| 2010-03-20 | Dag and RedvMacclesfieldLeague Two · England | 3–1 | League Two · England |
| 2010-03-20 | CeltavCartagenaLa Liga 2 · Spain | 1–0 | La Liga 2 · Spain |
| 2010-03-20 | PSV EindhovenvTwenteEredivisie · Netherlands | 1–1 | Eredivisie · Netherlands |
| 2010-03-20 | BoulognevLorientLigue 1 · France | 2–0 | Ligue 1 · France |
| 2010-03-20 | FreiburgvMainzBundesliga · Germany | 1–0 | Bundesliga · Germany |
| 2010-03-20 | Eastbourne BoroughvGraysNational League · England | 2–2 | National League · England |
| 2010-03-20 | Werder BremenvBochumBundesliga · Germany | 3–2 | Bundesliga · Germany |
| 2010-03-20 | TriestinavEmpoliSerie B · Italy | 1–0 | Serie B · Italy |
| 2010-03-20 | LensvSochauxLigue 1 · France | 0–0 | Ligue 1 · France |
| 2010-03-20 | SwindonvNorwichLeague One · England | 1–1 | League One · England |
| 2010-03-20 | Annan AthleticvQueens ParkLeague Two · Scotland | 0–2 | League Two · Scotland |
| 2010-03-20 | SunderlandvBirminghamPremier League · England | 3–1 | Premier League · England |
| 2010-03-20 | AccringtonvRochdaleLeague Two · England | 2–4 | League Two · England |
| 2010-03-20 | FiorentinavGenoaSerie A · Italy | 3–0 | Serie A · Italy |
| 2010-03-20 | NicevParis SGLigue 1 · France | 1–0 | Ligue 1 · France |
| 2010-03-20 | DortmundvLeverkusenBundesliga · Germany | 3–0 | Bundesliga · Germany |
| 2010-03-20 | DoncastervSheffield UnitedChampionship · England | 1–1 | Championship · England |
| 2010-03-20 | StockportvBrentfordLeague One · England | 0–1 | League One · England |
| 2010-03-20 | Willem IIvUtrechtEredivisie · Netherlands | 0–3 | Eredivisie · Netherlands |
| 2010-03-20 | St EtiennevNancyLigue 1 · France | 0–0 | Ligue 1 · France |
| 2010-03-20 | Ein FrankfurtvBayern MunichBundesliga · Germany | 2–1 | Bundesliga · Germany |
| 2010-03-20 | CrewevNotts CountyLeague Two · England | 0–1 | League Two · England |
| 2010-03-20 | HistonvMansfieldNational League · England | 0–5 | National League · England |
| 2010-03-20 | NorthamptonvMorecambeLeague Two · England | 2–0 | League Two · England |
| 2010-03-20 | Ross CountyvInverness CChampionship · Scotland | 0–0 | Championship · Scotland |
| 2010-03-20 | MortonvPartickChampionship · Scotland | 1–0 | Championship · Scotland |
| 2010-03-20 | Villarreal BvReal UnionLa Liga 2 · Spain | 3–1 | La Liga 2 · Spain |
| 2010-03-20 | EspanolvSevillaLa Liga · Spain | 2–0 | La Liga · Spain |
| 2010-03-20 | XerezvTenerifeLa Liga · Spain | 2–1 | La Liga · Spain |
| 2010-03-20 | IpswichvBarnsleyChampionship · England | 1–0 | Championship · England |
| 2010-03-20 | CelticvSt JohnstonePremiership · Scotland | 3–0 | Premiership · Scotland |
| 2010-03-20 | HerefordvBradfordLeague Two · England | 2–0 | League Two · England |
| 2010-03-20 | Nott'm ForestvPeterboroChampionship · England | 1–0 | Championship · England |
| 2010-03-20 | GrimsbyvBournemouthLeague Two · England | 3–2 | League Two · England |
| 2010-03-20 | East FifevStirlingLeague One · Scotland | 0–3 | League One · Scotland |
| 2010-03-20 | Airdrie UtdvDundeeChampionship · Scotland | 3–0 | Championship · Scotland |
| 2010-03-20 | CesenavVicenzaSerie B · Italy | 3–1 | Serie B · Italy |
| 2010-03-20 | AlloavStenhousemuirLeague One · Scotland | 2–1 | League One · Scotland |
| 2010-03-20 | HeraclesvNAC BredaEredivisie · Netherlands | 3–0 | Eredivisie · Netherlands |
| 2010-03-20 | ForfarvAlbion RvsLeague Two · Scotland | 1–1 | League Two · Scotland |
| 2010-03-20 | OldhamvBrightonLeague One · England | 0–2 | League One · England |
| 2010-03-20 | AberdeenvDundee UnitedPremiership · Scotland | 2–2 | Premiership · Scotland |
| 2010-03-20 | WrexhamvCambridgeNational League · England | 2–2 | National League · England |
| 2010-03-20 | LivingstonvStranraerLeague Two · Scotland | 2–1 | League Two · Scotland |
| 2010-03-20 | YeovilvBristol RvsLeague One · England | 0–3 | League One · England |
| 2010-03-20 | CharltonvGillinghamLeague One · England | 2–2 | League One · England |
| 2010-03-20 | StuttgartvHannoverBundesliga · Germany | 2–0 | Bundesliga · Germany |
| 2010-03-20 | Raith RvsvDunfermlineChampionship · Scotland | 1–2 | Championship · Scotland |
| 2010-03-20 | ModenavTorinoSerie B · Italy | 0–2 | Serie B · Italy |
| 2010-03-20 | CrotonevFrosinoneSerie B · Italy | 3–0 | Serie B · Italy |