro League · Belgium |
| 2010-03-14 | RecreativovElcheLa Liga 2 · Spain | 2–1 | La Liga 2 · Spain |
| 2010-03-14 | GenoavCagliariSerie A · Italy | 5–3 | Serie A · Italy |
| 2010-03-14 | LaziovBariSerie A · Italy | 0–2 | Serie A · Italy |
| 2010-03-14 | St TruidenvGerminalPro League · Belgium | 2–0 | Pro League · Belgium |
| 2010-03-14 | WesterlovCercle BruggePro League · Belgium | 2–1 | Pro League · Belgium |
| 2010-03-14 | HoffenheimvWerder BremenBundesliga · Germany | 0–1 | Bundesliga · Germany |
| 2010-03-14 | NacionalvBenficaPrimeira Liga · Portugal | 0–1 | Primeira Liga · Portugal |
| 2010-03-14 | ToulousevMarseilleLigue 1 · France | 1–1 | Ligue 1 · France |
| 2010-03-14 | ArisvPAOKSuper League · Greece | 2–0 | Super League · Greece |
| 2010-03-14 | LivornovRomaSerie A · Italy | 3–3 | Serie A · Italy |
| 2010-03-14 | LeixoesvSetubalPrimeira Liga · Portugal | 1–2 | Primeira Liga · Portugal |
| 2010-03-14 | BursasporvManisasporSüper Lig · Turkey | 2–0 | Süper Lig · Turkey |
| 2010-03-14 | AntalyasporvSivassporSüper Lig · Turkey | 3–0 | Süper Lig · Turkey |
| 2010-03-14 | GentvCharleroiPro League · Belgium | 2–1 | Pro League · Belgium |
| 2010-03-14 | JuventusvSienaSerie A · Italy | 3–3 | Serie A · Italy |
| 2010-03-14 | LeverkusenvHamburgBundesliga · Germany | 4–2 | Bundesliga · Germany |
| 2010-03-13 | ForfarvAnnan AthleticLeague Two · Scotland | 1–5 | League Two · Scotland |
| 2010-03-13 | MontpelliervAuxerreLigue 1 · France | 1–1 | Ligue 1 · France |
| 2010-03-13 | StirlingvAlloaLeague One · Scotland | 0–3 | League One · Scotland |
| 2010-03-13 | LevantevGimnasticLa Liga 2 · Spain | 6–1 | La Liga 2 · Spain |
| 2010-03-13 | Real UnionvBetisLa Liga 2 · Spain | 1–3 | La Liga 2 · Spain |
| 2010-03-13 | OldhamvWycombeLeague One · England | 2–2 | League One · England |
| 2010-03-13 | DoncastervDerbyChampionship · England | 2–1 | Championship · England |
| 2010-03-13 | BarnetvAccringtonLeague Two · England | 1–2 | League Two · England |
| 2010-03-13 | GetafevMallorcaLa Liga · Spain | 3–0 | La Liga · Spain |
| 2010-03-13 | LyonvSt EtienneLigue 1 · France | 1–1 | Ligue 1 · France |
| 2010-03-13 | SalernitanavCesenaSerie B · Italy | 0–0 | Serie B · Italy |
| 2010-03-13 | MorecambevGrimsbyLeague Two · England | 1–1 | League Two · England |
| 2010-03-13 | MansfieldvTamworthNational League · England | 0–0 | National League · England |
| 2010-03-13 | VallecanovVillarreal BLa Liga 2 · Spain | 0–2 | La Liga 2 · Spain |
| 2010-03-13 | MainzvFC KolnBundesliga · Germany | 1–0 | Bundesliga · Germany |
| 2010-03-13 | KarlsruhevAugsburg2. Bundesliga · Germany | 1–0 | 2. Bundesliga · Germany |
| 2010-03-13 | IpswichvScunthorpeChampionship · England | 1–0 | Championship · England |
| 2010-03-13 | NapolivFiorentinaSerie A · Italy | 1–3 | Serie A · Italy |
| 2010-03-13 | West BromvBlackpoolChampionship · England | 3–2 | Championship · England |
| 2010-03-13 | HannovervEin FrankfurtBundesliga · Germany | 2–1 | Bundesliga · Germany |
| 2010-03-13 | LorientvLensLigue 1 · France | 1–0 | Ligue 1 · France |
| 2010-03-13 | Crawley TownvEbbsfleetNational League · England | 2–1 | National League · England |
| 2010-03-13 |  MonacovBordeauxLigue 1 · France | 0–0 | Ligue 1 · France |
| 2010-03-13 | TorquayvCreweLeague Two · England | 1–1 | League Two · England |
| 2010-03-13 | SevillavLa CorunaLa Liga · Spain | 1–1 | La Liga · Spain |
| 2010-03-13 | LutonvWrexhamNational League · England | 1–0 | National League · England |
| 2010-03-13 | MotherwellvHeartsPremiership · Scotland | 3–1 | Premiership · Scotland |
| 2010-03-13 | RegginavModenaSerie B · Italy | 0–1 | Serie B · Italy |
| 2010-03-13 | Airdrie UtdvAyrChampionship · Scotland | 1–1 | Championship · Scotland |
| 2010-03-13 | BarnsleyvCrystal PalaceChampionship · England | 0–0 | Championship · England |
| 2010-03-13 | MiddlesbroughvNewcastleChampionship · England | 2–2 | Championship · England |
| 2010-03-13 | CarlislevColchesterLeague One · England | 2–1 | League One · England |
| 2010-03-13 | OlympiakosvGianninaSuper League · Greece | 2–2 | Super League · Greece |