| 2010-04-13 | BerwickvLivingstonLeague Two · Scotland | 1–1 | League Two · Scotland |
| 2010-04-13 | CarlislevLeedsLeague One · England | 1–3 | League One · England |
| 2010-04-13 | RochdalevDarlingtonLeague Two · England | 0–1 | League Two · England |
| 2010-04-13 | ValladolidvSevillaLa Liga · Spain | 2–1 | La Liga · Spain |
| 2010-04-13 | VicenzavAnconaSerie B · Italy | 2–2 | Serie B · Italy |
| 2010-04-13 | AldershotvTorquayLeague Two · England | 0–2 | League Two · England |
| 2010-04-13 | Annan AthleticvMontroseLeague Two · Scotland | 0–0 | League Two · Scotland |
| 2010-04-13 | CharltonvColchesterLeague One · England | 1–0 | League One · England |
| 2010-04-13 | GillinghamvBrightonLeague One · England | 1–1 | League One · England |
| 2010-04-13 | BuryvCreweLeague Two · England | 3–0 | League Two · England |
| 2010-04-13 | SalisburyvHayes & YeadingNational League · England | 3–1 | National League · England |
| 2010-04-13 | HuddersfieldvWalsallLeague One · England | 4–3 | League One · England |
| 2010-04-13 | BournemouthvHerefordLeague Two · England | 2–1 | League Two · England |
| 2010-04-13 | RotherhamvNorthamptonLeague Two · England | 1–0 | League Two · England |
| 2010-04-13 | HartlepoolvStockportLeague One · England | 3–0 | League One · England |
| 2010-04-13 | SouthendvBrentfordLeague One · England | 2–2 | League One · England |
| 2010-04-13 | Bristol RvsvSouthamptonLeague One · England | 1–5 | League One · England |
| 2010-04-13 | AscolivTriestinaSerie B · Italy | 1–0 | Serie B · Italy |
| 2010-04-13 | AccringtonvPort ValeLeague Two · England | 1–2 | League Two · England |
| 2010-04-13 | MacclesfieldvBurtonLeague Two · England | 1–1 | League Two · England |
| 2010-04-13 | Sp GijonvTenerifeLa Liga · Spain | 0–2 | La Liga · Spain |
| 2010-04-13 | TranmerevWycombeLeague One · England | 0–3 | League One · England |
| 2010-04-13 | Dag and RedvBarnetLeague Two · England | 4–1 | League Two · England |
| 2010-04-13 | Milton Keynes DonsvOldhamLeague One · England | 0–0 | League One · England |
| 2010-04-13 | AFC WimbledonvGraysNational League · England | 0–2 | National League · England |
| 2010-04-13 | ChelseavBoltonPremier League · England | 1–0 | Premier League · England |
| 2010-04-13 | Forest GreenvCrawley TownNational League · England | 1–0 | National League · England |
| 2010-04-13 | AlbinoleffevGrossetoSerie B · Italy | 1–1 | Serie B · Italy |
| 2010-04-13 | DumbartonvClydeLeague One · Scotland | 3–3 | League One · Scotland |
| 2010-04-13 | GallipolivCittadellaSerie B · Italy | 1–2 | Serie B · Italy |
| 2010-04-13 | Notts CountyvLincolnLeague Two · England | 3–1 | League Two · England |
| 2010-04-13 | PadovavLecceSerie B · Italy | 1–1 | Serie B · Italy |
| 2010-04-13 | CelticvMotherwellPremiership · Scotland | 2–1 | Premiership · Scotland |
| 2010-04-13 | EmpolivSalernitanaSerie B · Italy | 5–2 | Serie B · Italy |
| 2010-04-13 | AZ AlkmaarvTwenteEredivisie · Netherlands | 1–0 | Eredivisie · Netherlands |
| 2010-04-13 | BradfordvMorecambeLeague Two · England | 2–0 | League Two · England |
| 2010-04-13 | OxfordvCambridgeNational League · England | 0–0 | National League · England |
| 2010-04-13 | VitessevNAC BredaEredivisie · Netherlands | 1–1 | Eredivisie · Netherlands |
| 2010-04-13 | SassuolovRegginaSerie B · Italy | 0–1 | Serie B · Italy |
| 2010-04-13 | CesenavMantovaSerie B · Italy | 3–1 | Serie B · Italy |
| 2010-04-13 | GetafevVillarrealLa Liga · Spain | 3–0 | La Liga · Spain |
| 2010-04-13 | BresciavFrosinoneSerie B · Italy | 3–1 | Serie B · Italy |
| 2010-04-13 | LutonvHistonNational League · England | 6–3 | National League · England |
| 2010-04-13 | MansfieldvRushden & DNational League · England | 3–2 | National League · England |
| 2010-04-13 | GrimsbyvChesterfieldLeague Two · England | 2–2 | League Two · England |
| 2010-04-13 | TorinovPiacenzaSerie B · Italy | 1–1 | Serie B · Italy |
| 2010-04-13 | HeraclesvWaalwijkEredivisie · Netherlands | 4–1 | Eredivisie · Netherlands |
| 2010-04-13 | YorkvBarrowNational League · England | 3–0 | National League · England |
| 2010-04-13 | ShrewsburyvCheltenhamLeague Two · England | 0–0 | League Two · England |
| 2010-04-13 | YeovilvMillwallLeague One · England | 1–1 | League One · England |