| 2010-04-06 | LivingstonvMontroseLeague Two · Scotland | 1–0 | League Two · Scotland |
| 2010-04-06 | Ross CountyvQueen of SthChampionship · Scotland | 1–1 | Championship · Scotland |
| 2010-04-05 | Crystal PalacevPrestonChampionship · England | 3–1 | Championship · England |
| 2010-04-05 | Kettering TownvHistonNational League · England | 1–1 | National League · England |
| 2010-04-05 | Forest GreenvTamworthNational League · England | 3–4 | National League · England |
| 2010-04-05 | AldershotvRotherhamLeague Two · England | 3–0 | League Two · England |
| 2010-04-05 | SivassporvGalatasaraySüper Lig · Turkey | 1–1 | Süper Lig · Turkey |
| 2010-04-05 | BrentfordvExeterLeague One · England | 0–0 | League One · England |
| 2010-04-05 | WalsallvWycombeLeague One · England | 2–1 | League One · England |
| 2010-04-05 | AccringtonvGrimsbyLeague Two · England | 2–3 | League Two · England |
| 2010-04-05 | BuryvBurtonLeague Two · England | 3–0 | League Two · England |
| 2010-04-05 | CambridgevMansfieldNational League · England | 3–2 | National League · England |
| 2010-04-05 | ShrewsburyvNotts CountyLeague Two · England | 0–1 | League Two · England |
| 2010-04-05 | BarnsleyvPeterboroChampionship · England | 2–2 | Championship · England |
| 2010-04-05 | OxfordvSalisburyNational League · England | 1–0 | National League · England |
| 2010-04-05 | GillinghamvSouthendLeague One · England | 3–0 | League One · England |
| 2010-04-05 | WatfordvWest BromChampionship · England | 1–1 | Championship · England |
| 2010-04-05 | SwindonvTranmereLeague One · England | 3–0 | League One · England |
| 2010-04-05 | BlackpoolvDoncasterChampionship · England | 2–0 | Championship · England |
| 2010-04-05 | NavalvBenficaPrimeira Liga · Portugal | 2–4 | Primeira Liga · Portugal |
| 2010-04-05 | Bristol RvsvMilton Keynes DonsLeague One · England | 1–0 | League One · England |
| 2010-04-05 | DerbyvIpswichChampionship · England | 1–3 | Championship · England |
| 2010-04-05 | AFC WimbledonvStevenageNational League · England | 0–3 | National League · England |
| 2010-04-05 | CharltonvCarlisleLeague One · England | 1–0 | League One · England |
| 2010-04-05 | ColchestervMillwallLeague One · England | 1–2 | League One · England |
| 2010-04-05 | PlymouthvMiddlesbroughChampionship · England | 0–2 | Championship · England |
| 2010-04-05 | St JohnstonevDundee UnitedPremiership · Scotland | 0–1 | Premiership · Scotland |
| 2010-04-05 | Villarreal BvBetisLa Liga 2 · Spain | 2–2 | La Liga 2 · Spain |
| 2010-04-05 | Crawley TownvHayes & YeadingNational League · England | 1–0 | National League · England |
| 2010-04-05 | LeicestervQPRChampionship · England | 4–0 | Championship · England |
| 2010-04-05 | Nott'm ForestvCardiffChampionship · England | 0–0 | Championship · England |
| 2010-04-05 | LutonvGraysNational League · England | 6–0 | National League · England |
| 2010-04-05 | Port ValevDag and RedLeague Two · England | 3–1 | League Two · England |
| 2010-04-05 | HartlepoolvBrightonLeague One · England | 2–0 | League One · England |
| 2010-04-05 | EbbsfleetvEastbourne BoroughNational League · England | 3–2 | National League · England |
| 2010-04-05 | Fortuna DusseldorfvSt Pauli2. Bundesliga · Germany | 1–0 | 2. Bundesliga · Germany |
| 2010-04-05 | KidderminstervRushden & DNational League · England | 1–1 | National League · England |
| 2010-04-05 | YeovilvLeedsLeague One · England | 1–2 | League One · England |
| 2010-04-05 | ReadingvCoventryChampionship · England | 3–0 | Championship · England |
| 2010-04-05 | NorwichvStockportLeague One · England | 2–1 | League One · England |
| 2010-04-05 | BarnetvChesterfieldLeague Two · England | 3–1 | League Two · England |
| 2010-04-05 | BradfordvMacclesfieldLeague Two · England | 1–2 | League Two · England |
| 2010-04-05 | SouthamptonvLeyton OrientLeague One · England | 2–1 | League One · England |
| 2010-04-05 | YorkvAltrinchamNational League · England | 2–1 | National League · England |
| 2010-04-05 | StrasbourgvMetzLigue 2 · France | 1–1 | Ligue 2 · France |
| 2010-04-05 | SwanseavScunthorpeChampionship · England | 3–0 | Championship · England |
| 2010-04-05 | DarlingtonvHerefordLeague Two · England | 0–1 | League Two · England |
| 2010-04-05 | RochdalevBournemouthLeague Two · England | 0–0 | League Two · England |
| 2010-04-05 | CrewevCheltenhamLeague Two · England | 1–2 | League Two · England |
| 2010-04-05 | LincolnvMorecambeLeague Two · England | 1–3 | League Two · England |