| 2010-03-27 | AlbinoleffevBresciaSerie B · Italy | 1–1 | Serie B · Italy |
| 2010-03-27 | BarnsleyvDoncasterChampionship · England | 0–1 | Championship · England |
| 2010-03-27 | StranraervEast StirlingLeague Two · Scotland | 2–2 | League Two · Scotland |
| 2010-03-27 | Raith RvsvAyrChampionship · Scotland | 1–1 | Championship · Scotland |
| 2010-03-27 | BournemouthvAccringtonLeague Two · England | 2–0 | League Two · England |
| 2010-03-27 | Notts CountyvRotherhamLeague Two · England | 1–0 | League Two · England |
| 2010-03-27 | ZaragozavValenciaLa Liga · Spain | 3–0 | La Liga · Spain |
| 2010-03-27 | PartickvInverness CChampionship · Scotland | 0–1 | Championship · Scotland |
| 2010-03-27 | ElginvForfarLeague Two · Scotland | 0–2 | League Two · Scotland |
| 2010-03-27 | Bristol RvsvSouthendLeague One · England | 4–3 | League One · England |
| 2010-03-27 | Roda JCvHeerenveenEredivisie · Netherlands | 4–2 | Eredivisie · Netherlands |
| 2010-03-27 | MantovavVicenzaSerie B · Italy | 1–0 | Serie B · Italy |
| 2010-03-27 | GenclerbirligivDiyarbakirsporSüper Lig · Turkey | 1–0 | Süper Lig · Turkey |
| 2010-03-27 | Ross CountyvDundeeChampionship · Scotland | 1–1 | Championship · Scotland |
| 2010-03-27 | CelticvKilmarnockPremiership · Scotland | 3–1 | Premiership · Scotland |
| 2010-03-27 | ColchestervWycombeLeague One · England | 1–1 | League One · England |
| 2010-03-27 | Queens ParkvLivingstonLeague Two · Scotland | 0–1 | League Two · Scotland |
| 2010-03-27 | HartlepoolvSwindonLeague One · England | 0–1 | League One · England |
| 2010-03-27 | PeterborovBristol CityChampionship · England | 0–1 | Championship · England |
| 2010-03-27 | VitessevWillem IIEredivisie · Netherlands | 2–1 | Eredivisie · Netherlands |
| 2010-03-27 | NorwichvLeedsLeague One · England | 1–0 | League One · England |
| 2010-03-27 | RomavInterSerie A · Italy | 2–1 | Serie A · Italy |
| 2010-03-27 | YorkvTamworthNational League · England | 1–1 | National League · England |
| 2010-03-27 | GuimaraesvAcademicaPrimeira Liga · Portugal | 1–0 | Primeira Liga · Portugal |
| 2010-03-27 | HeartsvRangersPremiership · Scotland | 1–4 | Premiership · Scotland |
| 2010-03-27 | TwentevSpartaEredivisie · Netherlands | 3–0 | Eredivisie · Netherlands |
| 2010-03-27 | SwanseavIpswichChampionship · England | 0–0 | Championship · England |
| 2010-03-27 | CastellonvVillarreal BLa Liga 2 · Spain | 3–2 | La Liga 2 · Spain |
| 2010-03-27 | VallecanovCeltaLa Liga 2 · Spain | 1–2 | La Liga 2 · Spain |
| 2010-03-27 | ReadingvWest BromChampionship · England | 1–1 | Championship · England |
| 2010-03-27 | BoltonvMan UnitedPremier League · England | 0–4 | Premier League · England |
| 2010-03-27 | Crystal PalacevCardiffChampionship · England | 1–2 | Championship · England |
| 2010-03-27 | StirlingvCowdenbeathLeague One · Scotland | 1–0 | League One · Scotland |
| 2010-03-27 | MacclesfieldvCheltenhamLeague Two · England | 1–0 | League Two · England |
| 2010-03-27 | Queen of SthvDunfermlineChampionship · Scotland | 2–0 | Championship · Scotland |
| 2010-03-27 | LeccevGallipoliSerie B · Italy | 1–0 | Serie B · Italy |
| 2010-03-27 | CambridgevGraysNational League · England | 3–0 | National League · England |
| 2010-03-27 | FrosinonevAnconaSerie B · Italy | 1–1 | Serie B · Italy |
| 2010-03-27 | CittadellavSassuoloSerie B · Italy | 0–0 | Serie B · Italy |
| 2010-03-27 | CrotonevSalernitanaSerie B · Italy | 2–0 | Serie B · Italy |
| 2010-03-27 | HerthavDortmundBundesliga · Germany | 0–0 | Bundesliga · Germany |
| 2010-03-27 | AlbacetevLas PalmasLa Liga 2 · Spain | 3–2 | La Liga 2 · Spain |
| 2010-03-27 | BrentfordvLeyton OrientLeague One · England | 1–0 | League One · England |
| 2010-03-27 | WatfordvMiddlesbroughChampionship · England | 1–1 | Championship · England |
| 2010-03-27 | KidderminstervSalisburyNational League · England | 0–1 | National League · England |
| 2010-03-27 | Werder BremenvNurnbergBundesliga · Germany | 4–2 | Bundesliga · Germany |
| 2010-03-27 | AFC WimbledonvRushden & DNational League · England | 0–1 | National League · England |
| 2010-03-27 | AZ AlkmaarvHeraclesEredivisie · Netherlands | 3–2 | Eredivisie · Netherlands |
| 2010-03-27 | LincolnvNorthamptonLeague Two · England | 1–1 | League Two · England |
| 2010-03-27 | DumbartonvPeterheadLeague One · Scotland | 1–3 | League One · Scotland |