| 2009-03-21 | IpswichvWatfordChampionship · England | 0–0 | Championship · England |
| 2009-03-21 | KocaelisporvAnkarasporSüper Lig · Turkey | 3–1 | Süper Lig · Turkey |
| 2009-03-21 | Leyton OrientvWalsallLeague One · England | 0–1 | League One · England |
| 2009-03-21 | KidderminstervWokingNational League · England | 3–0 | National League · England |
| 2009-03-21 | Le HavrevBordeauxLigue 1 · France | 0–3 | Ligue 1 · France |
| 2009-03-21 | BarivCittadellaSerie B · Italy | 2–0 | Serie B · Italy |
| 2009-03-21 | NicevCaenLigue 1 · France | 2–2 | Ligue 1 · France |
| 2009-03-21 | SouthendvCarlisleLeague One · England | 3–0 | League One · England |
| 2009-03-21 | CataniavLazioSerie A · Italy | 1–0 | Serie A · Italy |
| 2009-03-21 | PortsmouthvEvertonPremier League · England | 2–1 | Premier League · England |
| 2009-03-21 | RotherhamvChesterfieldLeague Two · England | 3–0 | League Two · England |
| 2009-03-21 | ArbroathvRaith RvsLeague One · Scotland | 0–2 | League One · Scotland |
| 2009-03-21 | NorthwichvTorquayNational League · England | 2–3 | National League · England |
| 2009-03-21 | LivornovAnconaSerie B · Italy | 2–3 | Serie B · Italy |
| 2009-03-21 | CoventryvDoncasterChampionship · England | 1–0 | Championship · England |
| 2009-03-21 | GrimsbyvGillinghamLeague Two · England | 3–0 | League Two · England |
| 2009-03-21 | BournemouthvNotts CountyLeague Two · England | 0–1 | League Two · England |
| 2009-03-21 | MantovavRiminiSerie B · Italy | 1–0 | Serie B · Italy |
| 2009-03-21 | SivassporvBesiktasSüper Lig · Turkey | 1–1 | Süper Lig · Turkey |
| 2009-03-21 | AlderneyvGuernseyMuratti Vase | 0–5 | Muratti Vase |
| 2009-03-21 | AccringtonvExeterLeague Two · England | 2–1 | League Two · England |
| 2009-03-21 | Nancyv MonacoLigue 1 · France | 0–1 | Ligue 1 · France |
| 2009-03-21 | AvellinovPisaSerie B · Italy | 2–0 | Serie B · Italy |
| 2009-03-21 | BlackpoolvSouthamptonChampionship · England | 1–1 | Championship · England |
| 2009-03-21 | East FifevStranraerLeague One · Scotland | 4–0 | League One · Scotland |
| 2009-03-21 | CharltonvPrestonChampionship · England | 0–0 | Championship · England |
| 2009-03-21 | KilmarnockvMotherwellPremiership · Scotland | 0–0 | Premiership · Scotland |
| 2009-03-21 | StenhousemuirvForfarLeague Two · Scotland | 0–1 | League Two · Scotland |
| 2009-03-21 | WrexhamvCrawley TownNational League · England | 0–2 | National League · England |
| 2009-03-21 | LeicestervColchesterLeague One · England | 1–1 | League One · England |
| 2009-03-21 | AscolivAlbinoleffeSerie B · Italy | 2–2 | Serie B · Italy |
| 2009-03-21 | BuyuksehyrvKonyasporSüper Lig · Turkey | 2–0 | Süper Lig · Turkey |
| 2009-03-21 | FalkirkvInverness CPremiership · Scotland | 4–0 | Premiership · Scotland |
| 2009-03-21 | BirminghamvNorwichChampionship · England | 1–1 | Championship · England |
| 2009-03-21 | St JohnstonevAirdrie UtdChampionship · Scotland | 3–0 | Championship · Scotland |
| 2009-03-21 | EmpolivSassuoloSerie B · Italy | 3–2 | Serie B · Italy |
| 2009-03-21 | NorthamptonvStockportLeague One · England | 4–0 | League One · England |
| 2009-03-21 | HacettepesporvAntalyasporSüper Lig · Turkey | 1–2 | Süper Lig · Turkey |
| 2009-03-21 | SalamancavTenerifeLa Liga 2 · Spain | 1–2 | La Liga 2 · Spain |
| 2009-03-21 | HibernianvAberdeenPremiership · Scotland | 0–0 | Premiership · Scotland |
| 2009-03-21 | RoeselarevMechelenPro League · Belgium | 1–0 | Pro League · Belgium |
| 2009-03-21 | NewcastlevArsenalPremier League · England | 1–3 | Premier League · England |
| 2009-03-21 | ZaragozavSevilla BLa Liga 2 · Spain | 4–0 | La Liga 2 · Spain |
| 2009-03-21 | GraysvMansfieldNational League · England | 2–1 | National League · England |
| 2009-03-21 | GaziantepsporvTrabzonsporSüper Lig · Turkey | 3–2 | Süper Lig · Turkey |
| 2009-03-21 | DarlingtonvAldershotLeague Two · England | 2–0 | League Two · England |
| 2009-03-21 | SpartavHeraclesEredivisie · Netherlands | 1–0 | Eredivisie · Netherlands |
| 2009-03-21 | LeverkusenvEin FrankfurtBundesliga · Germany | 1–1 | Bundesliga · Germany |
| 2009-03-21 | StuttgartvHerthaBundesliga · Germany | 2–0 | Bundesliga · Germany |
| 2009-03-21 | BarrowvLewesNational League · England | 2–0 | National League · England |