| 2010-03-30 | Kettering TownvRushden & DNational League · England | 0–3 | National League · England |
| 2010-03-30 | CardiffvLeicesterChampionship · England | 2–1 | Championship · England |
| 2010-03-30 | LutonvSalisburyNational League · England | 4–0 | National League · England |
| 2010-03-30 | Eastbourne BoroughvGatesheadNational League · England | 2–1 | National League · England |
| 2010-03-30 | AFC WimbledonvWrexhamNational League · England | 2–2 | National League · England |
| 2010-03-30 | OldhamvBrentfordLeague One · England | 2–3 | League One · England |
| 2010-03-30 | Inverness CvRoss CountyChampionship · Scotland | 3–0 | Championship · Scotland |
| 2010-03-29 | KaiserslauternvMunich 18602. Bundesliga · Germany | 4–0 | 2. Bundesliga · Germany |
| 2010-03-29 | Le HavrevGuingampLigue 2 · France | 2–1 | Ligue 2 · France |
| 2010-03-29 | Ath BilbaovSantanderLa Liga · Spain | 4–3 | La Liga · Spain |
| 2010-03-29 | Man CityvWiganPremier League · England | 3–0 | Premier League · England |
| 2010-03-29 | KayserisporvTrabzonsporSüper Lig · Turkey | 1–0 | Süper Lig · Turkey |
| 2010-03-29 | BarrowvHistonNational League · England | 0–0 | National League · England |
| 2010-03-29 | NewcastlevNott'm ForestChampionship · England | 2–0 | Championship · England |
| 2010-03-29 |  MonacovAuxerreLigue 1 · France | 0–0 | Ligue 1 · France |
| 2010-03-28 | HoffenheimvFreiburgBundesliga · Germany | 1–1 | Bundesliga · Germany |
| 2010-03-28 | AntalyasporvManisasporSüper Lig · Turkey | 0–0 | Süper Lig · Turkey |
| 2010-03-28 | OlympiakosvXanthiSuper League · Greece | 0–0 | Super League · Greece |
| 2010-03-28 | AachenvAugsburg2. Bundesliga · Germany | 4–0 | 2. Bundesliga · Germany |
| 2010-03-28 | La CorunavGetafeLa Liga · Spain | 1–3 | La Liga · Spain |
| 2010-03-28 | IraklisvGianninaSuper League · Greece | 2–0 | Super League · Greece |
| 2010-03-28 | Le MansvRennesLigue 1 · France | 1–3 | Ligue 1 · France |
| 2010-03-28 | St PaulivHansa Rostock2. Bundesliga · Germany | 2–0 | 2. Bundesliga · Germany |
| 2010-03-28 | HerculesvGimnasticLa Liga 2 · Spain | 0–1 | La Liga 2 · Spain |
| 2010-03-28 | NancyvLensLigue 1 · France | 5–1 | Ligue 1 · France |
| 2010-03-28 | ChievovParmaSerie A · Italy | 0–0 | Serie A · Italy |
| 2010-03-28 | Greuther FurthvAhlen2. Bundesliga · Germany | 3–1 | 2. Bundesliga · Germany |
| 2010-03-28 | AjaxvGroningenEredivisie · Netherlands | 3–0 | Eredivisie · Netherlands |
| 2010-03-28 | SalamancavNumanciaLa Liga 2 · Spain | 1–3 | La Liga 2 · Spain |
| 2010-03-28 | JuventusvAtalantaSerie A · Italy | 2–1 | Serie A · Italy |
| 2010-03-28 | VVV VenlovPSV EindhovenEredivisie · Netherlands | 2–4 | Eredivisie · Netherlands |
| 2010-03-28 | ArisvLevadeiakosSuper League · Greece | 1–0 | Super League · Greece |
| 2010-03-28 | Paris SGvBoulogneLigue 1 · France | 3–0 | Ligue 1 · France |
| 2010-03-28 | NapolivCataniaSerie A · Italy | 1–0 | Serie A · Italy |
| 2010-03-28 | BurnleyvBlackburnPremier League · England | 0–1 | Premier League · England |
| 2010-03-28 | OsasunavAlmeriaLa Liga · Spain | 1–0 | La Liga · Spain |
| 2010-03-28 | AEKvPanioniosSuper League · Greece | 1–1 | Super League · Greece |
| 2010-03-28 | LivornovBariSerie A · Italy | 1–1 | Serie A · Italy |
| 2010-03-28 | KavalavErgotelisSuper League · Greece | 1–3 | Super League · Greece |
| 2010-03-28 | Rio AvevOlhanensePrimeira Liga · Portugal | 1–5 | Primeira Liga · Portugal |
| 2010-03-28 | EspanolvSp GijonLa Liga · Spain | 0–0 | La Liga · Spain |
| 2010-03-28 | SienavGenoaSerie A · Italy | 0–0 | Serie A · Italy |
| 2010-03-28 | MilanvLazioSerie A · Italy | 1–1 | Serie A · Italy |
| 2010-03-28 | LillevMontpellierLigue 1 · France | 4–1 | Ligue 1 · France |
| 2010-03-28 | XerezvValladolidLa Liga · Spain | 3–0 | La Liga · Spain |
| 2010-03-28 | VillarrealvSevillaLa Liga · Spain | 3–0 | La Liga · Spain |
| 2010-03-28 | NijmegenvFeyenoordEredivisie · Netherlands | 0–0 | Eredivisie · Netherlands |
| 2010-03-28 | LiverpoolvSunderlandPremier League · England | 3–0 | Premier League · England |
| 2010-03-28 | AtromitosvPanathinaikosSuper League · Greece | 0–3 | Super League · Greece |
| 2010-03-28 | UtrechtvWaalwijkEredivisie · Netherlands | 2–0 | Eredivisie · Netherlands |