| 2010-03-28 | DenizlisporvKasimpasaSüper Lig · Turkey | 3–3 | Süper Lig · Turkey |
| 2010-03-28 | Asteras TripolisvPanthrakikosSuper League · Greece | 3–1 | Super League · Greece |
| 2010-03-28 | GaziantepsporvSivassporSüper Lig · Turkey | 2–2 | Süper Lig · Turkey |
| 2010-03-28 | RegginavTorinoSerie B · Italy | 1–2 | Serie B · Italy |
| 2010-03-28 | SampdoriavCagliariSerie A · Italy | 1–1 | Serie A · Italy |
| 2010-03-28 | LeixoesvNavalPrimeira Liga · Portugal | 1–0 | Primeira Liga · Portugal |
| 2010-03-28 | M'gladbachvHamburgBundesliga · Germany | 1–0 | Bundesliga · Germany |
| 2010-03-28 | LarisavPAOKSuper League · Greece | 2–1 | Super League · Greece |
| 2010-03-28 | LorientvSt EtienneLigue 1 · France | 4–0 | Ligue 1 · France |
| 2010-03-28 | GalatasarayvFenerbahceSüper Lig · Turkey | 0–1 | Süper Lig · Turkey |
| 2010-03-28 | Sheffield UnitedvScunthorpeChampionship · England | 0–1 | Championship · England |
| 2010-03-28 | FiorentinavUdineseSerie A · Italy | 4–1 | Serie A · Italy |
| 2010-03-28 | SetubalvNacionalPrimeira Liga · Portugal | 2–1 | Primeira Liga · Portugal |
| 2010-03-28 | ToulousevNiceLigue 1 · France | 0–2 | Ligue 1 · France |
| 2010-03-28 | CartagenavElcheLa Liga 2 · Spain | 0–2 | La Liga 2 · Spain |
| 2010-03-28 | Real MadridvAth MadridLa Liga · Spain | 3–2 | La Liga · Spain |
| 2010-03-28 | LeiriavPacos FerreiraPrimeira Liga · Portugal | 2–1 | Primeira Liga · Portugal |
| 2010-03-28 | BelenensesvPortoPrimeira Liga · Portugal | 0–3 | Primeira Liga · Portugal |
| 2010-03-27 | ModenavPiacenzaSerie B · Italy | 0–1 | Serie B · Italy |
| 2010-03-27 | BarrowvEastbourne BoroughNational League · England | 3–2 | National League · England |
| 2010-03-27 | ClydevEast FifeLeague One · Scotland | 2–1 | League One · Scotland |
| 2010-03-27 | DerbyvLeicesterChampionship · England | 1–0 | Championship · England |
| 2010-03-27 | MontrosevBerwickLeague Two · Scotland | 1–1 | League Two · Scotland |
| 2010-03-27 | MainzvWolfsburgBundesliga · Germany | 0–2 | Bundesliga · Germany |
| 2010-03-27 | WrexhamvHistonNational League · England | 3–0 | National League · England |
| 2010-03-27 | Crawley TownvStevenageNational League · England | 0–3 | National League · England |
| 2010-03-27 | ArbroathvAlloaLeague One · Scotland | 0–0 | League One · Scotland |
| 2010-03-27 | BarnetvBurtonLeague Two · England | 1–1 | League Two · England |
| 2010-03-27 | RecreativovMurciaLa Liga 2 · Spain | 0–2 | La Liga 2 · Spain |
| 2010-03-27 | HuddersfieldvCharltonLeague One · England | 1–1 | League One · England |
| 2010-03-27 | CottbusvBielefeld2. Bundesliga · Germany | 4–1 | 2. Bundesliga · Germany |
| 2010-03-27 | Port ValevCreweLeague Two · England | 0–1 | League Two · England |
| 2010-03-27 | LeverkusenvSchalke 04Bundesliga · Germany | 0–2 | Bundesliga · Germany |
| 2010-03-27 | BradfordvDag and RedLeague Two · England | 3–3 | League Two · England |
| 2010-03-27 | Forest GreenvAltrinchamNational League · England | 4–3 | National League · England |
| 2010-03-27 | WolvesvEvertonPremier League · England | 0–0 | Premier League · England |
| 2010-03-27 | GillinghamvMilton Keynes DonsLeague One · England | 2–2 | League One · England |
| 2010-03-27 | TottenhamvPortsmouthPremier League · England | 2–0 | Premier League · England |
| 2010-03-27 | AscolivEmpoliSerie B · Italy | 2–1 | Serie B · Italy |
| 2010-03-27 | ExetervWalsallLeague One · England | 2–1 | League One · England |
| 2010-03-27 | MalagavTenerifeLa Liga · Spain | 1–1 | La Liga · Spain |
| 2010-03-27 | BrightonvTranmereLeague One · England | 3–0 | League One · England |
| 2010-03-27 | MallorcavBarcelonaLa Liga · Spain | 0–1 | La Liga · Spain |
| 2010-03-27 | Dundee UnitedvMotherwellPremiership · Scotland | 3–0 | Premiership · Scotland |
| 2010-03-27 | BuryvAldershotLeague Two · England | 1–2 | League Two · England |
| 2010-03-27 | GrossetovCesenaSerie B · Italy | 1–1 | Serie B · Italy |
| 2010-03-27 | BesiktasvEskisehirsporSüper Lig · Turkey | 3–2 | Süper Lig · Turkey |
| 2010-03-27 | AberdeenvSt MirrenPremiership · Scotland | 2–1 | Premiership · Scotland |
| 2010-03-27 | Airdrie UtdvMortonChampionship · Scotland | 3–0 | Championship · Scotland |
| 2010-03-27 | HullvFulhamPremier League · England | 2–0 | Premier League · England |