| 2021-08-28 | MillwallvBlackpoolChampionship · England | 2–1 | Championship · England |
| 2021-08-28 | Go Ahead EaglesvSparta RotterdamEredivisie · Netherlands | 2–0 | Eredivisie · Netherlands |
| 2021-08-28 | ShrewsburyvGillinghamLeague One · England | 2–1 | League One · England |
| 2021-08-28 | Dag and RedvBromleyNational League · England | 4–2 | National League · England |
| 2021-08-28 | VarbergvHalmstadAllsvenskan · Sweden | 1–0 | Allsvenskan · Sweden |
| 2021-08-28 | Gornik Z.vPiast GliwiceEkstraklasa · Poland | 0–1 | Ekstraklasa · Poland |
| 2021-08-28 | SalfordvNewport CountyLeague Two · England | 3–0 | League Two · England |
| 2021-08-28 | SociedadvLevanteLa Liga · Spain | 1–0 | La Liga · Spain |
| 2021-08-28 | BetisvReal MadridLa Liga · Spain | 0–1 | La Liga · Spain |
| 2021-08-28 | StenhousemuirvKelty HeartsLeague Two · Scotland | 1–4 | League Two · Scotland |
| 2021-08-28 | BarrowvBristol RvsLeague Two · England | 1–1 | League Two · England |
| 2021-08-28 | BrightonvEvertonPremier League · England | 0–2 | Premier League · England |
| 2021-08-28 | PlymouthvFleetwood TownLeague One · England | 1–1 | League One · England |
| 2021-08-28 | Yokohama F. MarinosvKashima AntlersJ1 League · Japan | 0–2 | J1 League · Japan |
| 2021-08-28 | St. GallenvZurichSuper League · Switzerland | 3–3 | Super League · Switzerland |
| 2021-08-28 | NicevBordeauxLigue 1 · France | 4–0 | Ligue 1 · France |
| 2021-08-28 | StirlingvAlbion RvsLeague Two · Scotland | 2–1 | League Two · Scotland |
| 2021-08-28 | ClydevCove RangersLeague One · Scotland | 2–1 | League One · Scotland |
| 2021-08-28 | NewcastlevSouthamptonPremier League · England | 2–2 | Premier League · England |
| 2021-08-28 | Boreham WoodvAldershotNational League · England | 1–0 | National League · England |
| 2021-08-28 | PartickvMortonChampionship · Scotland | 3–0 | Championship · Scotland |
| 2021-08-28 | CharleroivBeerschot VAPro League · Belgium | 5–2 | Pro League · Belgium |
| 2021-08-28 | Notts CountyvTorquayNational League · England | 1–1 | National League · England |
| 2021-08-28 | SouthendvStockportNational League · England | 0–1 | National League · England |
| 2021-08-28 | BielefeldvEin FrankfurtBundesliga · Germany | 1–1 | Bundesliga · Germany |
| 2021-08-28 | VizelavBoavistaPrimeira Liga · Portugal | 1–1 | Primeira Liga · Portugal |
| 2021-08-28 | Man CityvArsenalPremier League · England | 5–0 | Premier League · England |
| 2021-08-28 | SunderlandvWycombeLeague One · England | 3–1 | League One · England |
| 2021-08-28 | New York Red BullsvChicago FireMLS · United States | 0–1 | MLS · United States |
| 2021-08-28 | Gimnasia L.P.vHuracanLiga Profesional · Argentina | 0–0 | Liga Profesional · Argentina |
| 2021-08-28 | FamalicaovSp LisbonPrimeira Liga · Portugal | 1–1 | Primeira Liga · Portugal |
| 2021-08-28 | SwindonvMansfieldLeague Two · England | 1–0 | League Two · England |
| 2021-08-28 | AtalantavBolognaSerie A · Italy | 0–0 | Serie A · Italy |
| 2021-08-28 | ToulousevValenciennesLigue 2 · France | 1–0 | Ligue 2 · France |
| 2021-08-28 | AIKvOrebroAllsvenskan · Sweden | 2–0 | Allsvenskan · Sweden |
| 2021-08-28 | WiganvPortsmouthLeague One · England | 1–0 | League One · England |
| 2021-08-28 | SolihullvBarnetNational League · England | 1–1 | National League · England |
| 2021-08-28 | IpswichvAFC WimbledonLeague One · England | 2–2 | League One · England |
| 2021-08-28 | BanfieldvPlatenseLiga Profesional · Argentina | 2–4 | Liga Profesional · Argentina |
| 2021-08-28 | MainzvGreuther FurthBundesliga · Germany | 3–0 | Bundesliga · Germany |
| 2021-08-28 | HullvBournemouthChampionship · England | 0–0 | Championship · England |
| 2021-08-28 | SionvLuganoSuper League · Switzerland | 3–2 | Super League · Switzerland |
| 2021-08-28 | MjondalenvLillestromEliteserien · Norway | 1–2 | Eliteserien · Norway |
| 2021-08-28 | PSV EindhovenvGroningenEredivisie · Netherlands | 5–2 | Eredivisie · Netherlands |
| 2021-08-28 | DunfermlinevArbroathChampionship · Scotland | 0–3 | Championship · Scotland |
| 2021-08-28 | OviedovTenerifeLa Liga 2 · Spain | 0–0 | La Liga 2 · Spain |
| 2021-08-28 | Dundee UnitedvHeartsPremiership · Scotland | 0–2 | Premiership · Scotland |
| 2021-08-28 | ScunthorpevTranmereLeague Two · England | 1–0 | League Two · England |
| 2021-08-28 | HibernianvLivingstonPremiership · Scotland | 2–0 | Premiership · Scotland |
| 2021-08-28 | CardiffvBristol CityChampionship · England | 1–2 | Championship · England |