| 2010-04-10 | Le MansvMontpellierLigue 1 · France | 2–2 | Ligue 1 · France |
| 2010-04-10 | VillarrealvSp GijonLaLiga · Spain | 1–0 | LaLiga · Spain |
| 2010-04-10 | FalkirkvSt MirrenPremiership · Scotland | 2–1 | Premiership · Scotland |
| 2010-04-10 | ColchestervSwindonLeague One · England | 3–0 | League One · England |
| 2010-04-10 | CrewevShrewsburyLeague Two · England | 0–3 | League Two · England |
| 2010-04-10 | SouthamptonvCharltonLeague One · England | 1–0 | League One · England |
| 2010-04-10 | TriestinavBresciaSerie B · Italy | 0–1 | Serie B · Italy |
| 2010-04-10 | LeccevTorinoSerie B · Italy | 2–1 | Serie B · Italy |
| 2010-04-10 | RegginavEmpoliSerie B · Italy | 1–1 | Serie B · Italy |
| 2010-04-10 | HullvBurnleyPremier League · England | 1–4 | Premier League · England |
| 2010-04-10 | HamiltonvHibernianPremiership · Scotland | 4–1 | Premiership · Scotland |
| 2010-04-10 | HerthavStuttgartBundesliga · Germany | 0–1 | Bundesliga · Germany |
| 2010-04-10 | BesiktasvTrabzonsporSüper Lig · Türkiye | 0–0 | Süper Lig · Türkiye |
| 2010-04-10 | ChesterfieldvDag and RedLeague Two · England | 2–2 | League Two · England |
| 2010-04-10 | Crystal PalacevQPRChampionship · England | 0–2 | Championship · England |
| 2010-04-10 | StevenagevForest GreenNational League · England | 2–0 | National League · England |
| 2010-04-10 | TenerifevValladolidLaLiga · Spain | 0–0 | LaLiga · Spain |
| 2010-04-10 | SochauxvSt EtienneLigue 1 · France | 0–2 | Ligue 1 · France |
| 2010-04-10 | HerefordvGrimsbyLeague Two · England | 0–1 | League Two · England |
| 2010-04-10 | StenhousemuirvClydeLeague One · Scotland | 0–3 | League One · Scotland |
| 2010-04-10 | LivingstonvForfarLeague Two · Scotland | 2–3 | League Two · Scotland |
| 2010-04-10 | OldhamvBristol RvsLeague One · England | 2–1 | League One · England |
| 2010-04-10 | SalisburyvCambridgeNational League · England | 2–1 | National League · England |
| 2010-04-10 | AlbinoleffevCesenaSerie B · Italy | 1–2 | Serie B · Italy |
| 2010-04-10 | LincolnvBournemouthLeague Two · England | 2–1 | League Two · England |
| 2010-04-10 | DumbartonvEast FifeLeague One · Scotland | 0–1 | League One · Scotland |
| 2010-04-10 | AlbacetevGimnasticLaLiga 2 · Spain | 1–1 | LaLiga 2 · Spain |
| 2010-04-10 | NewcastlevBlackpoolChampionship · England | 4–1 | Championship · England |
| 2010-04-10 | GraysvWrexhamNational League · England | 0–2 | National League · England |
| 2010-04-10 | BarnetvMacclesfieldLeague Two · England | 1–2 | League Two · England |
| 2010-04-10 | ToulousevGrenobleLigue 1 · France | 4–0 | Ligue 1 · France |
| 2010-04-10 | MantovavPiacenzaSerie B · Italy | 1–1 | Serie B · Italy |
| 2010-04-10 | Rio AvevPortoPrimeira Liga · Portugal | 0–1 | Primeira Liga · Portugal |
| 2010-04-10 | StranraervQueens ParkLeague Two · Scotland | 0–0 | League Two · Scotland |
| 2010-04-10 | Real UnionvElcheLaLiga 2 · Spain | 0–3 | LaLiga 2 · Spain |
| 2010-04-10 | AlloavBrechinLeague One · Scotland | 2–3 | League One · Scotland |
| 2010-04-10 | LeiriavSp BragaPrimeira Liga · Portugal | 1–2 | Primeira Liga · Portugal |
| 2010-04-10 | PeterheadvCowdenbeathLeague One · Scotland | 1–0 | League One · Scotland |
| 2010-04-10 | AltrinchamvOxfordNational League · England | 0–1 | National League · England |
| 2010-04-10 | Bristol CityvSwanseaChampionship · England | 1–0 | Championship · England |
| 2010-04-10 | HoffenheimvFC KolnBundesliga · Germany | 0–2 | Bundesliga · Germany |
| 2010-04-10 | PrestonvScunthorpeChampionship · England | 3–2 | Championship · England |
| 2010-04-10 | LeedsvSouthendLeague One · England | 2–0 | League One · England |
| 2010-04-10 | PeterborovLeicesterChampionship · England | 1–2 | Championship · England |
| 2010-04-10 | XerezvGetafeLaLiga · Spain | 0–1 | LaLiga · Spain |
| 2010-04-10 | WatfordvPlymouthChampionship · England | 1–0 | Championship · England |
| 2010-04-10 | Port ValevAldershotLeague Two · England | 1–1 | League Two · England |
| 2010-04-10 | BarnsleyvDerbyChampionship · England | 0–0 | Championship · England |
| 2010-04-10 | HannovervSchalke 04Bundesliga · Germany | 4–2 | Bundesliga · Germany |
| 2010-04-10 | LeverkusenvBayern MunichBundesliga · Germany | 1–1 | Bundesliga · Germany |