| 2009-04-04 | MotherwellvAberdeenPremiership · Scotland | 1–1 | Premiership · Scotland |
| 2009-04-04 | SwindonvCreweLeague One · England | 0–0 | League One · England |
| 2009-04-04 | AlmeriavVillarrealLa Liga · Spain | 3–0 | La Liga · Spain |
| 2009-04-04 | SalernitanavTrevisoSerie B · Italy | 2–2 | Serie B · Italy |
| 2009-04-04 | DarlingtonvMorecambeLeague Two · England | 0–0 | League Two · England |
| 2009-04-04 | Le MansvLyonLigue 1 · France | 1–3 | Ligue 1 · France |
| 2009-04-04 | NantesvLilleLigue 1 · France | 0–2 | Ligue 1 · France |
| 2009-04-04 | BoltonvMiddlesbroughPremier League · England | 4–1 | Premier League · England |
| 2009-04-04 | BarrowvWokingNational League · England | 0–1 | National League · England |
| 2009-04-04 | Ein FrankfurtvCottbusBundesliga · Germany | 2–1 | Bundesliga · Germany |
| 2009-04-04 |  MonacovLe HavreLigue 1 · France | 0–1 | Ligue 1 · France |
| 2009-04-04 | HartlepoolvHerefordLeague One · England | 4–2 | League One · England |
| 2009-04-04 | AvellinovAnconaSerie B · Italy | 3–0 | Serie B · Italy |
| 2009-04-04 | FulhamvLiverpoolPremier League · England | 0–1 | Premier League · England |
| 2009-04-04 | AldershotvRotherhamLeague Two · England | 0–1 | League Two · England |
| 2009-04-04 | SpartavPSV EindhovenEredivisie · Netherlands | 0–2 | Eredivisie · Netherlands |
| 2009-04-04 | ShrewsburyvGrimsbyLeague Two · England | 1–1 | League Two · England |
| 2009-04-04 | SouthamptonvCharltonChampionship · England | 2–3 | Championship · England |
| 2009-04-04 | MechelenvKortrijkPro League · Belgium | 1–1 | Pro League · Belgium |
| 2009-04-04 | Inverness CvSt MirrenPremiership · Scotland | 2–1 | Premiership · Scotland |
| 2009-04-04 | TubizevRoeselarePro League · Belgium | 0–1 | Pro League · Belgium |
| 2009-04-04 | ArsenalvMan CityPremier League · England | 2–0 | Premier League · England |
| 2009-04-04 | BesiktasvKayserisporSüper Lig · Turkey | 1–0 | Süper Lig · Turkey |
| 2009-04-04 | HullvPortsmouthPremier League · England | 0–0 | Premier League · England |
| 2009-04-04 | WaregemvCercle BruggePro League · Belgium | 3–1 | Pro League · Belgium |
| 2009-04-04 | GuimaraesvPortoPrimeira Liga · Portugal | 1–3 | Primeira Liga · Portugal |
| 2009-04-04 | VallecanovTenerifeLa Liga 2 · Spain | 0–0 | La Liga 2 · Spain |
| 2009-04-04 | AlloavPeterheadLeague One · Scotland | 1–2 | League One · Scotland |
| 2009-04-04 | ZaragozavGironaLa Liga 2 · Spain | 1–0 | La Liga 2 · Spain |
| 2009-04-04 | LewesvAltrinchamNational League · England | 2–0 | National League · England |
| 2009-04-04 | Bristol CityvPrestonChampionship · England | 1–1 | Championship · England |
| 2009-04-04 | St JohnstonevPartickChampionship · Scotland | 1–1 | Championship · Scotland |
| 2009-04-04 | BournemouthvRochdaleLeague Two · England | 4–0 | League Two · England |
| 2009-04-04 | MillwallvWalsallLeague One · England | 3–1 | League One · England |
| 2009-04-04 | West HamvSunderlandPremier League · England | 2–0 | Premier League · England |
| 2009-04-04 | BrechinvQueens ParkLeague One · Scotland | 2–0 | League One · Scotland |
| 2009-04-04 | BetisvNumanciaLa Liga · Spain | 3–3 | La Liga · Spain |
| 2009-04-04 | GillinghamvBarnetLeague Two · England | 0–2 | League Two · England |
| 2009-04-04 | ValladolidvBarcelonaLa Liga · Spain | 0–1 | La Liga · Spain |
| 2009-04-04 | HerthavDortmundBundesliga · Germany | 1–3 | Bundesliga · Germany |
| 2009-04-04 | CowdenbeathvElginLeague Two · Scotland | 1–1 | League Two · Scotland |
| 2009-04-04 | UtrechtvGroningenEredivisie · Netherlands | 0–1 | Eredivisie · Netherlands |
| 2009-04-04 | Port ValevBuryLeague Two · England | 1–1 | League Two · England |
| 2009-04-04 | StenhousemuirvDumbartonLeague Two · Scotland | 0–2 | League Two · Scotland |
| 2009-04-04 | LivornovVicenzaSerie B · Italy | 1–1 | Serie B · Italy |
| 2009-04-04 | WrexhamvMansfieldNational League · England | 2–0 | National League · England |
| 2009-04-04 | ValenciennesvAuxerreLigue 1 · France | 2–0 | Ligue 1 · France |
| 2009-04-04 | AyrvStirlingLeague One · Scotland | 3–1 | League One · Scotland |
| 2009-04-04 | West BromvStokePremier League · England | 0–2 | Premier League · England |
| 2009-04-04 | HeartsvKilmarnockPremiership · Scotland | 3–1 | Premiership · Scotland |