| 2021-08-21 | OdensevBrondbySuperliga · Denmark | 2–2 | Superliga · Denmark |
| 2021-08-21 | Columbus CrewvSeattle SoundersMLS · United States | 1–2 | MLS · United States |
| 2021-08-21 | Le HavrevNiortLigue 2 · France | 2–1 | Ligue 2 · France |
| 2021-08-21 | Los Angeles GalaxyvSan Jose EarthquakesMLS · United States | 1–2 | MLS · United States |
| 2021-08-21 | EmpolivLazioSerie A · Italy | 1–3 | Serie A · Italy |
| 2021-08-21 | St. GallenvSionSuper League · Switzerland | 1–1 | Super League · Switzerland |
| 2021-08-21 | MansfieldvBradfordLeague Two · England | 2–3 | League Two · England |
| 2021-08-21 | GillinghamvMorecambeLeague One · England | 2–1 | League One · England |
| 2021-08-21 | BochumvMainzBundesliga · Germany | 2–0 | Bundesliga · Germany |
| 2021-08-21 | Forest GreenvCrawley TownLeague Two · England | 6–3 | League Two · England |
| 2021-08-21 | TorinovAtalantaSerie A · Italy | 1–2 | Serie A · Italy |
| 2021-08-21 |  MonacovLensLigue 1 · France | 0–2 | Ligue 1 · France |
| 2021-08-21 | ShrewsburyvPlymouthLeague One · England | 0–3 | League One · England |
| 2021-08-21 | CambridgevBurtonLeague One · England | 3–0 | League One · England |
| 2021-08-21 | BlackburnvWest BromChampionship · England | 1–2 | Championship · England |
| 2021-08-21 | Univ. CraiovavGaz Metan MediasSuperliga · Romania | 1–0 | Superliga · Romania |
| 2021-08-21 | Greuther FurthvBielefeldBundesliga · Germany | 1–1 | Bundesliga · Germany |
| 2021-08-21 | Gamba OsakavFC TokyoJ1 League · Japan | 0–0 | J1 League · Japan |
| 2021-08-21 | RegensburgvSchalke 042. Bundesliga · Germany | 4–1 | 2. Bundesliga · Germany |
| 2021-08-21 | Velez SarsfieldvLanusLiga Profesional · Argentina | 5–1 | Liga Profesional · Argentina |
| 2021-08-21 | SalfordvSwindonLeague Two · England | 0–1 | League Two · England |
| 2021-08-21 | AlanyasporvAltaySüper Lig · Turkey | 1–4 | Süper Lig · Turkey |
| 2021-08-21 | NorthamptonvRochdaleLeague Two · England | 1–3 | League Two · England |
| 2021-08-21 | IntervGenoaSerie A · Italy | 4–0 | Serie A · Italy |
| 2021-08-21 | VeronavSassuoloSerie A · Italy | 2–3 | Serie A · Italy |
| 2021-08-21 | Ein FrankfurtvAugsburgBundesliga · Germany | 0–0 | Bundesliga · Germany |
| 2021-08-21 | Atletico GOvChapecoense-SCSerie A · Brazil | 1–1 | Serie A · Brazil |
| 2021-08-21 | HeerenveenvWaalwijkEredivisie · Netherlands | 3–2 | Eredivisie · Netherlands |
| 2021-08-21 | Arsenal SarandivRacing ClubLiga Profesional · Argentina | 0–3 | Liga Profesional · Argentina |
| 2021-08-21 | Mazatlan FCvTigres UANLLiga MX · Mexico | 0–3 | Liga MX · Mexico |
| 2021-08-21 | KarlsruhevWerder Bremen2. Bundesliga · Germany | 0–0 | 2. Bundesliga · Germany |
| 2021-08-21 | GranadavValenciaLa Liga · Spain | 1–1 | La Liga · Spain |
| 2021-08-21 | DunkerquevNimesLigue 2 · France | 0–2 | Ligue 2 · France |
| 2021-08-21 | HamiltonvKilmarnockChampionship · Scotland | 0–2 | Championship · Scotland |
| 2021-08-21 | HartlepoolvWalsallLeague Two · England | 2–0 | League Two · England |
| 2021-08-21 | Cerezo OsakavYokohama FCJ1 League · Japan | 3–1 | J1 League · Japan |
| 2021-08-21 | BarnetvNotts CountyNational League · England | 0–5 | National League · England |
| 2021-08-21 | LiverpoolvBurnleyPremier League · England | 2–0 | Premier League · England |
| 2021-08-21 | Kashiwa ReysolvSagan TosuJ1 League · Japan | 1–3 | J1 League · Japan |
| 2021-08-21 | Inverness CvAyrChampionship · Scotland | 1–0 | Championship · Scotland |
| 2021-08-21 | StranraervStirlingLeague Two · Scotland | 0–3 | League Two · Scotland |
| 2021-08-21 | Kelty HeartsvEdinburgh CityLeague Two · Scotland | 1–0 | League Two · Scotland |
| 2021-08-21 | StevenagevPort ValeLeague Two · England | 1–1 | League Two · England |
| 2021-08-21 | Ath BilbaovBarcelonaLa Liga · Spain | 1–1 | La Liga · Spain |
| 2021-08-21 | SalzburgvA. KlagenfurtBundesliga · Austria | 3–1 | Bundesliga · Austria |
| 2021-08-21 | RotherhamvSheffield WedsLeague One · England | 0–2 | League One · England |
| 2021-08-21 | SeraingvCercle BruggePro League · Belgium | 2–1 | Pro League · Belgium |
| 2021-08-21 | ElginvForfarLeague Two · Scotland | 1–1 | League Two · Scotland |
| 2021-08-21 | LivingstonvMotherwellPremiership · Scotland | 1–2 | Premiership · Scotland |
| 2021-08-21 | Arsenal TulavSpartak MoscowPremier League · Russia | 1–1 | Premier League · Russia |