| 2010-04-10 | HoffenheimvFC KolnBundesliga · Germany | 0–2 | Bundesliga · Germany |
| 2010-04-10 | PrestonvScunthorpeChampionship · England | 3–2 | Championship · England |
| 2010-04-10 | LeedsvSouthendLeague One · England | 2–0 | League One · England |
| 2010-04-10 | PeterborovLeicesterChampionship · England | 1–2 | Championship · England |
| 2010-04-10 | XerezvGetafeLa Liga · Spain | 0–1 | La Liga · Spain |
| 2010-04-10 | WatfordvPlymouthChampionship · England | 1–0 | Championship · England |
| 2010-04-10 | Port ValevAldershotLeague Two · England | 1–1 | League Two · England |
| 2010-04-10 | BarnsleyvDerbyChampionship · England | 0–0 | Championship · England |
| 2010-04-10 | HannovervSchalke 04Bundesliga · Germany | 4–2 | Bundesliga · Germany |
| 2010-04-10 | LeverkusenvBayern MunichBundesliga · Germany | 1–1 | Bundesliga · Germany |
| 2010-04-10 | WycombevHartlepoolLeague One · England | 2–0 | League One · England |
| 2010-04-10 | DoncastervWest BromChampionship · England | 2–3 | Championship · England |
| 2010-04-10 | CottbusvFortuna Dusseldorf2. Bundesliga · Germany | 4–2 | 2. Bundesliga · Germany |
| 2010-04-10 | CheltenhamvBuryLeague Two · England | 5–2 | League Two · England |
| 2010-04-10 | TorquayvRochdaleLeague Two · England | 5–0 | League Two · England |
| 2010-04-10 | Eastbourne BoroughvYorkNational League · England | 3–1 | National League · England |
| 2010-04-10 | Albion RvsvElginLeague Two · Scotland | 1–2 | League Two · Scotland |
| 2010-04-10 | Sheffield UnitedvCoventryChampionship · England | 1–0 | Championship · England |
| 2010-04-10 | Hayes & YeadingvBarrowNational League · England | 1–1 | National League · England |
| 2010-04-10 |  MonacovValenciennesLigue 1 · France | 2–1 | Ligue 1 · France |
| 2010-04-10 | Villarreal BvGironaLa Liga 2 · Spain | 2–0 | La Liga 2 · Spain |
| 2010-04-10 | CardiffvReadingChampionship · England | 0–0 | Championship · England |
| 2010-04-10 | Nott'm ForestvIpswichChampionship · England | 3–0 | Championship · England |
| 2010-04-10 | VallecanovLas PalmasLa Liga 2 · Spain | 3–2 | La Liga 2 · Spain |
| 2010-04-10 | LensvBoulogneLigue 1 · France | 3–0 | Ligue 1 · France |
| 2010-04-10 | NorwichvMilton Keynes DonsLeague One · England | 1–1 | League One · England |
| 2010-04-10 | BrentfordvHuddersfieldLeague One · England | 3–0 | League One · England |
| 2010-04-10 | Werder BremenvFreiburgBundesliga · Germany | 4–0 | Bundesliga · Germany |
| 2010-04-10 | BetisvCadizLa Liga 2 · Spain | 4–0 | La Liga 2 · Spain |
| 2010-04-10 | HistonvCrawley TownNational League · England | 0–1 | National League · England |
| 2010-04-10 | BrightonvCarlisleLeague One · England | 1–2 | League One · England |
| 2010-04-10 | ArbroathvStirlingLeague One · Scotland | 2–4 | League One · Scotland |
| 2010-04-10 | BerwickvEast StirlingLeague Two · Scotland | 2–2 | League Two · Scotland |
| 2010-04-10 | NorthamptonvNotts CountyLeague Two · England | 0–1 | League Two · England |
| 2010-04-10 | MainzvDortmundBundesliga · Germany | 1–0 | Bundesliga · Germany |
| 2010-04-10 | AnconavCrotoneSerie B · Italy | 0–1 | Serie B · Italy |
| 2010-04-10 | MiddlesbroughvSheffield WedsChampionship · England | 1–0 | Championship · England |
| 2010-04-10 | MansfieldvAFC WimbledonNational League · England | 0–1 | National League · England |
| 2010-04-10 | MorecambevRotherhamLeague Two · England | 2–0 | League Two · England |
| 2010-04-10 | ModenavSassuoloSerie B · Italy | 1–1 | Serie B · Italy |
| 2010-04-10 | NAC BredavRoda JCEredivisie · Netherlands | 1–0 | Eredivisie · Netherlands |
| 2010-04-10 | KaiserslauternvUnion Berlin2. Bundesliga · Germany | 1–1 | 2. Bundesliga · Germany |
| 2010-04-10 | WalsallvTranmereLeague One · England | 2–1 | League One · England |
| 2010-04-10 | MalagavSevillaLa Liga · Spain | 1–2 | La Liga · Spain |
| 2010-04-10 | HeartsvKilmarnockPremiership · Scotland | 1–0 | Premiership · Scotland |
| 2010-04-10 | NijmegenvAZ AlkmaarEredivisie · Netherlands | 0–0 | Eredivisie · Netherlands |
| 2010-04-10 | GatesheadvKidderminsterNational League · England | 0–2 | National League · England |
| 2010-04-10 | FiorentinavInterSerie A · Italy | 2–2 | Serie A · Italy |
| 2010-04-10 | BurtonvBradfordLeague Two · England | 1–1 | League Two · England |
| 2010-04-10 | MillwallvGillinghamLeague One · England | 4–0 | League One · England |