| 2009-03-21 | AnderlechtvGerminalPro League · Belgium | 2–0 | Pro League · Belgium |
| 2009-03-21 | BarnetvLincolnLeague Two · England | 3–2 | League Two · England |
| 2009-03-21 | Las PalmasvXerezLa Liga 2 · Spain | 2–3 | La Liga 2 · Spain |
| 2009-03-21 | ModenavVicenzaSerie B · Italy | 3–1 | Serie B · Italy |
| 2009-03-21 | Queens ParkvStirlingLeague One · Scotland | 3–1 | League One · Scotland |
| 2009-03-21 | QPRvBristol CityChampionship · England | 2–1 | Championship · England |
| 2009-03-21 | BlackburnvWest HamPremier League · England | 1–1 | Premier League · England |
| 2009-03-21 | HoffenheimvHannoverBundesliga · Germany | 2–2 | Bundesliga · Germany |
| 2009-03-21 | NijmegenvWillem IIEredivisie · Netherlands | 0–0 | Eredivisie · Netherlands |
| 2009-03-21 | OldhamvTranmereLeague One · England | 0–2 | League One · England |
| 2009-03-21 | PlymouthvBurnleyChampionship · England | 1–2 | Championship · England |
| 2009-03-21 | StokevMiddlesbroughPremier League · England | 1–0 | Premier League · England |
| 2009-03-21 | RomavJuventusSerie A · Italy | 1–4 | Serie A · Italy |
| 2009-03-21 | PSV EindhovenvVitesseEredivisie · Netherlands | 2–0 | Eredivisie · Netherlands |
| 2009-03-21 | BerwickvElginLeague Two · Scotland | 2–1 | League Two · Scotland |
| 2009-03-21 | FulhamvMan UnitedPremier League · England | 2–0 | Premier League · England |
| 2009-03-21 | Eastbourne BoroughvBurtonNational League · England | 1–2 | National League · England |
| 2009-03-21 | BielefeldvWolfsburgBundesliga · Germany | 0–3 | Bundesliga · Germany |
| 2009-03-21 | TriestinavParmaSerie B · Italy | 0–3 | Serie B · Italy |
| 2009-03-21 | CottbusvFC KolnBundesliga · Germany | 0–2 | Bundesliga · Germany |
| 2009-03-21 | Albion RvsvMontroseLeague Two · Scotland | 0–1 | League Two · Scotland |
| 2009-03-21 | CordobavEibarLa Liga 2 · Spain | 1–1 | La Liga 2 · Spain |
| 2009-03-21 | WesterlovDenderPro League · Belgium | 3–1 | Pro League · Belgium |
| 2009-03-21 | West BromvBoltonPremier League · England | 1–1 | Premier League · England |
| 2009-03-21 | Cercle BruggevMouscronPro League · Belgium | 0–1 | Pro League · Belgium |
| 2009-03-21 | ClydevDunfermlineChampionship · Scotland | 1–4 | Championship · Scotland |
| 2009-03-21 | Queen of SthvLivingstonChampionship · Scotland | 3–3 | Championship · Scotland |
| 2009-03-21 | RennesvValenciennesLigue 1 · France | 0–0 | Ligue 1 · France |
| 2009-03-21 | HuddersfieldvCheltenhamLeague One · England | 2–2 | League One · England |
| 2009-03-21 | SalisburyvRushden & DNational League · England | 1–1 | National League · England |
| 2009-03-21 | ScunthorpevBrightonLeague One · England | 2–0 | League One · England |
| 2009-03-21 | KayserisporvAnkaragucuSüper Lig · Turkey | 2–0 | Süper Lig · Turkey |
| 2009-03-21 | SevillavValladolidLa Liga · Spain | 4–1 | La Liga · Spain |
| 2009-03-21 | MurciavAlavesLa Liga 2 · Spain | 1–0 | La Liga 2 · Spain |
| 2009-03-21 | BergenvLokerenPro League · Belgium | 0–2 | Pro League · Belgium |
| 2009-03-21 | ShrewsburyvMorecambeLeague Two · England | 0–0 | League Two · England |
| 2009-03-21 | Ross CountyvMortonChampionship · Scotland | 1–1 | Championship · Scotland |
| 2009-03-21 | GenkvTubizePro League · Belgium | 3–0 | Pro League · Belgium |
| 2009-03-21 | PiacenzavFrosinoneSerie B · Italy | 3–0 | Serie B · Italy |
| 2009-03-21 | HamiltonvSt MirrenPremiership · Scotland | 0–0 | Premiership · Scotland |
| 2009-03-21 | LillevGrenobleLigue 1 · France | 2–1 | Ligue 1 · France |
| 2009-03-21 | TottenhamvChelseaPremier League · England | 1–0 | Premier League · England |
| 2009-03-21 | DumbartonvAnnan AthleticLeague Two · Scotland | 0–2 | League Two · Scotland |
| 2009-03-21 | AlicantevCeltaLa Liga 2 · Spain | 2–2 | La Liga 2 · Spain |
| 2009-03-21 | MarseillevNantesLigue 1 · France | 2–0 | Ligue 1 · France |
| 2009-03-21 | YeovilvMilton Keynes DonsLeague One · England | 0–0 | League One · England |
| 2009-03-21 | VillarrealvAth BilbaoLa Liga · Spain | 2–0 | La Liga · Spain |
| 2009-03-21 | LevantevCastellonLa Liga 2 · Spain | 3–1 | La Liga 2 · Spain |
| 2009-03-21 | HartlepoolvMillwallLeague One · England | 2–3 | League One · England |
| 2009-03-21 | AlbacetevHuescaLa Liga 2 · Spain | 0–2 | La Liga 2 · Spain |