| 2009-04-18 | Aston VillavWest HamPremier League · England | 1–1 | Premier League · England |
| 2009-04-18 | BerwickvStenhousemuirLeague Two · Scotland | 0–3 | League Two · Scotland |
| 2009-04-18 | Leyton OrientvNorthamptonLeague One · England | 1–3 | League One · England |
| 2009-04-18 | GuimaraesvSp LisbonPrimeira Liga · Portugal | 1–2 | Primeira Liga · Portugal |
| 2009-04-18 | Dundee UnitedvKilmarnockPremiership · Scotland | 0–0 | Premiership · Scotland |
| 2009-04-18 | PlymouthvDoncasterChampionship · England | 0–3 | Championship · England |
| 2009-04-18 | East StirlingvMontroseLeague Two · Scotland | 2–1 | League Two · Scotland |
| 2009-04-18 | VallecanovCeltaLa Liga 2 · Spain | 4–1 | La Liga 2 · Spain |
| 2009-04-18 | Nott'm ForestvCoventryChampionship · England | 1–0 | Championship · England |
| 2009-04-18 | LeedsvTranmereLeague One · England | 3–1 | League One · England |
| 2009-04-18 | ElchevSevilla BLa Liga 2 · Spain | 2–0 | La Liga 2 · Spain |
| 2009-04-18 | MiddlesbroughvFulhamPremier League · England | 0–0 | Premier League · England |
| 2009-04-18 | PrestonvCardiffChampionship · England | 6–0 | Championship · England |
| 2009-04-18 | BarrowvCrawley TownNational League · England | 3–3 | National League · England |
| 2009-04-18 | LincolnvExeterLeague Two · England | 0–1 | League Two · England |
| 2009-04-18 | StirlingvRaith RvsLeague One · Scotland | 0–1 | League One · Scotland |
| 2009-04-18 | MorecambevNotts CountyLeague Two · England | 1–0 | League Two · England |
| 2009-04-18 | CowdenbeathvDumbartonLeague Two · Scotland | 0–0 | League Two · Scotland |
| 2009-04-18 | WaregemvKortrijkPro League · Belgium | 5–1 | Pro League · Belgium |
| 2009-04-18 | StranraervPeterheadLeague One · Scotland | 0–1 | League One · Scotland |
| 2009-04-18 | BarnetvLutonLeague Two · England | 1–1 | League Two · England |
| 2009-04-18 | ChestervBournemouthLeague Two · England | 0–2 | League Two · England |
| 2009-04-18 | NijmegenvRoda JCEredivisie · Netherlands | 1–1 | Eredivisie · Netherlands |
| 2009-04-18 | LevantevHuescaLa Liga 2 · Spain | 1–0 | La Liga 2 · Spain |
| 2009-04-18 | GetafevBarcelonaLa Liga · Spain | 0–1 | La Liga · Spain |
| 2009-04-18 | NorthwichvLewesNational League · England | 3–0 | National League · England |
| 2009-04-18 | SalernitanavAlbinoleffeSerie B · Italy | 4–2 | Serie B · Italy |
| 2009-04-18 | FeyenoordvTwenteEredivisie · Netherlands | 1–0 | Eredivisie · Netherlands |
| 2009-04-18 | YeovilvHartlepoolLeague One · England | 2–3 | League One · England |
| 2009-04-18 | Ath BilbaovLa CorunaLa Liga · Spain | 0–1 | La Liga · Spain |
| 2009-04-18 | CharltonvBlackpoolChampionship · England | 2–2 | Championship · England |
| 2009-04-18 | CrewevCheltenhamLeague One · England | 1–2 | League One · England |
| 2009-04-18 |  MonacovRennesLigue 1 · France | 3–1 | Ligue 1 · France |
| 2009-04-18 | PortsmouthvBoltonPremier League · England | 1–0 | Premier League · England |
| 2009-04-18 | MurciavCastellonLa Liga 2 · Spain | 2–2 | La Liga 2 · Spain |
| 2009-04-18 | AnkarasporvFenerbahceSüper Lig · Turkey | 1–0 | Süper Lig · Turkey |
| 2009-04-18 | Kettering TownvWrexhamNational League · England | 1–0 | National League · England |
| 2009-04-18 | LokerenvGerminalPro League · Belgium | 1–0 | Pro League · Belgium |
| 2009-04-18 | BrightonvOldhamLeague One · England | 3–1 | League One · England |
| 2009-04-18 | BuryvMacclesfieldLeague Two · England | 3–0 | League Two · England |
| 2009-04-18 | CarlislevSwindonLeague One · England | 1–1 | League One · England |
| 2009-04-18 | BochumvDortmundBundesliga · Germany | 0–2 | Bundesliga · Germany |
| 2009-04-18 | BielefeldvBayern MunichBundesliga · Germany | 0–1 | Bundesliga · Germany |
| 2009-04-18 | LivornovParmaSerie B · Italy | 2–2 | Serie B · Italy |
| 2009-04-18 | ReadingvBarnsleyChampionship · England | 0–0 | Championship · England |
| 2009-04-18 | TubizevCharleroiPro League · Belgium | 3–1 | Pro League · Belgium |
| 2009-04-18 | WolvesvQPRChampionship · England | 1–0 | Championship · England |
| 2009-04-18 | St EtiennevLilleLigue 1 · France | 2–1 | Ligue 1 · France |
| 2009-04-18 | SouthendvLeicesterLeague One · England | 0–2 | League One · England |
| 2009-04-18 | GenoavLazioSerie A · Italy | 0–1 | Serie A · Italy |